PMID- 33361540
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210105
IS  - 1226-4512 (Print)
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Linking)
VI  - 25
IP  - 1
DP  - 2021 Jan 1
TI  - Immunohistochemical detection of GluA1 subunit of AMPA receptor in the rat 
      nucleus accumbens following cocaine exposure.
PG  - 79-85
LID - 10.4196/kjpp.2021.25.1.79 [doi]
AB  - alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors are 
      differentially regulated in the nucleus accumbens (NAcc) of the brain after 
      cocaine exposure. However, these results are supported only by biochemical and 
      electrophysiological methods, but have not been validated with 
      immunohistochemistry. To overcome the restriction of antigen loss on the 
      postsynaptic target molecules that occurs during perfusion-fixation, we adopted 
      an immersion-fixation method that enabled us to immunohistochemically quantify 
      the expression levels of the AMPA receptor GluA1 subunit in the NAcc. 
      Interestingly, compared to saline exposure, cocaine significantly increased the 
      immunofluorescence intensity of GluA1 in two sub-regions, the core and the shell, 
      of the NAcc on withdrawal day 21 following cocaine exposure, which led to 
      locomotor sensitization. Increases in GluA1 intensity were observed in both the 
      extra-post synaptic density (PSD) and PSD areas in the two sub-regions of the 
      NAcc. These results clearly indicate that AMPA receptor plasticity, as 
      exemplified by GluA1, in the NAcc can be visually detected by 
      immunohistochemistry and confocal imaging. These results expand our understanding 
      of the molecular changes occurring in neuronal synapses by adding a new form of 
      analysis to conventional biochemical and electrophysiological methods.
FAU - Cai, Wen Ting
AU  - Cai WT
AD  - Department of Physiology, Graduate School of Medical Sciences, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Han, Joonyeup
AU  - Han J
AD  - Department of Physiology, Graduate School of Medical Sciences, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Kim, Wha Young
AU  - Kim WY
AD  - Department of Physiology, Graduate School of Medical Sciences, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Kim, Jeong-Hoon
AU  - Kim JH
AD  - Department of Physiology, Graduate School of Medical Sciences, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC7756536
OTO - NOTNLM
OT  - Cocaine
OT  - Glutamate receptor
OT  - Nucleus accumbens
OT  - Tissue fixation
COIS- CONFLICTS OF INTEREST The authors declare no conflicts of interest.
EDAT- 2020/12/29 06:00
MHDA- 2020/12/29 06:01
PMCR- 2021/01/01
CRDT- 2020/12/28 11:42
PHST- 2020/11/06 00:00 [received]
PHST- 2020/11/20 00:00 [revised]
PHST- 2020/11/23 00:00 [accepted]
PHST- 2020/12/28 11:42 [entrez]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2020/12/29 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - kjpp.2021.25.1.79 [pii]
AID - kjpp-25-1-79 [pii]
AID - 10.4196/kjpp.2021.25.1.79 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2021 Jan 1;25(1):79-85. doi: 
      10.4196/kjpp.2021.25.1.79.