PMID- 33339862
OWN - NLM
STAT- MEDLINE
DCOM- 20210428
LR  - 20210816
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Dec 18
TI  - Methylation of estrogen receptor 2 (ESR2) in deep paravertebral muscles and its 
      association with idiopathic scoliosis.
PG  - 22331
LID - 10.1038/s41598-020-78454-4 [doi]
LID - 22331
AB  - Idiopathic scoliosis (IS) is one of the most common spinal disorders in 
      adolescents. Despite many studies, the etiopathogenesis of IS is still poorly 
      understood. In recent years, the role of epigenetic factors in the 
      etiopathogenesis of IS has been increasingly investigated. It has also been 
      postulated that the development and progression of the disease is related to 
      gender and puberty, and could be associated with estrogen action. Estrogen 
      hormones act via estrogen receptor 1 (ESR1) and estrogen receptor 2 (ESR2). It 
      has been suggested that ESR2 expression is dependent on methylation within its 
      gene promoter. So far, no studies have evaluated local, tissue-specific DNA 
      methylation in patients with IS. Thus, our study aimed to analyze the methylation 
      and expression level of ESR2 in the paraspinal muscles of the convex and concave 
      side of the IS curvature. The methylation level within ESR2 promoter 0N, but not 
      exon 0N, was significantly higher on the concave side of the curvature compared 
      to the convex side. There was no significant correlation between ESR2 expression 
      and methylation level in the promoter 0N on the convexity of thoracic scoliosis, 
      whereas, on the concave side of the curvature, we observed a moderate negative 
      correlation. There was no difference in the methylation levels of the ESR2 
      promoter and exon 0N between groups of patients with Cobb angle </= 70 degrees  and > 70 degrees  
      on the concave and convex side of the curvature. We also found no statistically 
      significant correlation between the Cobb angle value and the mean methylation 
      level in either the ESR2 promoter or exon 0N on the convex or concave side of the 
      curvature. Our findings demonstrate that DNA methylation at the ESR2 promoter in 
      deep paravertebral muscle tissue is associated with the occurrence but not with 
      the severity of idiopathic scoliosis.
FAU - Chmielewska, Malgorzata
AU  - Chmielewska M
AUID- ORCID: 0000-0003-0091-6492
AD  - Chair and Department of Cell Biology, Poznan University of Medical Sciences, 
      Rokietnicka Street 5D, Poznan, Poland. mchmielewska@ump.edu.pl.
FAU - Janusz, Piotr
AU  - Janusz P
AUID- ORCID: 0000-0001-5702-4933
AD  - Department of Spine Disorders and Pediatric Orthopedics, Poznan University of 
      Medical Sciences, 28 Czerwca 1956 r. Street 135/147, Poznan, Poland.
FAU - Andrusiewicz, Miroslaw
AU  - Andrusiewicz M
AUID- ORCID: 0000-0002-8781-3447
AD  - Chair and Department of Cell Biology, Poznan University of Medical Sciences, 
      Rokietnicka Street 5D, Poznan, Poland.
FAU - Kotwicki, Tomasz
AU  - Kotwicki T
AUID- ORCID: 0000-0003-0810-9361
AD  - Department of Spine Disorders and Pediatric Orthopedics, Poznan University of 
      Medical Sciences, 28 Czerwca 1956 r. Street 135/147, Poznan, Poland.
FAU - Kotwicka, Malgorzata
AU  - Kotwicka M
AUID- ORCID: 0000-0002-9802-374X
AD  - Chair and Department of Cell Biology, Poznan University of Medical Sciences, 
      Rokietnicka Street 5D, Poznan, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201218
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (ESR1 protein, human)
RN  - 0 (ESR2 protein, human)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
SB  - IM
MH  - Adolescent
MH  - DNA Methylation/*genetics
MH  - Estrogen Receptor alpha/*genetics
MH  - Estrogen Receptor beta/*genetics
MH  - Estrogens/genetics
MH  - Female
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Male
MH  - Muscle, Skeletal/metabolism/pathology
MH  - Organ Specificity/genetics
MH  - Paraspinal Muscles
MH  - Promoter Regions, Genetic/genetics
MH  - Scoliosis/*genetics/physiopathology
PMC - PMC7749113
COIS- The authors declare no competing interests.
EDAT- 2020/12/20 06:00
MHDA- 2021/04/29 06:00
PMCR- 2020/12/18
CRDT- 2020/12/19 05:26
PHST- 2020/07/14 00:00 [received]
PHST- 2020/11/25 00:00 [accepted]
PHST- 2020/12/19 05:26 [entrez]
PHST- 2020/12/20 06:00 [pubmed]
PHST- 2021/04/29 06:00 [medline]
PHST- 2020/12/18 00:00 [pmc-release]
AID - 10.1038/s41598-020-78454-4 [pii]
AID - 78454 [pii]
AID - 10.1038/s41598-020-78454-4 [doi]
PST - epublish
SO  - Sci Rep. 2020 Dec 18;10(1):22331. doi: 10.1038/s41598-020-78454-4.