PMID- 33335521
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201219
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Antioxidant and Antiproliferative Activity of Allium ursinum and Their Associated 
      Microbiota During Simulated in vitro Digestion in the Presence of Food Matrix.
PG  - 601616
LID - 10.3389/fmicb.2020.601616 [doi]
LID - 601616
AB  - In this study, for the first time, the comprehensive analysis of 
      antiproliferative and antioxidant activities of ramson, followed by the analysis 
      of its associated microbiota and health-promoting effects of lactic acid bacteria 
      (LAB), was performed. Ramson (Allium ursinum) is recognized as a medicinal plant 
      with a long history of use in traditional medicine due to its antimicrobial and 
      antioxidant activity. In this study the influence of in vitro gastrointestinal 
      digestion on the cytotoxic activity of A. ursinum extracts against human 
      malignant cell lines was demonstrated. Seven sulfur compounds, the degradation 
      products of thiosulfinates, including diallyl disulfide were shown to inhibit 
      proliferation of malignant cells by inducing accumulation within G2/M phase as 
      well as to induce apoptosis through activation of caspase-3 and mitochondrial 
      signaling pathway. Further, the A. ursinum microbiota, particularly LAB with 
      potential probiotic effects, was analyzed by culture-dependent method and 
      culture-independent method [denaturing gradient gel electrophoresis (DGGE)]. The 
      obtained results revealed that the most abundant genera were Streptococcus, 
      Lactobacillus, and Bacillus. The Lactobacillus genus was mainly represented by L. 
      fermentum. The pulsed-field gel electrophoresis (PFGE) analysis revealed the 
      presence of two PFGE pulsotypes. The probiotic potential of the strain L. 
      fermentum BGSR163 belonging to PFGE pulsotype 1 and the strain L. fermentum 
      BGSR227 belonging to the PFGE pulsotype 2 was characterized. The results revealed 
      that both strains are safe for human use, successfully survive the simulated 
      gastrointestinal conditions, have potential to transiently colonize the 
      gastrointestinal tract (GIT) and have a protective immunomodulatory effect, 
      inducing the production of proinflammatory cytokine IL17 and regulatory cytokine 
      IL10, while decreasing the production of proinflammatory cytokine IFN-gamma. In 
      conclusion, the results of this study suggest that consumption of A. ursinum 
      might have health-promoting properties, including anticancer effects, while L. 
      fermentum strains isolated from A. ursinum leaves could be used as probiotics for 
      human consumption.
CI  - Copyright (c) 2020 Stanisavljevic, Sokovic Bajic, Jovanovic, Matic, Tolinacki, 
      Popovic, Popovic, Terzic-Vidojevic, Golic, Beskoski and Samardzic.
FAU - Stanisavljevic, Nemanja
AU  - Stanisavljevic N
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Sokovic Bajic, Svetlana
AU  - Sokovic Bajic S
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Jovanovic, Zivko
AU  - Jovanovic Z
AD  - Faculty of Biology, University of Belgrade, Belgrade, Serbia.
FAU - Matic, Ivana
AU  - Matic I
AD  - Institute of Oncology and Radiology of Serbia, Belgrade, Serbia.
FAU - Tolinacki, Maja
AU  - Tolinacki M
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Popovic, Dusanka
AU  - Popovic D
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Popovic, Nikola
AU  - Popovic N
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Terzic-Vidojevic, Amarela
AU  - Terzic-Vidojevic A
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Golic, Natasa
AU  - Golic N
AD  - Laboratory for Molecular Microbiology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
FAU - Beskoski, Vladimir
AU  - Beskoski V
AD  - Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
FAU - Samardzic, Jelena
AU  - Samardzic J
AD  - Laboratory for Plant Molecular Biology, Institute of Molecular Genetics and 
      Genetic Engineering, University of Belgrade, Belgrade, Serbia.
LA  - eng
PT  - Journal Article
DEP - 20201201
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7736176
OTO - NOTNLM
OT  - antioxidant -phytochemical studies
OT  - antiproliferative
OT  - immunomodulation
OT  - probiotic
OT  - ramson
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2020/12/19 06:00
MHDA- 2020/12/19 06:01
PMCR- 2020/12/01
CRDT- 2020/12/18 05:50
PHST- 2020/09/01 00:00 [received]
PHST- 2020/11/09 00:00 [accepted]
PHST- 2020/12/18 05:50 [entrez]
PHST- 2020/12/19 06:00 [pubmed]
PHST- 2020/12/19 06:01 [medline]
PHST- 2020/12/01 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.601616 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Dec 1;11:601616. doi: 10.3389/fmicb.2020.601616. 
      eCollection 2020.