PMID- 33333443
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20220531
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 161
DP  - 2021 Feb
TI  - Alternations in the expression of selected matrix metalloproteinases (MMP-2, -9, 
      -10, and -13) and their tissue inhibitors (TIMP-2 and -3) and MMP-2 and -9 
      activity in the chicken ovary during pause in laying induced by fasting.
PG  - 176-186
LID - S0093-691X(20)30637-3 [pii]
LID - 10.1016/j.theriogenology.2020.12.005 [doi]
AB  - Matrix metalloproteinases (MMPs) are a large group of proteolytic enzymes 
      involved in extracellular matrix turnover in the ovary. Under physiological 
      conditions, the activity of MMPs is controlled by specific tissue inhibitors of 
      MMPs (TIMPs). Information concerning the role and regulation of MMPs in the 
      chicken ovary is scarce. This study was undertaken to examine the expression of 
      selected MMPs and their TIMPs in the chicken ovary during a pause in egg laying 
      induced by feed deprivation. The activities of MMP-2 and MMP-9 were investigated 
      as well. Real-time polymerase chain reaction and Western blot analyses showed 
      changes in the expression of gelatinases (MMP-2, MMP-9), stromelysin (MMP-10), 
      collagenase (MMP-13), TIMP-2, and TIMP-3 on mRNA and/or protein levels in the 
      prehierarchical white (WFs) and yellowish (YFs) follicles, as well as in the 
      largest yellow preovulatory (F3-F1) follicles. In feed-deprived hens, the 
      occurrence of ovarian regression was accompanied by (1) a pronounced decrease in 
      mRNA expression of the examined MMPs and TIMP-3 in all tissues except the YFs 
      where the expression of MMP-13 was higher than in the control hen ovary; (2) an 
      increase in the transcript abundance of TIMP-2 in the yellow atretic follicles; 
      (3) a decrease or no changes in MMP-2 and MMP-9 protein expression in all 
      tissues; (4) an increase in the total activity of gelatinases in the YFs and 
      theca layer of F3; and (5) a decrease in the activity of MMP-2 in F3-F1 follicles 
      and MMP-9 in the theca of F3. In summary, the results of the current study 
      suggest that the selected MMPs and TIMPs may not be involved in the regulation of 
      the advanced stages of atresia of the largest yellow preovulatory follicles in 
      the chicken ovary. This event may require different cell signaling pathways.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Wolak, Dominika
AU  - Wolak D
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, Al. Mickiewicza 24/28, 30-059, Krakow, Poland.
FAU - Hrabia, Anna
AU  - Hrabia A
AD  - Department of Animal Physiology and Endocrinology, University of Agriculture in 
      Krakow, Al. Mickiewicza 24/28, 30-059, Krakow, Poland. Electronic address: 
      anna.hrabia@urk.edu.pl.
LA  - eng
PT  - Journal Article
DEP - 20201209
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - *Fasting
MH  - Female
MH  - Matrix Metalloproteinases/*genetics
MH  - Ovary/*metabolism
MH  - Tissue Inhibitor of Metalloproteinases/*genetics
OTO - NOTNLM
OT  - Chicken
OT  - Fasting
OT  - Follicle atresia
OT  - MMPs
OT  - Ovary
OT  - TIMPs
EDAT- 2020/12/18 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/12/17 20:15
PHST- 2020/07/28 00:00 [received]
PHST- 2020/12/02 00:00 [revised]
PHST- 2020/12/06 00:00 [accepted]
PHST- 2020/12/18 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/12/17 20:15 [entrez]
AID - S0093-691X(20)30637-3 [pii]
AID - 10.1016/j.theriogenology.2020.12.005 [doi]
PST - ppublish
SO  - Theriogenology. 2021 Feb;161:176-186. doi: 10.1016/j.theriogenology.2020.12.005. 
      Epub 2020 Dec 9.