PMID- 33342265
OWN - NLM
STAT- MEDLINE
DCOM- 20211112
LR  - 20220531
IS  - 0031-949X (Print)
IS  - 0031-949X (Linking)
VI  - 111
IP  - 8
DP  - 2021 Aug
TI  - NBS-LRR Gene TaRPS2 is Positively Associated with the High-Temperature Seedling 
      Plant Resistance of Wheat Against Puccinia striiformis f. sp. tritici.
PG  - 1449-1458
LID - 10.1094/PHYTO-03-20-0063-R [doi]
AB  - Xiaoyan6 (XY6) is a wheat (Triticum aestivum) cultivar possessing 
      nonrace-specific high-temperature seedling plant (HTSP) resistance against stripe 
      rust, caused by Puccinia striiformis f. sp. tritici. Previously, we identified 
      one particular gene, TaRPS2, for its involvement in the HTSP resistance. To 
      elucidate the role of TaRPS2 in the HTSP resistance, we cloned the full length of 
      TaRPS2 from XY6. The transcriptional expression of TaRPS2 was rapidly upregulated 
      (19.11-fold) under the normal-high-normal temperature treatment that induces the 
      HTSP resistance. The expression level of TaRPS2 in leaves was higher than that in 
      the stems and roots. Quantification of the endogenous hormones in wheat leaves 
      after P. striiformis f. sp. tritici inoculation showed that 
      1-aminocyclopropane-1-carboxylic acid, salicylic acid (SA), and jasmonic acid 
      were involved in the HTSP resistance. In addition, detection of hydrogen peroxide 
      (H(2)O(2)) accumulation indicated that reactive oxygen species burst was also 
      associated with the HTSP resistance. Two hours after exogenous H(2)O(2) treatment 
      or 0.5 h after SA treatment, the expression level of TaRPS2 was increased by 2.66 
      and 2.35 times, respectively. The subcellular localization of enhanced green 
      fluorescent protein-TaRPS2 fusion protein was in the nuclei and plasma membranes. 
      Virus-induced gene silencing of TaRPS2 reduced the level of HTSP resistance in 
      XY6. Compared with the nonsilenced leaves, the TaRPS2-silenced leaves had the 
      reduction of necrotic cells but a greater number of uredinia. These results 
      indicated that TaRPS2 positively regulates the HTSP resistance of XY6 against P. 
      striiformis f. sp. tritici and is related to the SA and H(2)O(2) signaling 
      pathways.
FAU - Hu, Yangshan
AU  - Hu Y
AUID- ORCID: 0000-0003-3651-4540
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Tao, Fei
AU  - Tao F
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
AD  - Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, 
      College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 
      730070, China.
FAU - Su, Chang
AU  - Su C
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Li, Juan
AU  - Li J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Wang, Jiahui
AU  - Wang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Xu, Xiangming
AU  - Xu X
AUID- ORCID: 0000-0002-4567-7117
AD  - Pest & Pathogen Ecology, NIAB EMR, East Malling, West Malling, Kent ME19 6BJ, 
      U.K.
FAU - Chen, Xianming
AU  - Chen X
AUID- ORCID: 0000-0002-9013-5974
AD  - Agricultural Research Service, United States Department of Agriculture and 
      Department of Plant Pathology, Washington State University, Pullman, WA 
      99164-6430, U.S.A.
FAU - Shang, Hongsheng
AU  - Shang H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
FAU - Hu, Xiaoping
AU  - Hu X
AUID- ORCID: 0000-0002-8155-7040
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
LA  - eng
PT  - Journal Article
DEP - 20210916
PL  - United States
TA  - Phytopathology
JT  - Phytopathology
JID - 9427222
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Disease Resistance/*genetics
MH  - *Genes, Plant
MH  - Hydrogen Peroxide
MH  - Plant Diseases/*genetics/microbiology
MH  - Puccinia/*pathogenicity
MH  - Seedlings/genetics/microbiology
MH  - Temperature
MH  - *Triticum/genetics/microbiology
OTO - NOTNLM
OT  - H2O2 accumulation
OT  - NBS-LRR
OT  - TaRPS2
OT  - endogenous hormones
OT  - high-temperature seedling plant resistance
OT  - stripe rust
OT  - virus-induced gene silencing
OT  - wheat
EDAT- 2020/12/22 06:00
MHDA- 2021/11/16 06:00
CRDT- 2020/12/21 05:30
PHST- 2020/12/22 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2020/12/21 05:30 [entrez]
AID - 10.1094/PHYTO-03-20-0063-R [doi]
PST - ppublish
SO  - Phytopathology. 2021 Aug;111(8):1449-1458. doi: 10.1094/PHYTO-03-20-0063-R. Epub 
      2021 Sep 16.