PMID- 33336750
OWN - NLM
STAT- MEDLINE
DCOM- 20210629
LR  - 20210629
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 23
DP  - 2020 Dec
TI  - MiR-204 reduces apoptosis in rats with myocardial infarction by targeting 
      SIRT1/p53 signaling pathway.
PG  - 12306-12314
LID - 24023 [pii]
LID - 10.26355/eurrev_202012_24023 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the influence of micro 
      ribonucleic acid (miR)-204 on rats with myocardial infarction by targeting the 
      silent information regulator 1 (SIRT1)/p53 signaling pathway. MATERIALS AND 
      METHODS: A total of 36 Sprague-Dawley rats were randomly divided into three 
      groups, including: sham-operation group (n=12), model group (n=12) and miR-204 
      mimics group (n=12). The rats in the sham-operation group only underwent 
      thoracotomy, without myocardial infarction injury. Meanwhile, the rats in model 
      group and miR-204 mimics group were utilized to establish the models of 
      myocardial infarction, and then, intervened with normal saline and miR-204 
      mimics, respectively. The morphology of myocardial tissues was observed via 
      hematoxylin-eosin (HE) staining. Immunofluorescence was performed to detect the 
      expression of Caspase-3. Target genes of miR-204 were analyzed using bioanalysis 
      software. Western blotting (WB) assay was applied to measure the relative protein 
      expression of SIRT1. MiR-204 expression and the messenger RNA (mRNA) expressions 
      of SIRT1 and p53 were measured via quantitative Polymerase Chain Reaction (qPCR). 
      Furthermore, cell apoptosis was determined through terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay. RESULTS: HE staining 
      showed that the morphology of myocardial tissues was normal in sham-operation 
      group. Severe myocardial tissue injury was visible in model group, and the injury 
      was relieved in miR-204 mimics group when compared with model group. The results 
      manifested that the positive expression of Caspase-3 in cardiac tissues increased 
      remarkably in the model group and miR-204 mimics group in comparison with 
      sham-operation group (p<0.05). Meanwhile, it was evidently lower in miR-204 
      mimics group than model group (p<0.05). Based on the analysis via bioanalysis 
      software, SIRT1 was the target gene of miR-204. WB results revealed that the 
      relative protein expression level of SIRT1 was elevated notably in the other two 
      groups compared with the 2sham-operation group (p<0.05). However, it was markedly 
      lowered in miR-204 mimics group in contrast with model group (p<0.05). QRT-PCR 
      results demonstrated that the model group and miR-204 mimics group exhibited 
      distinctly lower expression of miR-204 but higher mRNA expressions of SIRT1 and 
      p53 than sham-operation group (p<0.05). However, miR-204 mimics group exhibited 
      prominently higher expression of miR-204 but lower mRNA expressions of SIRT1 and 
      p53 than model group (p<0.05). Finally, the results of TUNEL assay demonstrated 
      that the apoptosis rate increased remarkably in the model group and miR-204 
      mimics group when compared with sham-operation group (p<0.05). However, it 
      decreased notably in miR-204 mimics group in comparison with model group 
      (p<0.05). CONCLUSIONS: MiR-204 reduces the apoptosis level in rats with 
      myocardial infarction via targeted inhibition of the SIRT1/p53 signaling pathway.
FAU - Wang, L-Z
AU  - Wang LZ
AD  - Department of Cardiac, Beijing Rehabilitation Hospital, Capital Medical 
      University, Beijing, China. urologistliu@hotmail.com.
FAU - Xi, J-N
AU  - Xi JN
FAU - Liu, T-J
AU  - Liu TJ
FAU - Zhang, Z-Y
AU  - Zhang ZY
FAU - Zhang, P
AU  - Zhang P
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN204 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Tp53 protein, rat)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - MicroRNAs/genetics/*metabolism
MH  - Myocardial Infarction/*metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - Sirtuin 1/genetics/*metabolism
MH  - Tumor Suppressor Protein p53/genetics/*metabolism
EDAT- 2020/12/19 06:00
MHDA- 2021/06/30 06:00
CRDT- 2020/12/18 08:37
PHST- 2020/12/18 08:37 [entrez]
PHST- 2020/12/19 06:00 [pubmed]
PHST- 2021/06/30 06:00 [medline]
AID - 24023 [pii]
AID - 10.26355/eurrev_202012_24023 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12306-12314. doi: 
      10.26355/eurrev_202012_24023.