PMID- 33329494
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201218
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Anti-infective Effects of a Fish-Derived Antimicrobial Peptide Against 
      Drug-Resistant Bacteria and Its Synergistic Effects With Antibiotic.
PG  - 602412
LID - 10.3389/fmicb.2020.602412 [doi]
LID - 602412
AB  - Antimicrobial peptides (AMPs) play pivotal roles in protecting against microbial 
      infection in fish. However, AMPs from topmouth culter (Erythroculter 
      ilishaeformis) are rarely known. In our study, we isolated an AMP from the head 
      kidney of topmouth culter, which belonged to liver-expressed antimicrobial 
      peptide 2 (LEAP-2) family. Topmouth culter LEAP-2 showed inhibitory effects on 
      aquatic bacterial growth, including antibiotic-resistant bacteria, with minimal 
      inhibitory concentration values ranging from 18.75 to 150 mug/ml. It was lethal 
      for Aeromonas hydrophila (resistant to ampicillin), and took less than 60 min to 
      kill A. hydrophila at a concentration of 5 x MIC. Scanning electron microscope 
      (SEM) and SYTOX Green uptake assay indicated that it impaired the integrity of 
      bacterial membrane by eliciting pore formation, thereby increasing the 
      permeabilization of bacterial membrane. In addition, it showed none inducible 
      drug resistance to aquatic bacteria. Interestingly, it efficiently delayed 
      ampicillin-induced drug resistance in Vibrio parahaemolyticus (sensitive to 
      ampicillin) and sensitized ampicillin-resistant bacteria to ampicillin. The 
      chequerboard assay indicated that topmouth culter LEAP-2 generated synergistic 
      effects with ampicillin, indicating the combinational usage potential of topmouth 
      culter LEAP-2 with antibiotics. As expected, topmouth culter LEAP-2 significantly 
      alleviated ampicillin-resistant A. hydrophila infection in vivo, and enhanced the 
      therapeutic efficacy of ampicillin against A. hydrophila in vivo. Our findings 
      provide a fish innate immune system-derived peptide candidate for the substitute 
      of antibiotics and highlight its potential for application in 
      antibiotic-resistant bacterial infection in aquaculture industry.
CI  - Copyright (c) 2020 Chen, Wu, Cheng, Dai, Wang, Yang, Xiong, Xu and Wei.
FAU - Chen, Yue
AU  - Chen Y
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, China.
FAU - Wu, Jing
AU  - Wu J
AD  - School of Basic Medical Sciences, Kunming Medical University, Kunming, China.
FAU - Cheng, Honglan
AU  - Cheng H
AD  - The Second Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Dai, Yue
AU  - Dai Y
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, China.
FAU - Wang, Yipeng
AU  - Wang Y
AD  - Department of Biopharmaceuticals, College of Pharmaceutical Sciences, Soochow 
      University, Suzhou, China.
FAU - Yang, Hailong
AU  - Yang H
AD  - The Second Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Xiong, Fei
AU  - Xiong F
AD  - The Second Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Xu, Wei
AU  - Xu W
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, China.
FAU - Wei, Lin
AU  - Wei L
AD  - Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and 
      Medical Sciences, Soochow University, Suzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20201123
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7719739
OTO - NOTNLM
OT  - Erythroculter ilishaeformis
OT  - antimicrobial peptide
OT  - aquatic bacteria
OT  - drug resistance
OT  - liver-expressed antimicrobial peptide 2
OT  - synergistic effect
EDAT- 2020/12/18 06:00
MHDA- 2020/12/18 06:01
PMCR- 2020/11/23
CRDT- 2020/12/17 05:50
PHST- 2020/09/04 00:00 [received]
PHST- 2020/10/28 00:00 [accepted]
PHST- 2020/12/17 05:50 [entrez]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2020/12/18 06:01 [medline]
PHST- 2020/11/23 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.602412 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Nov 23;11:602412. doi: 10.3389/fmicb.2020.602412. 
      eCollection 2020.