PMID- 33352641
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201231
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 9
IP  - 12
DP  - 2020 Dec 19
TI  - Clinical Biofilm Ring Test((R)) Reveals the Potential Role of beta-Lactams in the 
      Induction of Biofilm Formation by P. aeruginosa in Cystic Fibrosis Patients.
LID - 10.3390/pathogens9121065 [doi]
LID - 1065
AB  - Biofilms are characterized by high tolerance to antimicrobials. However, 
      conventional antibiograms are performed on planktonic microorganisms. Through the 
      clinical Biofilm Ring Test((R)) (cBRT), initially aimed to measure the adhesion 
      propensity of bacteria, we discerned a variable distribution of biofilm-producer 
      strains among P. aeruginosa samples isolated from expectorations of cystic 
      fibrosis (CF) patients. Despite a majority of spontaneous adherent isolates, few 
      strains remained planktonic after 5 h of incubation. Their analysis by an adapted 
      protocol of the cBRT revealed an induction of the biofilm early formation by 
      sub-inhibitory doses of beta-lactams. Microscopic observations of bacterial cultures 
      stained with Syto 9/Propidium Iodide (PI) confirmed the ability of antimicrobials 
      to increase either the bacterial biomass or the biovolume occupied by induced 
      sessile cells. Finally, the cBRT and its derivatives enabled to highlight in a 
      few hours the potential inducer property of antibiotics on bacterial adhesion. 
      This phenomenon should be considered carefully in the context of CF since 
      patients are constantly under fluctuating antimicrobial treatments. To conclude, 
      assays derived from the Biofilm Ring Test((R)) (BRT) device, not only define 
      efficient doses preventing biofilm formation, but could be useful for the 
      antimicrobial selection in CF, to avoid inducer molecules of the early biofilm 
      initiation.
FAU - Olivares, Elodie
AU  - Olivares E
AUID- ORCID: 0000-0001-9224-7665
AD  - Virulence Bacterienne Precoce UR7290, ITI Innovec, FMTS, Institut de 
      Bacteriologie, Universite de Strasbourg, 3 Rue Koeberle, 67000 Strasbourg, 
      France.
AD  - BioFilm Pharma SAS, 317 Avenue Jean Jaures, 69007 Lyon, France.
FAU - Tasse, Jason
AU  - Tasse J
AUID- ORCID: 0000-0002-7496-7594
AD  - BioFilm Pharma SAS, 317 Avenue Jean Jaures, 69007 Lyon, France.
FAU - Badel-Berchoux, Stephanie
AU  - Badel-Berchoux S
AUID- ORCID: 0000-0002-7212-8199
AD  - BioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, France.
FAU - Provot, Christian
AU  - Provot C
AD  - BioFilm Pharma SAS, 317 Avenue Jean Jaures, 69007 Lyon, France.
AD  - BioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, France.
FAU - Prevost, Gilles
AU  - Prevost G
AD  - Virulence Bacterienne Precoce UR7290, ITI Innovec, FMTS, Institut de 
      Bacteriologie, Universite de Strasbourg, 3 Rue Koeberle, 67000 Strasbourg, 
      France.
FAU - Bernardi, Thierry
AU  - Bernardi T
AD  - BioFilm Pharma SAS, 317 Avenue Jean Jaures, 69007 Lyon, France.
AD  - BioFilm Control SAS, Rue Emile Duclaux, 63360 Saint Beauzire, France.
LA  - eng
PT  - Journal Article
DEP - 20201219
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC7766936
OTO - NOTNLM
OT  - Pseudomonas aeruginosa
OT  - antibiotics
OT  - biofilm
OT  - clinical Biofilm Ring Test(R)
OT  - cystic fibrosis
OT  - early bacterial adhesion induction
COIS- Authors E.O., C.P., S.B.-B., and J.T. were employed by the companies BioFilm 
      Pharma SAS and BioFilm Control SAS. Author T.B. is the inventor of the BRT 
      device. The remaining authors declare that the research was conducted in the 
      absence of any commercial or financial relationships that could be construed as a 
      potential conflict of interest.
EDAT- 2020/12/24 06:00
MHDA- 2020/12/24 06:01
PMCR- 2020/12/19
CRDT- 2020/12/23 01:01
PHST- 2020/12/01 00:00 [received]
PHST- 2020/12/15 00:00 [revised]
PHST- 2020/12/16 00:00 [accepted]
PHST- 2020/12/23 01:01 [entrez]
PHST- 2020/12/24 06:00 [pubmed]
PHST- 2020/12/24 06:01 [medline]
PHST- 2020/12/19 00:00 [pmc-release]
AID - pathogens9121065 [pii]
AID - pathogens-09-01065 [pii]
AID - 10.3390/pathogens9121065 [doi]
PST - epublish
SO  - Pathogens. 2020 Dec 19;9(12):1065. doi: 10.3390/pathogens9121065.