PMID- 33356012
OWN - NLM
STAT- MEDLINE
DCOM- 20211111
LR  - 20211111
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 21
IP  - 3
DP  - 2021 Mar
TI  - Gradient Mineralized and Porous Double-Network Hydrogel Effectively Induce the 
      Differentiation of BMSCs into Osteochondral Tissue In Vitro for Potential 
      Application in Cartilage Repair.
PG  - e2000323
LID - 10.1002/mabi.202000323 [doi]
AB  - At present, it is a considerable challenge to mimic the complex architecture of 
      osteochondral (OC) tissue. In this study, a porous and gradient mineralized 
      double-network hydrogel is synthesized and used to induce bone marrow mesenchymal 
      stem cells (BMSCs) to differentiate into the desired OC tissue depending only on 
      the material and mechanical properties. Physical and chemical characterizations 
      show that hydroxyapatite nanoparticles grow and fill into the pores of the 
      hydrogel, and their content presents a gradient change in different layers of 
      hydrogel. The synthesized hydrogel has excellent mechanical properties and the 
      compression strength with different mineralization degrees varies from 27 to 380 
      kPa, which fully meets the needs of increased mechanical strength of articular 
      cartilage from the surface to the deep layer. Besides, the synthesized hydrogel 
      has good biocompatibility that can promote the proliferation and growth of BMSCs. 
      More importantly, the results of histochemistry, immunohistochemistry, and real 
      time polymerase chain reaction show that gradient mineralized hydrogel can induce 
      BMSCs to differentiate into the desired chondrocytes and osteoblasts in different 
      layers of hydrogels, indicating that OC tissues can be successfully constructed 
      through a simple induction differentiation of gradient mineralized hydrogel.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Fan, Zengjie
AU  - Fan Z
AUID- ORCID: 0000-0002-2865-8475
AD  - School of Stomatology, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China.
FAU - Chen, Zizi
AU  - Chen Z
AD  - School of Stomatology, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - School of Stomatology, Lanzhou University, Lanzhou, Gansu, 730000, P. R. China.
FAU - Nie, Yingying
AU  - Nie Y
AD  - Institute of Sensing Technology, Gansu Academy of Sciences, Lanzhou, Gansu, 
      730000, P. R. China.
FAU - Xu, Shumei
AU  - Xu S
AD  - Department of General Surgery, the 940th Hospital of Joint Logistics Support 
      Force, PLA, Lanzhou, Gansu, 730050, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201223
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type II)
RN  - 0 (Hydrogels)
RN  - 0 (Proteoglycans)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - Animals
MH  - Bone Regeneration/*drug effects
MH  - Cartilage, Articular/*pathology
MH  - Cell Differentiation/*drug effects
MH  - Chondrogenesis/drug effects
MH  - Coculture Techniques
MH  - Collagen Type I/metabolism
MH  - Collagen Type II/metabolism
MH  - Fibrinogen/metabolism
MH  - Hydrogels/*pharmacology
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - Porosity
MH  - Proteoglycans/metabolism
MH  - Rabbits
MH  - Spectroscopy, Fourier Transform Infrared
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - BMSCs
OT  - cartilage repair
OT  - double-network hydrogel
OT  - gradient mineralization
OT  - osteochondral tissue
EDAT- 2020/12/29 06:00
MHDA- 2021/11/12 06:00
CRDT- 2020/12/28 07:45
PHST- 2020/12/02 00:00 [revised]
PHST- 2020/09/15 00:00 [received]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2021/11/12 06:00 [medline]
PHST- 2020/12/28 07:45 [entrez]
AID - 10.1002/mabi.202000323 [doi]
PST - ppublish
SO  - Macromol Biosci. 2021 Mar;21(3):e2000323. doi: 10.1002/mabi.202000323. Epub 2020 
      Dec 23.