PMID- 33348144
OWN - NLM
STAT- MEDLINE
DCOM- 20210121
LR  - 20210121
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 270
DP  - 2021 Feb 1
TI  - Exposure to O(3) during pregnancy and offspring asthma induced by OVA: Sensitive 
      window identification.
PG  - 116297
LID - S0269-7491(20)36986-4 [pii]
LID - 10.1016/j.envpol.2020.116297 [doi]
AB  - BACKGROUND: Evidence proved that gestational ozone (O(3)) exposure can increase 
      the risk of neonatal adverse respiratory outcomes, but offspring asthma is 
      unclear. OBJECTIVE: This study aimed to investigate whether gestational O(3) 
      exposure could exacerbate offspring asthma, and to research the differences in 
      effects of O(3) exposure at different gestational periods on offspring asthma. 
      METHODS: The pregnant ICR mice were randomly grouped and were administered O(3) 
      (air as control) at gestational day (GD) 1-6, 7-12, and 13-18, respectively. The 
      pups aged 2-4 weeks were treated with ovalbumin (OVA) to establish a model of 
      early life asthma. Asthma characteristics such as pulmonary inflammation, goblet 
      cell proliferation, airway remodeling, OVA-specific immunoglobulin (Ig) E 
      secretion and cytokines were examined. RESULTS: OVA sensitization and challenge 
      successfully induced asthma in offspring. Compared with the air control, 
      pulmonary inflammation infiltration, mucous secretion, the concentration of 
      OVA-specific IgE, the level of tumor necrosis factor (TNF)-alpha, and T helper (Th) 
      2-skewed response were significantly exacerbated when O(3) exposure at GD13-18 
      following inhaling OVA, while pulmonary inflammatory infiltration, mucus 
      secretion, and Th2-skewed response were not significantly changed when O(3) 
      exposure at both GD1-6 and GD7-12. What's more, the above indicators in asthmatic 
      offspring due to O(3) exposure at GD13-18 were more severe than at GD1-6 and 
      GD7-12. Interestingly, the signs of asthma only appeared in the offspring after 
      OVA inhalation. When offspring were not treated with OVA, the prenatal O(3) 
      exposure alone did not lead to asthmatic reactions in offspring. In addition, 
      O(3) exposure at GD13-18 markedly increased the number of neutrophils and 
      macrophages in Bronchoalveolar Lavage Fluid (BALF) of asthmatic offspring, and 
      significantly exacerbated Th2 immune imbalance in asthmatic offspring, but had no 
      effect on Th17 immune imbalance. CONCLUSION: Exposure to O(3) during pregnancy 
      aggravated asthma in offspring, in which the third trimester is the most 
      sensitive window.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Liu, Xinai
AU  - Liu X
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.
FAU - Fu, Lingling
AU  - Fu L
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.
FAU - Yang, Xiwei
AU  - Yang X
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.
FAU - Wang, Zhiping
AU  - Wang Z
AD  - Department of Occupational and Environmental Health, School of Public Health, 
      Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China. 
      Electronic address: zhipingw@sdu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20201213
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - *Asthma/chemically induced
MH  - Bronchoalveolar Lavage Fluid
MH  - Disease Models, Animal
MH  - Female
MH  - Lung
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred ICR
MH  - Ovalbumin
MH  - Pregnancy
OTO - NOTNLM
OT  - Asthma
OT  - Ozone
OT  - Prenatal exposure
OT  - Sensitive window
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/12/22 06:00
MHDA- 2021/01/22 06:00
CRDT- 2020/12/21 20:12
PHST- 2020/08/12 00:00 [received]
PHST- 2020/11/13 00:00 [revised]
PHST- 2020/12/10 00:00 [accepted]
PHST- 2020/12/22 06:00 [pubmed]
PHST- 2021/01/22 06:00 [medline]
PHST- 2020/12/21 20:12 [entrez]
AID - S0269-7491(20)36986-4 [pii]
AID - 10.1016/j.envpol.2020.116297 [doi]
PST - ppublish
SO  - Environ Pollut. 2021 Feb 1;270:116297. doi: 10.1016/j.envpol.2020.116297. Epub 
      2020 Dec 13.