PMID- 33328612
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210331
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 16
IP  - 2
DP  - 2021 Feb
TI  - A surgical mouse model of neonatal pressure overload by transverse aortic 
      constriction.
PG  - 775-790
LID - 10.1038/s41596-020-00434-9 [doi]
AB  - Cardiac disease is the main cause of death worldwide. Insufficient regeneration 
      of the adult mammalian heart is a major driver of cardiac morbidity and 
      mortality. Cardiac regeneration occurs in early postnatal mice, thus 
      understanding mechanisms of mammalian cardiac regeneration could facilitate the 
      development of novel therapeutic strategies. Here, we provide a detailed 
      description of a neonatal mouse model of pressure overload by transverse aortic 
      constriction (nTAC) that can be applied at postnatal days 1 and 7. We have 
      previously used this model to demonstrate that mice are able to fully adapt to 
      pressure overload following nTAC on postnatal day 1. In contrast, when nTAC is 
      applied in the non-regenerative phase (at postnatal day 7), it is associated with 
      a maladaptive response similar to that seen when transverse aortic constriction 
      (TAC) is applied to adult mice. Once a user is experienced in nTAC surgery, the 
      procedure can be completed in less than 10 min per mouse. We anticipate that this 
      model will facilitate the discovery of therapeutic targets to treat patients or 
      prevent pressure overload-induced cardiac failure in the future.
FAU - Malek Mohammadi, Mona
AU  - Malek Mohammadi M
AUID- ORCID: 0000-0001-8410-7865
AD  - Department of Cardiovascular Physiology, European Center for Angioscience, 
      Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. 
      mona.malekmohammadi@uni-bonn.de.
AD  - German Center for Cardiovascular Research (DZHK), partner site Heidelberg, 
      Mannheim, Germany. mona.malekmohammadi@uni-bonn.de.
FAU - Abouissa, Aya
AU  - Abouissa A
AD  - Department of Cardiovascular Physiology, European Center for Angioscience, 
      Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.
AD  - German Center for Cardiovascular Research (DZHK), partner site Heidelberg, 
      Mannheim, Germany.
FAU - Heineke, Joerg
AU  - Heineke J
AUID- ORCID: 0000-0002-1541-3030
AD  - Department of Cardiovascular Physiology, European Center for Angioscience, 
      Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. 
      Joerg.Heineke@medma.uni-heidelberg.de.
AD  - German Center for Cardiovascular Research (DZHK), partner site Heidelberg, 
      Mannheim, Germany. Joerg.Heineke@medma.uni-heidelberg.de.
LA  - eng
GR  - EXC62/3/Deutsche Forschungsgemeinschaft (German Research Foundation)/
GR  - HE3658/6-1/Deutsche Forschungsgemeinschaft (German Research Foundation)/
GR  - HE 3658/11-1/Deutsche Forschungsgemeinschaft (German Research Foundation)/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201216
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
SB  - IM
MH  - Animals
MH  - Animals, Newborn/physiology/surgery
MH  - Aorta/surgery
MH  - Blood Pressure/physiology
MH  - Constriction
MH  - Disease Models, Animal
MH  - Female
MH  - Heart/*physiology
MH  - Heart Diseases/metabolism/physiopathology/*surgery
MH  - Heart Failure/etiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Myocytes, Cardiac/physiology
MH  - Regeneration/*physiology
EDAT- 2020/12/18 06:00
MHDA- 2021/03/09 06:00
CRDT- 2020/12/17 05:44
PHST- 2020/01/30 00:00 [received]
PHST- 2020/10/06 00:00 [accepted]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/12/17 05:44 [entrez]
AID - 10.1038/s41596-020-00434-9 [pii]
AID - 10.1038/s41596-020-00434-9 [doi]
PST - ppublish
SO  - Nat Protoc. 2021 Feb;16(2):775-790. doi: 10.1038/s41596-020-00434-9. Epub 2020 
      Dec 16.