PMID- 33336000
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201219
IS  - 2391-5463 (Print)
IS  - 2391-5463 (Electronic)
VI  - 15
IP  - 1
DP  - 2020
TI  - miR-101-3p sensitizes non-small cell lung cancer cells to irradiation.
PG  - 413-423
LID - 10.1515/med-2020-0044 [doi]
AB  - Recent studies have revealed that microRNAs regulate radiosensitivity of 
      non-small cell lung cancer (NSCLC). The aim of this study was to investigate 
      whether miR-101-3p is correlated with radiosensitivity of NSCLC. According to our 
      results, miR-101-3p was downregulated in NSCLC tissues and cell lines. Moreover, 
      miR-101-3p was decreased in A549 cells' response to irradiation in a 
      dose-dependent manner. Upregulation of miR-101-3p decreased survival fraction and 
      colony formation rate and increased irradiation-induced apoptosis in 
      irradiation-resistant cells, while miR-101-3p depletion had the opposite effects 
      in irradiation-sensitive cells. Furthermore, mechanistic target of rapamycin 
      (mTOR) is a target gene of miR-101-3p. The expressions of mTOR, p-mTOR, and p-S6 
      were curbed by overexpression of miR-101-3p in A549R cells, which was enhanced by 
      repression of miR-101-3p in A549 cells. Intriguingly, elevation in mTOR abated 
      miR-101-3p upregulation-induced increase in irradiation sensitivity in 
      irradiation-resistant cell line. In contrast, rapamycin undermined miR-101-3p 
      inhibitor-mediated reduction of irradiation sensitivity in irradiation-sensitive 
      cell line. Besides, miR-101-3p overexpression enhanced the efficacy of radiation 
      in an NSCLC xenograft mouse model. In conclusion, miR-101-3p sensitized A549 
      cells to irradiation via inhibition of mTOR-signaling pathway.
CI  - (c) 2020 Zhonghui Li et al., published by De Gruyter.
FAU - Li, Zhonghui
AU  - Li Z
AD  - Department of Oncology, the Third Affiliated Hospital of Inner Mongolia Medical 
      University, Inner Mongolia, China.
FAU - Qu, Zhenjie
AU  - Qu Z
AD  - Department of Oncology, the Third Affiliated Hospital of Inner Mongolia Medical 
      University, Inner Mongolia, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Gerontology, the Third Affiliated Hospital of Inner Mongolia 
      Medical University, Inner Mongolia, China.
FAU - Qin, Meilin
AU  - Qin M
AD  - Department of Oncology, the Third Affiliated Hospital of Inner Mongolia Medical 
      University, Inner Mongolia, China.
FAU - Zhang, Hua
AU  - Zhang H
AD  - Department of Oncology, the Third Affiliated Hospital of Inner Mongolia Medical 
      University, Inner Mongolia, China.
LA  - eng
PT  - Journal Article
DEP - 20200607
PL  - Poland
TA  - Open Med (Wars)
JT  - Open medicine (Warsaw, Poland)
JID - 101672167
PMC - PMC7712473
OTO - NOTNLM
OT  - NSCLC
OT  - irradiation
OT  - mTOR
OT  - miR-101-3p
COIS- Conflicts of interest: There are no conflicts of interest to declare.
EDAT- 2020/12/19 06:00
MHDA- 2020/12/19 06:01
PMCR- 2020/06/07
CRDT- 2020/12/18 05:53
PHST- 2019/04/25 00:00 [received]
PHST- 2019/09/16 00:00 [revised]
PHST- 2019/09/30 00:00 [accepted]
PHST- 2020/12/18 05:53 [entrez]
PHST- 2020/12/19 06:00 [pubmed]
PHST- 2020/12/19 06:01 [medline]
PHST- 2020/06/07 00:00 [pmc-release]
AID - med-2020-0044 [pii]
AID - 10.1515/med-2020-0044 [doi]
PST - epublish
SO  - Open Med (Wars). 2020 Jun 7;15(1):413-423. doi: 10.1515/med-2020-0044. 
      eCollection 2020.