PMID- 33352459
OWN - NLM
STAT- MEDLINE
DCOM- 20210616
LR  - 20210616
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 44
IP  - 1
DP  - 2021 Jan
TI  - Gene-PROBER - a tool to design polynucleotide probes for targeting microbial 
      genes.
PG  - 126173
LID - S0723-2020(20)30128-4 [pii]
LID - 10.1016/j.syapm.2020.126173 [doi]
AB  - Recent developments in fluorescence in situ hybridization (FISH) methods allow 
      the detection and visualization of the genes/genomic regions of bacteria, archaea 
      and infecting viruses at the single cell level. These methods use mixtures of 
      polynucleotides as probes to specifically detect the target of interest. 
      Gene-PROBER enables the design of polynucleotide mixtures for targeting genes or 
      genomic regions in microorganisms. It has four workflows, depending on the 
      availability of non-target sequences and the choice of probe synthesis, either by 
      chemical synthesis or by PCR. It outputs polynucleotides that are spread along 
      the target sequence and have similar melting properties. Therefore, such a 
      polynucleotide mixture can be used as a single probe, in a single hybridization 
      reaction. Gene-PROBER is a freely available web service that can be accessed at 
      http://gene-prober.icbm.de/, and is implemented in the R language using the Shiny 
      package.
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Moraru, Cristina
AU  - Moraru C
AD  - Department of the Biology of Geological Processes, Institute for Chemistry and 
      Biology of the Marine Environment, University of Oldenburg, 26111 Oldenburg, 
      Germany. Electronic address: liliana.cristina.moraru@uni-oldenburg.de.
LA  - eng
PT  - Journal Article
DEP - 20201207
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Polynucleotides)
SB  - IM
MH  - Archaea/genetics
MH  - Bacteria/genetics
MH  - Bacterial Typing Techniques
MH  - In Situ Hybridization, Fluorescence
MH  - Internet
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Probes/*chemistry
MH  - Polynucleotides/chemistry
MH  - *Software
OTO - NOTNLM
OT  - Direct-geneFISH
OT  - Gene detection
OT  - GeneFISH
OT  - Polynucleotide probe design
EDAT- 2020/12/23 06:00
MHDA- 2021/06/17 06:00
CRDT- 2020/12/22 20:11
PHST- 2020/10/02 00:00 [received]
PHST- 2020/11/06 00:00 [revised]
PHST- 2020/11/26 00:00 [accepted]
PHST- 2020/12/23 06:00 [pubmed]
PHST- 2021/06/17 06:00 [medline]
PHST- 2020/12/22 20:11 [entrez]
AID - S0723-2020(20)30128-4 [pii]
AID - 10.1016/j.syapm.2020.126173 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2021 Jan;44(1):126173. doi: 10.1016/j.syapm.2020.126173. 
      Epub 2020 Dec 7.