PMID- 33332775
OWN - NLM
STAT- MEDLINE
DCOM- 20210915
LR  - 20210915
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 25
IP  - 1
DP  - 2021 Jan
TI  - Sustained expression of MCP-1 induced low wall shear stress loading in 
      conjunction with turbulent flow on endothelial cells of intracranial aneurysm.
PG  - 110-119
LID - 10.1111/jcmm.15868 [doi]
AB  - Shear stress was reported to regulate the expression of AC007362, but its 
      underlying mechanisms remain to be explored. In this study, to isolate 
      endothelial cells of blood vessels, unruptured and ruptured intracranial aneurysm 
      (IA) tissues were collected from IA patients. Subsequently, quantitative 
      real-time PCR (qRT-PCR), Western blot and luciferase assay were performed to 
      investigate the relationships between AC007362, miRNAs-493 and monocyte 
      chemoattractant protein-1 (MCP-1) in human umbilical vein endothelial cells 
      (HUVECs) exposed to shear stress. Reduced representation bisulphite sequencing 
      (RRBS) was performed to assess the level of DNA methylation in AC007362 promoter. 
      Accordingly, AC007362 and MCP-1 were significantly up-regulated while miR-493 was 
      significantly down-regulated in HUVECs exposed to shear stress. AC007362 could 
      suppress the miR-493 expression and elevate the MCP-1 expression, and miR-493 was 
      shown to respectively target AC007362 and MCP-1. Moreover, shear stress in HUVECs 
      led to the down-regulated DNA methyltransferase 1 (DNMT1), as well as the 
      decreased DNA methylation level of AC007362 promoter. Similar results were also 
      observed in ruptured IA tissues when compared with unruptured IA tissues. In 
      conclusion, this study presented a deep insight into the operation of the 
      regulatory network of AC007362, miR-493 and MCP-1 upon shear stress. Under shear 
      stress, the expression of AC007362 was enhanced by the inhibited promoter DNA 
      methylation, while the expression of MCP-1 was enhanced by sponging the 
      expression of miR-493.
CI  - (c) 2020 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Chu, Cheng
AU  - Chu C
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Xu, Gang
AU  - Xu G
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Li, Xiaocong
AU  - Li X
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Duan, Zuowei
AU  - Duan Z
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Tao, Lihong
AU  - Tao L
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Cai, Hongxia
AU  - Cai H
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Yang, Ming
AU  - Yang M
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Zhang, Xinjiang
AU  - Zhang X
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Zheng, Yanyu
AU  - Zheng Y
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Shi, Hongcan
AU  - Shi H
AUID- ORCID: 0000-0003-2228-4828
AD  - Department of Neurology, Affiliated Hospital of Yangzhou University, Yangzhou 
      University, Yangzhou, China.
FAU - Li, Xiaoyu
AU  - Li X
AD  - Department of Pathophysiology, Key Laboratory of Cardiovascular Disease and 
      Molecular Intervention, Nanjing Medical University, Nanjing, China.
LA  - eng
GR  - YZ2017111/Yangzhou Natural Science Foundation-Youth Fund Project/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201217
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Chemokine CCL2)
RN  - 0 (MIRN493 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Base Sequence
MH  - Chemokine CCL2/genetics/*metabolism
MH  - DNA Methylation/genetics
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells/metabolism/*pathology
MH  - Humans
MH  - Intracranial Aneurysm/genetics/*pathology
MH  - Male
MH  - MicroRNAs/genetics/metabolism
MH  - Middle Aged
MH  - Promoter Regions, Genetic/genetics
MH  - *Rheology
MH  - *Stress, Mechanical
MH  - Up-Regulation/genetics
PMC - PMC7810920
OTO - NOTNLM
OT  - IA
OT  - MCP-1
OT  - miRNA
OT  - shear stress
COIS- None.
EDAT- 2020/12/18 06:00
MHDA- 2021/09/16 06:00
PMCR- 2021/01/01
CRDT- 2020/12/17 17:22
PHST- 2020/04/19 00:00 [received]
PHST- 2020/07/30 00:00 [revised]
PHST- 2020/08/24 00:00 [accepted]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2021/09/16 06:00 [medline]
PHST- 2020/12/17 17:22 [entrez]
PHST- 2021/01/01 00:00 [pmc-release]
AID - JCMM15868 [pii]
AID - 10.1111/jcmm.15868 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2021 Jan;25(1):110-119. doi: 10.1111/jcmm.15868. Epub 2020 Dec 
      17.