PMID- 33343522
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201222
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Chlamydia psittaci Plasmid-Encoded CPSIT_P7 Elicits Inflammatory Response in 
      Human Monocytes via TLR4/Mal/MyD88/NF-kappaB Signaling Pathway.
PG  - 578009
LID - 10.3389/fmicb.2020.578009 [doi]
LID - 578009
AB  - The chlamydial plasmid, an essential virulence factor, encodes plasmid proteins 
      that play important roles in chlamydial infection and the corresponding immune 
      response. However, the virulence factors and the molecular mechanisms of 
      Chlamydia psittaci are not well understood. In the present study, we investigated 
      the roles and mechanisms of the plasmid-encoded protein CPSIT_P7 of C. psittaci 
      in regulating the inflammatory response in THP-1 cells (human monocytic leukemia 
      cell line). Based on cytokine arrays, CPSIT_P7 induces the expression of 
      interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant 
      protein-1 (MCP-1) in THP-1 cells. Moreover, the expression levels of IL-6, IL-8, 
      and MCP-1 stimulated by CPSIT_P7 declined after silencing of the Toll-like 
      receptor 4 (TLR4) gene using small interfering RNA and transfection of a dominant 
      negative plasmid encoding TLR4 (pZERO-hTLR4). We further demonstrated that 
      transfection with the dominant negative plasmid encoding MyD88 (pDeNy-hMyD88) and 
      the dominant negative plasmid encoding Mal (pDeNy-hMal) could also abrogate the 
      expression of the corresponding proteins. Western blot and immunofluorescence 
      assay results showed that CPSIT_P7 could activate nuclear factor kappaB (NF-kappaB) 
      signaling pathways in THP-1 cells. Altogether, our results indicate that the 
      CPSIT_P7 induces the TLR4/Mal/MyD88/NF-kappaB signaling axis and therefore 
      contributes to the inflammatory cytokine response.
CI  - Copyright (c) 2020 Chen, Li, Yan, Sun, Wang, Liu, Xiao, Lu and Wu.
FAU - Chen, Qian
AU  - Chen Q
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
AD  - Institute of Clinical Research, The First Affiliated Hospital of University of 
      South China, Hengyang, China.
FAU - Li, Yumeng
AU  - Li Y
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
AD  - Department of Clinical Laboratory, The First Affiliated Hospital of University of 
      South China, Hengyang, China.
FAU - Yan, Xiaoliang
AU  - Yan X
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
FAU - Sun, Zhenjie
AU  - Sun Z
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
FAU - Wang, Chuan
AU  - Wang C
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
FAU - Liu, Shuangquan
AU  - Liu S
AD  - Department of Clinical Laboratory, The First Affiliated Hospital of University of 
      South China, Hengyang, China.
FAU - Xiao, Jian
AU  - Xiao J
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
FAU - Lu, Chunxue
AU  - Lu C
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
FAU - Wu, Yimou
AU  - Wu Y
AD  - Institution of Pathogenic Biology, Hengyang Medical College, University of South 
      China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and 
      Control, Hunan Province Cooperative Innovation Center for Molecular Target New 
      Drug Study, University of South China, Hengyang, China.
LA  - eng
PT  - Journal Article
DEP - 20201203
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7744487
OTO - NOTNLM
OT  - CPSIT_P7
OT  - Chlamydia psittaci
OT  - TLR4
OT  - human monocytes
OT  - inflammation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2020/12/22 06:00
MHDA- 2020/12/22 06:01
PMCR- 2020/12/03
CRDT- 2020/12/21 06:04
PHST- 2020/07/19 00:00 [received]
PHST- 2020/10/29 00:00 [accepted]
PHST- 2020/12/21 06:04 [entrez]
PHST- 2020/12/22 06:00 [pubmed]
PHST- 2020/12/22 06:01 [medline]
PHST- 2020/12/03 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.578009 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Dec 3;11:578009. doi: 10.3389/fmicb.2020.578009. 
      eCollection 2020.