PMID- 33341705
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20231213
IS  - 1532-8198 (Electronic)
IS  - 1092-9134 (Linking)
VI  - 50
DP  - 2021 Feb
TI  - Diagnostic application of cyclin D1 fluorescent in situ hybridization for 
      histologically undetermined early lesions of acral melanoma in situ: A case 
      series.
PG  - 151681
LID - S1092-9134(20)30227-6 [pii]
LID - 10.1016/j.anndiagpath.2020.151681 [doi]
AB  - Histologically undetermined early acral melanoma in situ (HUAMIS) is rare but a 
      diagnostic challenge, being clinically and dermoscopically MIS (late onset, a 
      large size (>7 mm), parallel ridges pattern) but microscopically without 
      recognizable cytological atypia. Cyclin D1 (CCND1) gene amplification is a 
      genetic aberration occurring in the early radial growth phase of AMs and could 
      thus help determine malignancy for this disease. We determine the value of CCND1 
      amplification by FISH as a diagnostic marker for HUAMIS. CCND1 amplification was 
      examined in paraffin-embedded skin biopsies and excisions using a dual-probes 
      fluorescence in situ hybridization (FISH) (11q13 and CEP11). One FISH-negative 
      case 6 was additionally examined by Mypath Melanoma (qRT-PCR). Seventeen cases 
      (12 dysplastic nevi, 3 AMIS, and 2 invasive AM) were served as negative controls 
      for FISH. All six patients (4 females and 2 males) were Hispanic. Pigment lesions 
      were on the left plantar foot (4), right third finger palm (1), and right thumb 
      subungual (1). All cases showed similar clinical and dermoscopical 
      characteristics, including late onset (50 to 74 years old), long duration (from 2 
      to 15 years), large-sized pigments (from 16 to 40 mm), and a parallel ridge 
      pattern. Junctional melanocytes with no or minimal atypia from five cases showed 
      CCND1 amplifications. Four of 5 cases were received 1st or/and 2nd wide 
      excisions, which demonstrated foci of histologically overt MIS. One FISH-negative 
      case 6 demonstrated "likely malignancy" scores (>2) by Mypath Melanoma (qRT-PCR). 
      None of negative controls showed the amplification. We propose here a simple 
      CCND1 FISH is a practical diagnostic test to determine the malignancy of the very 
      early progression phase of AM preceding histopathologically defined MIS. Cases 
      presented here could be an indolent subtype of AMIS characterized by carrying a 
      long latent radial growth phase without vertical growth, mimicking lentigo 
      maligna.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Cho-Vega, Jeong Hee
AU  - Cho-Vega JH
AD  - Departments of Pathology, Miller School of Medicine, University of Miami, 
      Florida, USA. Electronic address: vegaelena@ymail.com.
FAU - Cao, Theresa
AU  - Cao T
AD  - Dr. Philip Frost Department of Dermatology and Cutaneous Surgery, Miller School 
      of Medicine, University of Miami, Florida, USA.
FAU - Ledon, Jennifer
AU  - Ledon J
AD  - Dr. Philip Frost Department of Dermatology and Cutaneous Surgery, Miller School 
      of Medicine, University of Miami, Florida, USA.
FAU - Moller, Mecker
AU  - Moller M
AD  - Surgical Oncology, Sylvester Comprehensive Cancer Center, Miller School of 
      Medicine, University of Miami, Florida, USA.
FAU - Avisar, Eli
AU  - Avisar E
AD  - Surgical Oncology, Sylvester Comprehensive Cancer Center, Miller School of 
      Medicine, University of Miami, Florida, USA.
FAU - Elgart, George
AU  - Elgart G
AD  - Dr. Philip Frost Department of Dermatology and Cutaneous Surgery, Miller School 
      of Medicine, University of Miami, Florida, USA.
FAU - Tan, Jing Hong
AU  - Tan JH
AD  - Molecular Pathology, Miller School of Medicine, University of Miami, Florida, 
      USA.
FAU - Fan, Yao-Shan
AU  - Fan YS
AD  - Molecular Pathology, Miller School of Medicine, University of Miami, Florida, 
      USA.
FAU - Grichnik, James M
AU  - Grichnik JM
AD  - Dr. Philip Frost Department of Dermatology and Cutaneous Surgery, Miller School 
      of Medicine, University of Miami, Florida, USA.
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20201213
PL  - United States
TA  - Ann Diagn Pathol
JT  - Annals of diagnostic pathology
JID - 9800503
RN  - 0 (CCND1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - Biopsy
MH  - Cyclin D1/*metabolism
MH  - Dermoscopy/methods
MH  - Female
MH  - Follow-Up Studies
MH  - Gene Amplification/genetics
MH  - Hispanic or Latino/genetics/statistics & numerical data
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Melanocytes/*metabolism/pathology
MH  - Melanoma/diagnosis/*metabolism/pathology/surgery
MH  - Middle Aged
MH  - Skin/pathology
MH  - Skin Neoplasms/diagnosis/*metabolism/pathology/surgery
MH  - Treatment Outcome
MH  - Melanoma, Cutaneous Malignant
OTO - NOTNLM
OT  - Cyclin D1 amplification
OT  - Fluorescence in situ hybridization
OT  - Histologically undetermined acral melanoma in situ
EDAT- 2020/12/21 06:00
MHDA- 2021/10/21 06:00
CRDT- 2020/12/20 20:29
PHST- 2020/12/07 00:00 [received]
PHST- 2020/12/09 00:00 [accepted]
PHST- 2020/12/21 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2020/12/20 20:29 [entrez]
AID - S1092-9134(20)30227-6 [pii]
AID - 10.1016/j.anndiagpath.2020.151681 [doi]
PST - ppublish
SO  - Ann Diagn Pathol. 2021 Feb;50:151681. doi: 10.1016/j.anndiagpath.2020.151681. 
      Epub 2020 Dec 13.