PMID- 33327643
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201229
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 9
IP  - 12
DP  - 2020 Dec 14
TI  - Melatonin Ameliorates Inflammation and Oxidative Stress by Suppressing the 
      p38MAPK Signaling Pathway in LPS-Induced Sheep Orchitis.
LID - 10.3390/antiox9121277 [doi]
LID - 1277
AB  - Gram-negative bacterial infections of the testis can lead to infectious orchitis, 
      which negatively influences steroid hormone synthesis and spermatogenesis. 
      Lipopolysaccharide (LPS), a major component of the Gram-negative bacterial cell 
      wall, acts via toll like receptors 4 (TLR4) to trigger innate immune responses 
      and activate nuclear factor kappa B signaling. The protective mechanisms of 
      melatonin on LPS-induced infectious orchitis have not been reported. Herein, we 
      developed an LPS-induced sheep infectious orchitis model. In this model, the 
      phagocytic activity of testicular macrophages (TM) was enhanced after melatonin 
      treatment. Moreover, we found that melatonin suppressed secretion of TM 
      pro-inflammatory factors by suppressing the p38MAPK pathway and promoting Leydig 
      cell testosterone secretion. Expressions of GTP cyclohydrolase-I and NADPH 
      oxidase-2 were reduced by melatonin while heme oxygenase-1 expression was 
      up-regulated. Thus, melatonin reduced the severity of LPS-induced orchitis by 
      stimulating antioxidant activity. The results of this study provide a reference 
      for the treatment of acute infectious orchitis.
FAU - Deng, Shou-Long
AU  - Deng SL
AD  - CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of 
      Genomics, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Zhang, Bao-Lu
AU  - Zhang BL
AD  - Marine Consulting Center of Natural Resources of the People's Republic of China, 
      Beijing 100071, China.
FAU - Reiter, Russel J
AU  - Reiter RJ
AD  - Department of Cell Systems and Anatomy, UT Health San Antonio, San Antonio, TX 
      78229, USA.
FAU - Liu, Yi-Xun
AU  - Liu YX
AD  - State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, 
      Chinese Academy of Sciences, Beijing 100101, China.
LA  - eng
PT  - Journal Article
DEP - 20201214
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC7765110
OTO - NOTNLM
OT  - TLR4
OT  - melatonin
OT  - p38MAPK
OT  - sheep
OT  - testicular macrophages
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/18 06:00
MHDA- 2020/12/18 06:01
PMCR- 2020/12/14
CRDT- 2020/12/17 01:03
PHST- 2020/11/23 00:00 [received]
PHST- 2020/12/10 00:00 [revised]
PHST- 2020/12/11 00:00 [accepted]
PHST- 2020/12/17 01:03 [entrez]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2020/12/18 06:01 [medline]
PHST- 2020/12/14 00:00 [pmc-release]
AID - antiox9121277 [pii]
AID - antioxidants-09-01277 [pii]
AID - 10.3390/antiox9121277 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2020 Dec 14;9(12):1277. doi: 10.3390/antiox9121277.