PMID- 33344811
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201222
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 5
IP  - 49
DP  - 2020 Dec 15
TI  - Rapid and Simultaneous Analysis of Multiple Classes of Antimicrobial Drugs by 
      Liquid Chromatography-Tandem Mass Spectrometry and Its Application to Routine 
      Biomedical, Food, and Soil Analyses.
PG  - 31584-31597
LID - 10.1021/acsomega.0c03863 [doi]
AB  - Antimicrobial agents (AMAs) are widely exploited nowadays to meet the high demand 
      for animal-derived food. It has a significant impact on the food chain whose end 
      consumers are human beings. The burden of AMAs on humans comes from either meat 
      or crops cultivated on soil containing high residual antibiotics, which are 
      responsible for the global crisis of antibiotic resistance. Thus, the objective 
      of this study was to design a selective and sensitive liquid chromatography-mass 
      spectrometry (LC-MS)/MS-based simultaneous bioanalytical method for estimation of 
      twenty AMAs in human plasma, raw meat, and soil samples. The selective extraction 
      of all analytes from the above matrices was performed by the solid-phase 
      extraction clean-up method to overcome the interferences. Analytes were separated 
      on a Waters Symmetry Shield C18 (150 x 4.6 mm(2), 5 mum) column, using an 
      isocratic solvent system of methanol-0.5% formic acid (80:20, v/v) with 0.75 
      mL/min flow rate. The average extraction recoveries for all analytes in plasma 
      were ranged from 42.0 to 94.0% with relative standard deviations (RSDs) below 
      +/-15%. All of the validation parameters are in accordance with the United State 
      Food and Drug Administration (USFDA) guidelines. Moreover, the method was also 
      valid for a broad plasma concentration range and can be proposed as an excellent 
      method for routine pharmacokinetic studies, therapeutic drug monitoring, clinical 
      analysis, and detection and quantitation of AMA remnants in raw meat as a 
      standard quality control test for human consumption.
CI  - (c) 2020 American Chemical Society.
FAU - Mishra, Anjali
AU  - Mishra A
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
AD  - Academy of Scientific & Innovative Research (AcSIR), Anusandhan Bhawan, Rafi 
      Marg, New Delhi 110 001, India.
FAU - Chhonker, Yashpal Singh
AU  - Chhonker YS
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
FAU - Bisen, Amol Chhatrapati
AU  - Bisen AC
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
FAU - Prasad, Yarra Durga
AU  - Prasad YD
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
FAU - Tulsankar, Sachin Laxman
AU  - Tulsankar SL
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
AD  - Academy of Scientific & Innovative Research (AcSIR), Anusandhan Bhawan, Rafi 
      Marg, New Delhi 110 001, India.
FAU - Chandasana, Hardik
AU  - Chandasana H
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
AD  - Academy of Scientific & Innovative Research (AcSIR), Anusandhan Bhawan, Rafi 
      Marg, New Delhi 110 001, India.
FAU - Dey, Tushar
AU  - Dey T
AD  - Division of Animal Health, ICAR Research Complex for North Eastern Hill Region, 
      Meghalaya 793103, India.
FAU - Verma, Sarvesh Kumar
AU  - Verma SK
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
AD  - Jawaharlal Nehru University, New Delhi 110001, India.
FAU - Bala, Veenu
AU  - Bala V
AD  - Academy of Scientific & Innovative Research (AcSIR), Anusandhan Bhawan, Rafi 
      Marg, New Delhi 110 001, India.
AD  - Medicinal & Process Chemistry Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
FAU - Kanojiya, Sanjeev
AU  - Kanojiya S
AD  - Sophisticated Analytical Instruments Facility, CSIR-Central Drug Research 
      Institute, Lucknow 226031, India.
FAU - Ghatak, Sandeep
AU  - Ghatak S
AD  - Division of Animal Health, ICAR Research Complex for North Eastern Hill Region, 
      Meghalaya 793103, India.
FAU - Bhatta, Rabi Sankar
AU  - Bhatta RS
AD  - Pharmaceutics & Pharmacokinetics Division, CSIR-Central Drug Research Institute, 
      Lucknow 226031, India.
LA  - eng
PT  - Journal Article
DEP - 20201204
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC7745213
COIS- The authors declare no competing financial interest.
EDAT- 2020/12/22 06:00
MHDA- 2020/12/22 06:01
PMCR- 2020/12/04
CRDT- 2020/12/21 06:10
PHST- 2020/08/11 00:00 [received]
PHST- 2020/11/20 00:00 [accepted]
PHST- 2020/12/21 06:10 [entrez]
PHST- 2020/12/22 06:00 [pubmed]
PHST- 2020/12/22 06:01 [medline]
PHST- 2020/12/04 00:00 [pmc-release]
AID - 10.1021/acsomega.0c03863 [doi]
PST - epublish
SO  - ACS Omega. 2020 Dec 4;5(49):31584-31597. doi: 10.1021/acsomega.0c03863. 
      eCollection 2020 Dec 15.