PMID- 33356413
OWN - NLM
STAT- MEDLINE
DCOM- 20210913
LR  - 20210913
IS  - 1945-8932 (Electronic)
IS  - 1945-8932 (Linking)
VI  - 35
IP  - 5
DP  - 2021 Sep
TI  - LPCAT2 Methylation, a Novel Biomarker for the Severity of Cedar Pollen Allergic 
      Rhinitis in Japan.
PG  - 631-639
LID - 10.1177/1945892420983646 [doi]
AB  - BACKGROUND: Recently, the role of the epigenome in allergies has been receiving 
      increasing attention. Although several genes that are methylated in relation to 
      serum immunoglobulin E (IgE) concentration have been reported by epigenome-wide 
      association studies, little is known about the DNA methylation sites associated 
      with the symptoms and severity of cedar pollinosis (CP). OBJECTIVE: Our aim was 
      to analyze the association between DNA methylation and the symptoms and severity 
      of CP in peripheral blood mononuclear cells (PBMCs) and nasal mucosa scraping 
      cells (NMSCs). METHODS: We recruited 70 participants during the cedar pollen 
      dispersal season. IgE levels were measured by a fluorescence enzyme immunoassay. 
      We analyzed DNA methylation of acyl-CoA thioesterase 7 (ACOT7), mucin 4 (MUC4), 
      schlafen 12 (SLFN12), lysophosphatidylcholine acyltransferase 2 (LPCAT2), and 
      interleukin-4 (IL4) in PBMCs and NMSCs using bisulfite next-generation 
      sequencing; the correlation of DNA methylation with non-specific IgE and cedar 
      pollen-specific IgE levels in peripheral blood samples was also investigated. 
      Symptom severity and DNA methylation were investigated in 15 untreated CP 
      patients. RESULTS: Non-specific IgE levels showed a significant negative 
      correlation with average IL4 methylation in PBMCs (r = -0.46, P < 0.0001) but not 
      with methylation of ACOT7, MUC4, SLFN12, and LPCAT2. Cedar pollen-specific IgE 
      levels showed a significant negative correlation with average IL4 and MUC4 
      methylation in PBMCs (r = -0.31, P = 0.01 and r = -0.241, P = 0.046, 
      respectively) but not with methylation of ACOT7, SLFN12, and LPCAT2. The 
      methylation of some genes in NMSCs was not significantly correlated with IgE 
      levels. The mean methylation of LPCAT2 in NMSCs showed a decreasing trend with 
      increasing severity of CP (P = 0.027). CONCLUSION: LPCAT2 methylation in NMSCs 
      may reflect the severity of CP and could be used as a novel biomarker to identify 
      suitable treatment options for CP.
FAU - Watanabe, Hiroyuki
AU  - Watanabe H
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, 
      Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Kofu, 
      Japan.
AD  - Department of Health Sciences, Graduate School of Interdisciplinary Research, 
      University of Yamanashi, Kofu, Japan.
FAU - Miyake, Kunio
AU  - Miyake K
AUID- ORCID: 0000-0001-9196-2229
AD  - Department of Health Sciences, Graduate School of Interdisciplinary Research, 
      University of Yamanashi, Kofu, Japan.
FAU - Matsuoka, Tomokazu
AU  - Matsuoka T
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, 
      Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Kofu, 
      Japan.
FAU - Kojima, Reiji
AU  - Kojima R
AUID- ORCID: 0000-0002-9712-889X
AD  - Department of Health Sciences, Graduate School of Interdisciplinary Research, 
      University of Yamanashi, Kofu, Japan.
FAU - Sakurai, Daiju
AU  - Sakurai D
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, 
      Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Kofu, 
      Japan.
FAU - Masuyama, Keisuke
AU  - Masuyama K
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, 
      Graduate Faculty of Interdisciplinary Research, University of Yamanashi, Kofu, 
      Japan.
AD  - Department of Otorhinolaryngology, Suwa Central Hospital, Chino, Japan.
FAU - Yamagata, Zentaro
AU  - Yamagata Z
AD  - Department of Health Sciences, Graduate School of Interdisciplinary Research, 
      University of Yamanashi, Kofu, Japan.
LA  - eng
PT  - Journal Article
DEP - 20201223
PL  - United States
TA  - Am J Rhinol Allergy
JT  - American journal of rhinology & allergy
JID - 101490775
RN  - 0 (Allergens)
RN  - 0 (Biomarkers)
RN  - EC 2.3.1.23 (1-Acylglycerophosphocholine O-Acyltransferase)
RN  - EC 2.3.1.23 (LPCAT2 protein, human)
SB  - IM
MH  - 1-Acylglycerophosphocholine O-Acyltransferase
MH  - Allergens
MH  - Biomarkers
MH  - DNA Methylation
MH  - Humans
MH  - Japan
MH  - *Leukocytes, Mononuclear
MH  - Pollen
MH  - *Rhinitis, Allergic
OTO - NOTNLM
OT  - DNA methylation
OT  - Epigenetics
OT  - IL4
OT  - IgE antibody
OT  - LPCAT2
OT  - allergic rhinitis
OT  - bisulfite sequencing
OT  - cedar pollen
OT  - nasal mucosa
OT  - severity
EDAT- 2020/12/29 06:00
MHDA- 2021/09/14 06:00
CRDT- 2020/12/28 10:07
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2021/09/14 06:00 [medline]
PHST- 2020/12/28 10:07 [entrez]
AID - 10.1177/1945892420983646 [doi]
PST - ppublish
SO  - Am J Rhinol Allergy. 2021 Sep;35(5):631-639. doi: 10.1177/1945892420983646. Epub 
      2020 Dec 23.