PMID- 33355358
OWN - NLM
STAT- MEDLINE
DCOM- 20210610
LR  - 20211214
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 53
IP  - 2
DP  - 2021 Feb 4
TI  - Overexpression of LVRN impedes the invasion of trophoblasts by inhibiting 
      epithelial-mesenchymal transition.
PG  - 249-257
LID - 10.1093/abbs/gmaa167 [doi]
AB  - Laeverin (LVRN) was first detected on the outer layer of the chorion laeve and 
      migrating extravillous trophoblasts (EVTs). It is an enzyme that plays an 
      important role in the placentation and pathophysiology of preeclampsia (PE). 
      Previous studies have indicated that LVRN may be required for the invasion of 
      human trophoblast cells. Paradoxically, LVRN was found to be highly expressed in 
      the trophoblasts of PE patients with impaired invasive capacities. In this study, 
      we detected the expression of LVRN in the placentas of PE patients (n=5) and 
      normal term pregnancy women (n=5) as a control group by immunohistochemistry. 
      LVRN was elevated in decidua (P=0.0083) and villi (P=0.0079) of PE patients. 
      Next, LVRN was overexpressed via adeno-associated virus-mediated gene transfer in 
      trophoblastic cell lines HTR8, Swan71, and JAR. Matrigel transwell assay and 
      wound healing assay showed that overexpression of LVRN impeded the invasion of 
      these three cell lines. Western blot analysis showed that LVRN overexpression 
      caused downregulation of N-cadherin and vimentin and upregulation of E-cadherin, 
      suggesting the inhibitory role of LVRN in epithelial-mesenchymal transition 
      (EMT). Moreover, our data indicated that long noncoding RNA NONSTAT103348 
      (lnc10-7) was elevated in PE patients. Silencing lnc10-7 led to decreased LVRN 
      expression. Taken together, although the basal level of LVRN may be crucial for 
      cell invasion, overexpression of LVRN may abrogate the cell invasiveness, 
      suggesting a multifaceted role of LVRN in the pathogenesis of PE.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of the 
      Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences. All 
      rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
FAU - Feng, Xuan
AU  - Feng X
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Wei, Zhi
AU  - Wei Z
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Zhang, Sai
AU  - Zhang S
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Zhou, Jiayi
AU  - Zhou J
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Wu, Jing
AU  - Wu J
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Luan, Baoxin
AU  - Luan B
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Du, Yan
AU  - Du Y
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
FAU - Zhao, Hongbo
AU  - Zhao H
AD  - Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
AD  - Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, 
      Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Pregnancy Proteins)
RN  - EC 3.4.- (LVRN protein, human)
RN  - EC 3.4.- (Metalloproteases)
SB  - IM
MH  - *Epithelial-Mesenchymal Transition
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Metalloproteases/*biosynthesis/genetics
MH  - Pregnancy Proteins/*biosynthesis/genetics
MH  - Trophoblasts/*metabolism
OTO - NOTNLM
OT  - LVRN
OT  - epithelial-mesenchymal transition
OT  - extravillous trophoblast
OT  - invasion
OT  - lnc10-7
EDAT- 2020/12/24 06:00
MHDA- 2021/06/11 06:00
CRDT- 2020/12/23 08:42
PHST- 2020/06/04 00:00 [received]
PHST- 2020/12/24 06:00 [pubmed]
PHST- 2021/06/11 06:00 [medline]
PHST- 2020/12/23 08:42 [entrez]
AID - 6044939 [pii]
AID - 10.1093/abbs/gmaa167 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2021 Feb 4;53(2):249-257. doi: 
      10.1093/abbs/gmaa167.