PMID- 33344146
OWN - NLM
STAT- MEDLINE
DCOM- 20210826
LR  - 20210826
IS  - 2210-7185 (Electronic)
IS  - 2210-7177 (Print)
IS  - 2210-7177 (Linking)
VI  - 2020
DP  - 2020
TI  - ABCG1 Attenuates Oxidative Stress Induced by H(2)O(2) through the Inhibition of 
      NADPH Oxidase and the Upregulation of Nrf2-Mediated Antioxidant Defense in 
      Endothelial Cells.
PG  - 2095645
LID - 10.1155/2020/2095645 [doi]
LID - 2095645
AB  - Summary. Oxidative stress is an important factor that is related to endothelial 
      dysfunction. ATP-binding cassette transporter G1 (ABCG1), a regulator of 
      intracellular cholesterol efflux, has been found to prevent endothelial 
      activation in vessel walls. To explore the role of ABCG1 in oxidative stress 
      production in endothelial cells, HUAECs were exposed to H(2)O(2) and transfected 
      with the specific ABCG1 siRNA or ABCG1 overexpression plasmid. The results showed 
      that overexpression of ABCG1 by ABCG1 plasmid or liver X receptor (LXR) agonist 
      T0901317 treatment inhibited ROS production and MDA content induced by H(2)O(2) 
      in HUAECs. Furthermore, ABCG1 upregulation blunted the activity of prooxidant 
      NADPH oxidase and the expression of Nox4, one of the NADPH oxidase subunits. 
      Moreover, the increased migration of Nrf2 from the cytoplasm to the nucleus and 
      antioxidant HO-1 expression were detected in HUAECs with upregulation of ABCG1. 
      Conversely, ABCG1 downregulation by ABCG1 siRNA increased NADPH oxidase activity 
      and Nox4 expression and abrogated the increase at Nrf2 nuclear protein levels. In 
      addition, intracellular cholesterol load interfered with the balance between 
      NADPH oxidase activity and HO-1 expression. It was suggested that ABCG1 
      attenuated oxidative stress induced by H(2)O(2) in endothelial cells, which might 
      be involved in the balance between decreased NADPH oxidase activity and increased 
      Nrf2/OH-1 antioxidant defense signaling via its regulation for intracellular 
      cholesterol accumulation.
CI  - Copyright (c) 2020 Jiahong Xue et al.
FAU - Xue, Jiahong
AU  - Xue J
AUID- ORCID: 0000-0003-1079-4767
AD  - Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an 
      Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.
FAU - Fan, Jiali
AU  - Fan J
AD  - Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an 
      Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.
FAU - Li, Yuan
AU  - Li Y
AD  - Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an 
      Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.
FAU - Wu, Wenhuan
AU  - Wu W
AD  - Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an 
      Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.
FAU - Yan, Qing
AU  - Yan Q
AD  - Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an 
      Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.
FAU - Zheng, Qiangsun
AU  - Zheng Q
AUID- ORCID: 0000-0003-2344-4627
AD  - Department of Cardiovascular Medicine, Second Affiliated Hospital of Xi'an 
      Jiaotong University, 157 West Five Road, Xi'an, Shaanxi 710004, China.
LA  - eng
PT  - Journal Article
DEP - 20201203
PL  - United States
TA  - Anal Cell Pathol (Amst)
JT  - Analytical cellular pathology (Amsterdam)
JID - 101541993
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Protein Subunits)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.6.3.- (NADPH Oxidases)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1/*metabolism
MH  - Antioxidants/*metabolism
MH  - Cholesterol/metabolism
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Heme Oxygenase-1/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/*toxicity
MH  - NADPH Oxidases/*metabolism
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Oxidation-Reduction/drug effects
MH  - *Oxidative Stress/drug effects
MH  - Protein Subunits/metabolism
MH  - Umbilical Arteries/pathology
MH  - *Up-Regulation/drug effects
PMC - PMC7732382
COIS- There are no conflicts to declare for this submission.
EDAT- 2020/12/22 06:00
MHDA- 2021/08/27 06:00
PMCR- 2020/12/03
CRDT- 2020/12/21 06:07
PHST- 2020/07/13 00:00 [received]
PHST- 2020/10/23 00:00 [accepted]
PHST- 2020/12/21 06:07 [entrez]
PHST- 2020/12/22 06:00 [pubmed]
PHST- 2021/08/27 06:00 [medline]
PHST- 2020/12/03 00:00 [pmc-release]
AID - 10.1155/2020/2095645 [doi]
PST - epublish
SO  - Anal Cell Pathol (Amst). 2020 Dec 3;2020:2095645. doi: 10.1155/2020/2095645. 
      eCollection 2020.