PMID- 33336053
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 2391-5463 (Print)
IS  - 2391-5463 (Electronic)
VI  - 15
IP  - 1
DP  - 2020
TI  - Glial cell induced neural differentiation of bone marrow stromal cells.
PG  - 954-961
LID - 10.1515/med-2020-0229 [doi]
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) have an important application 
      prospect in the field of cell therapy for various neurodegenerative diseases, and 
      inducing factors that regulate BMSC differentiation are proposed as a promising 
      therapeutic strategy. In this study, we explored the effect of glial cell-derived 
      neurotrophic factor (GDNF) on the course of BMSC differentiation. METHODS: BMSCs 
      were isolated from rat bone marrow and induced by GDNF. The effects of GDNF on 
      BMSC viability and proliferation were verified by cell counting kit-8, MTT, 
      bromodeoxyuridine, and flow cytometry assays. Neuronal differentiation from BMSCs 
      was detected by quantitative real-time polymerase chain reaction and 
      immunofluorescence via measuring the expression of several neural specific 
      markers. RESULTS: Compared to untreated BMSCs, GDNF induced the differentiation 
      of BMSCs into neuron-like cells and enhanced the expression levels of neuronal 
      markers including nestin and NCAM. Moreover, the expression of SCF was suppressed 
      by GDNF stimulation. CONCLUSION: GDNF could elevate the differentiation of BMSCs 
      into neuron-like cells and could be considered as an effective candidate cell for 
      future neuroscience research.
CI  - (c) 2020 Qiang Ma et al., published by De Gruyter.
FAU - Ma, Qiang
AU  - Ma Q
AD  - School of Life Science and Biotechnology, Dalian University of Technology, No. 2 
      Linggong Road, Dalian 116023, Liaoning Province, China.
AD  - Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian 116001, Liaoning Province, China.
FAU - Cai, Ming
AU  - Cai M
AD  - Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian 116001, Liaoning Province, China.
FAU - Shang, Jing-Wei
AU  - Shang JW
AD  - Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian 116001, Liaoning Province, China.
FAU - Yang, Jun
AU  - Yang J
AD  - School of Life Science and Biotechnology, Dalian University of Technology, No. 2 
      Linggong Road, Dalian 116023, Liaoning Province, China.
FAU - Gu, Xin-Yi
AU  - Gu XY
AD  - Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian 116001, Liaoning Province, China.
FAU - Liu, Wen-Bo
AU  - Liu WB
AD  - Department of Neurology, Affiliated Zhongshan Hospital of Dalian University, 
      Dalian 116001, Liaoning Province, China.
FAU - Yang, Qing
AU  - Yang Q
AUID- ORCID: 0000-0002-4789-430X
AD  - School of Life Science and Biotechnology, Dalian University of Technology, No. 2 
      Linggong Road, Dalian 116023, Liaoning Province, China.
LA  - eng
PT  - Journal Article
DEP - 20200930
PL  - Poland
TA  - Open Med (Wars)
JT  - Open medicine (Warsaw, Poland)
JID - 101672167
PMC - PMC7712328
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - bone marrow stromal cells
OT  - cell therapy
OT  - glial cell-derived neurotrophic factor
OT  - neuronal differentiation
COIS- Conflict of interest: None.
EDAT- 2020/12/19 06:00
MHDA- 2020/12/19 06:01
PMCR- 2020/09/30
CRDT- 2020/12/18 05:53
PHST- 2020/02/13 00:00 [received]
PHST- 2020/08/23 00:00 [revised]
PHST- 2020/08/25 00:00 [accepted]
PHST- 2020/12/18 05:53 [entrez]
PHST- 2020/12/19 06:00 [pubmed]
PHST- 2020/12/19 06:01 [medline]
PHST- 2020/09/30 00:00 [pmc-release]
AID - med-2020-0229 [pii]
AID - 10.1515/med-2020-0229 [doi]
PST - epublish
SO  - Open Med (Wars). 2020 Sep 30;15(1):954-961. doi: 10.1515/med-2020-0229. 
      eCollection 2020.