PMID- 33331361
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1759-9679 (Electronic)
IS  - 1759-9660 (Linking)
VI  - 13
IP  - 2
DP  - 2021 Jan 21
TI  - A reliable and accurate UHPLC-MS/MS method for screening of Aspergillus, 
      Penicillium and Alternaria mycotoxins in orange, grape and apple juices.
PG  - 192-201
LID - 10.1039/d0ay01787f [doi]
AB  - An ultra-high performance liquid chromatography-tandem mass spectrometry 
      (UHPLC-MS/MS) method was developed for simultaneous determination of 15 
      mycotoxins, including aflatoxins (B1, B2, G1, and G2), ochratoxins (A, B, and C), 
      citrinin, patulin, and emerging Alternaria toxins (alternariol, alternariol 
      monomethyl ether, altenuene, tentoxin, tenuazonic acid, and altenusin) in orange, 
      grape and apple juices. Different extraction approaches, sorbents, 
      chromatographic columns and mobile phases were investigated for establishment of 
      an optimal QuEChERS procedure and UHPLC-MS/MS conditions. Recoveries were in the 
      range of 74-110%, and the limits of detection (LODs) and limits of quantification 
      (LOQs) ranged from 0.05 to 0.1 ng mL-1 and from 0.1 to 5.0 ng mL-1, respectively. 
      Matrix effects were evaluated and matrix-matched calibration curves were used to 
      compensate for matrix effects and achieve accurate quantification. The 
      correlation coefficients (R2) of linearity were higher than 0.99 and the relative 
      standard deviations (RSDs) of intra- and inter-day precision were under 13%. The 
      method was subsequently applied to 22 fruit juice samples. The high frequencies 
      (90.9%) of mycotoxins not only proved the reliability and sensitivity of the 
      currently established method, but also demonstrated that fruit juices are 
      susceptible to different mycotoxins, which need to be continuously monitored in 
      the future.
FAU - Guo, Wenbo
AU  - Guo W
AD  - Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory 
      of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 
      Shanghai 201403, China. hanzheng_ok@163.com.
FAU - Yang, Junhua
AU  - Yang J
AD  - Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory 
      of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 
      Shanghai 201403, China. hanzheng_ok@163.com.
FAU - Niu, Xueke
AU  - Niu X
AD  - Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory 
      of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 
      Shanghai 201403, China. hanzheng_ok@163.com.
FAU - Tangni, Emmanuel K
AU  - Tangni EK
AD  - Organic Contaminants and Additives, Sciensano, Leuvensesteenweg 17, Tervuren 
      3080, Belgium. emmanuel.tangni@sciensano.be.
FAU - Zhao, Zhihui
AU  - Zhao Z
AD  - Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory 
      of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 
      Shanghai 201403, China. hanzheng_ok@163.com.
FAU - Han, Zheng
AU  - Han Z
AUID- ORCID: 0000-0001-9702-2297
AD  - Institute for Agro-food Standards and Testing Technology, Shanghai Key Laboratory 
      of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, 
      Shanghai 201403, China. hanzheng_ok@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Anal Methods
JT  - Analytical methods : advancing methods and applications
JID - 101519733
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Alternaria
MH  - Aspergillus
MH  - Chromatography, High Pressure Liquid
MH  - *Citrus sinensis
MH  - Fruit and Vegetable Juices
MH  - *Malus
MH  - *Mycotoxins/analysis
MH  - *Penicillium
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry
MH  - *Vitis
EDAT- 2020/12/18 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/12/17 08:42
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/12/17 08:42 [entrez]
AID - 10.1039/d0ay01787f [doi]
PST - ppublish
SO  - Anal Methods. 2021 Jan 21;13(2):192-201. doi: 10.1039/d0ay01787f.