PMID- 33333415
OWN - NLM
STAT- MEDLINE
DCOM- 20210525
LR  - 20240226
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 90
DP  - 2021 Jan
TI  - Human umbilical cord mesenchymal stem cells-derived exosomal microRNA-17-3p 
      ameliorates inflammatory reaction and antioxidant injury of mice with diabetic 
      retinopathy via targeting STAT1.
PG  - 107010
LID - S1567-5769(20)30621-4 [pii]
LID - 10.1016/j.intimp.2020.107010 [doi]
AB  - BACKGROUND: Accumulating evidence has reported the role of microRNA (miR) on 
      diabetic retinopathy (DR). Thus, the aim of the study was to investigate the 
      effect of exosomal miR-17-3p targeting signal transducer and activator of 
      transcription 1 (STAT1) on inflammatory reaction and antioxidant injury of DR 
      mice. METHODS: A mouse diabetes model was established and injected with 
      miR-17-3p-containing human umbilical cord mesenchymal stem cells 
      (hucMSCs)-derived exosomes to ascertain the role of exosomal miR-17-3p. The blood 
      glucose, glycosylated hemoglobin (HbAlc), weight, hemoglobin (Hb) content, 
      inflammatory factors, oxidative stress factors, vascular endothelial growth 
      factor (VEGF), apoptosis index and glutamine synthetase (GS) level in serum 
      and/or retinal tissues of DR mice were measured. miR-17-3p and STAT1 expression 
      in retinal tissues as well as the target relationship between miR-17-3p and STAT1 
      were tested. RESULTS: miR-17-3p decreased and STAT1 increased in retinal tissues 
      of DR mice, and STAT1 was the target gene of miR-17-3p. Injection of up-regulated 
      exosomal miR-17-3p reduced the blood glucose and HbAlc, increased the weight, Hb 
      content and GS level, decreased contents of inflammatory factors and VEGF, 
      alleviated oxidative injury, and inhibited retinal cell apoptosis in DR mice 
      through inhibiting STAT1. CONCLUSION: Functional studies reveal that 
      hucMSCs-derived exosomes shuffle miR-17-3p to ameliorate inflammatory reaction 
      and oxidative injury of DR mice via targeting STAT1.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Li, Wei
AU  - Li W
AD  - Department of Ophthalmology, The Second Clinical Medical College of Jinan 
      University, Shenzhen People's Hospital, Shenzhen 518000, Guangdong, China.
FAU - Jin, Long-Yu
AU  - Jin LY
AD  - Department of Ophthalmology, The Second Clinical Medical College of Jinan 
      University, Shenzhen People's Hospital, Shenzhen 518000, Guangdong, China.
FAU - Cui, Yu-Bo
AU  - Cui YB
AD  - Department of Ophthalmology, The Second Clinical Medical College of Jinan 
      University, Shenzhen People's Hospital, Shenzhen 518000, Guangdong, China.
FAU - Xie, Ning
AU  - Xie N
AD  - Department of Ophthalmology, The Second Clinical Medical College of Jinan 
      University, Shenzhen People's Hospital, Shenzhen 518000, Guangdong, China. 
      Electronic address: XIEning86u@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201214
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Blood Glucose)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (HbA(1c) protein, mouse)
RN  - 0 (Inflammation Mediators)
RN  - 0 (MIRN17 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (STAT1 Transcription Factor)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Blood Glucose/metabolism
MH  - Cells, Cultured
MH  - Diabetic Retinopathy/genetics/metabolism/pathology/*therapy
MH  - Disease Models, Animal
MH  - Exosomes/genetics/metabolism/*transplantation
MH  - Glycated Hemoglobin/metabolism
MH  - Humans
MH  - Inflammation Mediators/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/genetics/*metabolism
MH  - *Oxidative Stress
MH  - Retina/*metabolism/pathology
MH  - STAT1 Transcription Factor/genetics/*metabolism
MH  - Signal Transduction
MH  - Umbilical Cord/cytology
MH  - Mice
OTO - NOTNLM
OT  - Diabetic retinopathy
OT  - Exosomes
OT  - Signal transducer and activator of transcription 1
OT  - microRNA-17-3p
EDAT- 2020/12/18 06:00
MHDA- 2021/05/26 06:00
CRDT- 2020/12/17 20:14
PHST- 2020/05/09 00:00 [received]
PHST- 2020/09/11 00:00 [revised]
PHST- 2020/09/11 00:00 [accepted]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2021/05/26 06:00 [medline]
PHST- 2020/12/17 20:14 [entrez]
AID - S1567-5769(20)30621-4 [pii]
AID - 10.1016/j.intimp.2020.107010 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2021 Jan;90:107010. doi: 10.1016/j.intimp.2020.107010. Epub 
      2020 Dec 14.