PMID- 33343393 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201222 IS - 1664-042X (Print) IS - 1664-042X (Electronic) IS - 1664-042X (Linking) VI - 11 DP - 2020 TI - Evidence for Training-Induced Changes in miRNA Levels in the Skeletal Muscle of Patients With Type 2 Diabetes Mellitus. PG - 599651 LID - 10.3389/fphys.2020.599651 [doi] LID - 599651 AB - Physical training can improve glycemic control in patients with type 2 diabetes mellitus (T2DM). However, the underlying mechanisms are not entirely clear. An interesting piece of the puzzle could be the regulation of micro-RNAs (miRNAs). They are important modulators of protein expression. Some miRNAs were found to be both linked to poor glycemic control/insulin resistance (with evidence from in vivo and/or in vitro studies) and dysregulated in the skeletal muscle of T2DM patients. This pilot study examines whether a 3-month endurance training program [three times a week, 70-80% peak heart rate (HR(peak))] can down-regulate their levels in T2DM men (n = 7). One skeletal muscle biopsy sample was obtained from each patient at T1 (6 weeks pre-intervention), one at T2 (1 week pre-intervention) and one at T3 (3-4 days post-intervention). miRNA-27a-3p, -29a-3p, -29b-3p, -29c-3p, -106b-5p, -135a-5p, -143-3p, -144-3p, -194-5p, and - 206 levels were determined by RT-qPCR. Friedman ANOVA and post-hoc tests showed that miRNA-29b-3p, -29c-3p and -135a-5p levels were significantly reduced post-training (T3 vs. T2 and/or T1). Glycated hemoglobin (HbA1c) and HOMA insulin resistance index did not change significantly. However, HbA1c was reduced in 6 of 7 patients post-training. Furthermore, Spearman's rank correlation analyses with all values from all time points showed significant negative associations between miRNA-29c-3p, -106b-5p, -144-3p and -194-5p levels and cardiorespiratory fitness (VO(2peak)). The study results imply that regular exercise and improving one's physical fitness is helpful for the regulation of skeletal muscle miRNAs in T2DM patients. Whether or not changes in the miRNA profile can affect the clinical situation of T2DM patients warrants further research. CI - Copyright (c) 2020 Simaitis, Schulte-Korne, Schiffer, Bloch, Predel, Brixius and Brinkmann. FAU - Simaitis, Sarah AU - Simaitis S AD - Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany. FAU - Schulte-Korne, Benedikt AU - Schulte-Korne B AD - Department of Preventive and Rehabilitative Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany. FAU - Schiffer, Thorsten AU - Schiffer T AD - Outpatient Clinic for Sports Traumatology and Public Health Consultation, German Sport University Cologne, Cologne, Germany. FAU - Bloch, Wilhelm AU - Bloch W AD - Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany. FAU - Predel, Hans-Georg AU - Predel HG AD - Department of Preventive and Rehabilitative Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany. FAU - Brixius, Klara AU - Brixius K AD - Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany. FAU - Brinkmann, Christian AU - Brinkmann C AD - Department of Preventive and Rehabilitative Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany. AD - IST University of Applied Sciences, Dusseldorf, Germany. LA - eng PT - Journal Article DEP - 20201203 PL - Switzerland TA - Front Physiol JT - Frontiers in physiology JID - 101549006 PMC - PMC7744661 OTO - NOTNLM OT - diabetes OT - epigenetics OT - exercise OT - glycemic control (HbA1c) OT - miRNA - microRNA OT - training COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2020/12/22 06:00 MHDA- 2020/12/22 06:01 PMCR- 2020/12/03 CRDT- 2020/12/21 06:04 PHST- 2020/08/27 00:00 [received] PHST- 2020/11/05 00:00 [accepted] PHST- 2020/12/21 06:04 [entrez] PHST- 2020/12/22 06:00 [pubmed] PHST- 2020/12/22 06:01 [medline] PHST- 2020/12/03 00:00 [pmc-release] AID - 10.3389/fphys.2020.599651 [doi] PST - epublish SO - Front Physiol. 2020 Dec 3;11:599651. doi: 10.3389/fphys.2020.599651. eCollection 2020.