PMID- 33346267
OWN - NLM
STAT- MEDLINE
DCOM- 20210118
LR  - 20210429
IS  - 1463-9084 (Electronic)
IS  - 1463-9076 (Linking)
VI  - 23
IP  - 1
DP  - 2021 Jan 6
TI  - Biamphiphilic ionic liquid based aqueous microemulsions as an efficient catalytic 
      medium for cytochrome c.
PG  - 320-328
LID - 10.1039/d0cp04513f [doi]
AB  - Considering the remarkable applicability of ionic liquids (ILs) in bio-catalysis 
      involving enzymes, herein, we report new IL based aqueous microemulsions as a 
      catalytic reactor for cytochrome c (Cyt-c). Microemulsions (muEs), comprising 
      water as the polar component, imidazolium (cation) and dioctylsulfosuccinate 
      (AOT) (anion) based biamphiphilic ionic liquid (BAIL) as the surfactant and a 
      hydrophobic ionic liquid (HIL) as the non-polar component have been prepared and 
      characterized. The use of BAIL has promoted the formation of muEs without any 
      co-surfactant, owing to its higher surface activity. The effect of ester- or 
      amide-functionalization of the alkyl chain of the imidazolium cation of BAILs on 
      the phase behavior of muEs has been investigated. The prepared muEs have been 
      characterized via conductivity, dynamic light scattering (DLS), UV-vis absorption 
      and steady-state fluorescence (using external polarity probes) techniques. The 
      prepared muEs have been employed as nano-reactors for exploring the catalytic 
      activity of Cyt-c. The formed BAIL-water nano-interfaces in reverse muEs have 
      exerted a positive effect on the catalytic activity of Cyt-c stored in a water 
      pool of reverse muEs. A five-fold higher rate constant in muEs as compared to 
      buffer establishes muEs as a better catalytic medium. Furthermore, the differing 
      nature of nano-interfaces created by BAILs and water in reverse muEs, depending on 
      the functionalization of the alkyl chain of the cationic part of BAIL, has 
      exerted varying influence on the catalytic activity of Cyt-c. It is expected that 
      the present work will result in providing a versatile platform for the creation 
      of new IL and water based muEs for bio-catalytic applications.
FAU - Kaur, Manvir
AU  - Kaur M
AD  - Department of Chemistry, UGC Sponsored Centre for Advanced Studies-II, Guru Nanak 
      Dev University, Amritsar-143005, India. tejwantsinghkang@gmail.com 
      tejwant.chem@gndu.ac.in.
FAU - Kaur, Harmandeep
AU  - Kaur H
FAU - Singh, Manpreet
AU  - Singh M
FAU - Singh, Gagandeep
AU  - Singh G
FAU - Kang, Tejwant Singh
AU  - Kang TS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Phys Chem Chem Phys
JT  - Physical chemistry chemical physics : PCCP
JID - 100888160
RN  - 0 (Emulsions)
RN  - 0 (Imidazoles)
RN  - 0 (Ionic Liquids)
RN  - 0 (Surface-Active Agents)
RN  - 059QF0KO0R (Water)
RN  - 10041-19-7 (Dioctyl Sulfosuccinic Acid)
RN  - 6JKA7MAH9C (Guaiacol)
RN  - 9007-43-6 (Cytochromes c)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Catalysis
MH  - Cytochromes c/*chemistry
MH  - Dioctyl Sulfosuccinic Acid/chemistry
MH  - Emulsions/*chemistry
MH  - Guaiacol/chemistry
MH  - Horses
MH  - Hydrogen Peroxide/chemistry
MH  - Imidazoles/chemistry
MH  - Ionic Liquids/*chemistry
MH  - Oxidation-Reduction
MH  - Surface-Active Agents/*chemistry
MH  - Water/chemistry
EDAT- 2020/12/22 06:00
MHDA- 2021/01/20 06:00
CRDT- 2020/12/21 08:45
PHST- 2020/12/22 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
PHST- 2020/12/21 08:45 [entrez]
AID - 10.1039/d0cp04513f [doi]
PST - ppublish
SO  - Phys Chem Chem Phys. 2021 Jan 6;23(1):320-328. doi: 10.1039/d0cp04513f.