PMID- 33333215
OWN - NLM
STAT- MEDLINE
DCOM- 20210121
LR  - 20210121
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 771
DP  - 2021 Mar 1
TI  - Puerarin alleviates coronary heart disease via suppressing inflammation in a rat 
      model.
PG  - 145354
LID - S0378-1119(20)31023-4 [pii]
LID - 10.1016/j.gene.2020.145354 [doi]
AB  - BACKGROUND: Puerarin shows inhibitory effects on inflammation in chronic heart 
      failure (CHF), but its efficacy in coronary heart disease (CHD) remained vague. 
      METHODS: Rat CHD model was constructed, and serum parameters were determined 
      using a blood liquid biochemical analyzer. Also, contents of creatine kinase 
      (CK), creatine kinase MB isoenzyme (CK-MB), lactate dehydrogenase (LDH) and 
      cardiac troponin (cTnT) were measured using colorimetry. Histological examination 
      was conducted with Hematoxylin-Eosin (H&E) staining, and cardiac function was 
      assessed by Echocardiography. Cell apoptosis was detected using Terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. 
      Relative expressions were measured using quantitative real-time polymerase chain 
      reaction (qRT-PCR) and Western blot as needed. RESULTS: In CHD rats, the levels 
      of TC, LDL and TG and the expressions of matrix metalloproteinase-9 (MMP-9), CD40 
      ligand (CD40L), tumor necrosis factor-alpha (TNF-alpha) and C-reactive protein (CRP) were 
      increased while HDL level was decreased, accompanied with inflammatory cell 
      infiltration and cardiac malfunction. Also, the contents of CK, CK-MB, LDH and 
      cTnT, the percentage of apoptotic cells, the expressions of Bcl-2 associated X 
      protein (Bax), cleaved Caspase-3, TNF-alpha, Interleukin-beta (IL-beta), IL-6 and 
      Lipoprotein-associated Phospholipase A2 (Lp-PLA2) expressions and the levels of 
      oxidized-(ox-)LDL and malondialdehyde (MDA) were upregulated, while the level of 
      super oxidase dismutase (SOD) and the expressions of B cell lymphoma-2 (Bcl-2) 
      and vascular endothelial growth factor (VEGF) were downregulated. However, 
      Puerarin ameliorated the effects of CHD model construction, suppressed nuclear 
      factor-(NF-)kappaB expression, and enhanced the expressions of Farnesoid X Receptor 
      (FXR), phosphorylated-AKT (p-AKT) and phosphorylated-signal transducer and 
      activator of transcription 3 (p-STAT3). CONCLUSION: Puerarin alleviated CHD in 
      rats via inhibiting inflammation, providing possible method for CHD treatment.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhao, Liangping
AU  - Zhao L
AD  - Department of Cardiology, The Second Affiliated Hospital of Soochow University, 
      Gusu District, Suzhou, Jiangsu Province 215004, China. Electronic address: 
      zhlianhping_zhaolp@163.com.
FAU - Wang, Li
AU  - Wang L
AD  - Department of Cardiology, The Second Affiliated Hospital of Soochow University, 
      Gusu District, Suzhou, Jiangsu Province 215004, China.
FAU - Zhang, Daimin
AU  - Zhang D
AD  - Department of Cardiology, Nanjing First Hospital, Qinhuai District, Nanjing, 
      Jiangsu Province 210001, China.
FAU - Chen, Yuqi
AU  - Chen Y
AD  - Department of Cardiology, The Second Affiliated Hospital of Soochow University, 
      Gusu District, Suzhou, Jiangsu Province 215004, China.
FAU - Jin, Fulu
AU  - Jin F
AD  - Department of Cardiology, The Second Affiliated Hospital of Soochow University, 
      Gusu District, Suzhou, Jiangsu Province 215004, China.
LA  - eng
PT  - Journal Article
DEP - 20201215
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Isoflavones)
RN  - 0 (Troponin C)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.3.2 (Creatine Kinase)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*administration & dosage/pharmacology
MH  - Coronary Disease/*drug therapy/genetics
MH  - Creatine Kinase/blood
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/drug effects
MH  - Gene Regulatory Networks/*drug effects
MH  - Isoflavones/*administration & dosage/pharmacology
MH  - L-Lactate Dehydrogenase/blood
MH  - Male
MH  - Rats
MH  - Troponin C/blood
OTO - NOTNLM
OT  - Apoptosis
OT  - Coronary heart disease
OT  - Inflammation
OT  - Puerarin
EDAT- 2020/12/18 06:00
MHDA- 2021/01/22 06:00
CRDT- 2020/12/17 20:11
PHST- 2020/08/24 00:00 [received]
PHST- 2020/10/30 00:00 [revised]
PHST- 2020/12/01 00:00 [accepted]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2021/01/22 06:00 [medline]
PHST- 2020/12/17 20:11 [entrez]
AID - S0378-1119(20)31023-4 [pii]
AID - 10.1016/j.gene.2020.145354 [doi]
PST - ppublish
SO  - Gene. 2021 Mar 1;771:145354. doi: 10.1016/j.gene.2020.145354. Epub 2020 Dec 15.