PMID- 33357907
OWN - NLM
STAT- MEDLINE
DCOM- 20210409
LR  - 20210409
IS  - 1879-1344 (Electronic)
IS  - 0144-8617 (Linking)
VI  - 254
DP  - 2021 Feb 15
TI  - In vitro biological response of human osteoblasts in 3D chitosan sponges with 
      controlled degree of deacetylation and molecular weight.
PG  - 117434
LID - S0144-8617(20)31607-6 [pii]
LID - 10.1016/j.carbpol.2020.117434 [doi]
AB  - We have studied the effect of chitosan sponges, produced from chitosan batches 
      with distinct degree of deacetylation (DDA) and molecular weight (Mw), on the 
      adhesion, growth and differentiation of primary human osteoblasts with an aim to 
      offer a suitable tool for guided bone regeneration. All the chitosan sponges 
      revealed similar microstructure, irrespective of the DDA (58, 73, 82, 88, and 91 
      %) and Mw (749, 547, 263, 215, and 170 kDa, respectively). Cell spreading was 
      higher on sponges having a higher DDA. Higher DDA induced a more pronounced 
      increase in alkaline phosphatase activity, osteopontin (OPN), vascular 
      endothelial growth factor-A (VEGF), interleukin-6 (IL-6), and reduction in 
      monocyte chemoattractant protein-1 (MCP-1), sclerostin (SOST) and dickkopf 
      related protein-1 as compared to lower DDA. Lower DDA induced the increased 
      secretion of osteoprotegerin and SOST as compared to higher DDA. The combination 
      of higher DDA and Mw induced an increased secretion of VEGF and IL-6, however 
      reduced the secretion of OPN as compared to chitosan with similar DDA but with 
      lower Mw. In summary, the variations in cellular responses to the different 
      chitosan sponges indicate a potential for individual tailoring of desired 
      responses in guided bone regeneration.
CI  - Copyright (c) 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Sukul, Mousumi
AU  - Sukul M
AD  - Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 
      0317 Oslo, Norway. Electronic address: mousumi.sukul@odont.uio.no.
FAU - Sahariah, Priyanka
AU  - Sahariah P
AD  - Faculty of Pharmaceutical Sciences, School of Health Sciences, University of 
      Iceland, Hofsvallagata 53, IS-107 Reykjavik, Iceland.
FAU - Lauzon, Helene L
AU  - Lauzon HL
AD  - Primex ehf, Oskarsgata 7, 580-Siglufjordur, Iceland.
FAU - Borges, Joao
AU  - Borges J
AD  - Department of Chemistry, CICECO - Aveiro Institute of Materials, University of 
      Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.
FAU - Masson, Mar
AU  - Masson M
AD  - Faculty of Pharmaceutical Sciences, School of Health Sciences, University of 
      Iceland, Hofsvallagata 53, IS-107 Reykjavik, Iceland.
FAU - Mano, Joao F
AU  - Mano JF
AD  - Department of Chemistry, CICECO - Aveiro Institute of Materials, University of 
      Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal.
FAU - Haugen, Havard J
AU  - Haugen HJ
AD  - Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 
      0317 Oslo, Norway.
FAU - Reseland, Janne E
AU  - Reseland JE
AD  - Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, 
      0317 Oslo, Norway.
LA  - eng
PT  - Journal Article
DEP - 20201122
PL  - England
TA  - Carbohydr Polym
JT  - Carbohydrate polymers
JID - 8307156
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DKK1 protein, human)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (IL6 protein, human)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Osteoprotegerin)
RN  - 0 (SOST protein, human)
RN  - 0 (SPP1 protein, human)
RN  - 0 (TNFRSF11B protein, human)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 106441-73-0 (Osteopontin)
RN  - 9012-76-4 (Chitosan)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Acetylation
MH  - Adaptor Proteins, Signal Transducing/genetics/metabolism
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Regeneration
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chitosan/chemistry/*pharmacology
MH  - Delayed-Action Preparations/chemistry/*pharmacology
MH  - Gene Expression/*drug effects
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Interleukin-6/genetics/metabolism
MH  - Models, Biological
MH  - Molecular Structure
MH  - Molecular Weight
MH  - Osteoblasts/cytology/*drug effects/metabolism
MH  - Osteopontin/genetics/metabolism
MH  - Osteoprotegerin/genetics/metabolism
MH  - Primary Cell Culture
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - Chitosan
OT  - Degree of deacetylation
OT  - Molecular weight
OT  - Osteoblasts
EDAT- 2020/12/29 06:00
MHDA- 2021/04/10 06:00
CRDT- 2020/12/28 10:26
PHST- 2020/05/20 00:00 [received]
PHST- 2020/10/03 00:00 [revised]
PHST- 2020/11/19 00:00 [accepted]
PHST- 2020/12/28 10:26 [entrez]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
AID - S0144-8617(20)31607-6 [pii]
AID - 10.1016/j.carbpol.2020.117434 [doi]
PST - ppublish
SO  - Carbohydr Polym. 2021 Feb 15;254:117434. doi: 10.1016/j.carbpol.2020.117434. Epub 
      2020 Nov 22.