PMID- 33363606
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231110
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Jan
TI  - Dendritic cell-CD4(+) T cell interaction: The differential role of IL-4/IL-13 in 
      serum IgE levels in house dust mite allergic patients.
PG  - 95
LID - 10.3892/etm.2020.9527 [doi]
LID - 95
AB  - Allergic asthma is a chronic airway inflammatory disorder triggered by inhalant 
      allergens. Interleukin (IL)-4 and IL-13 play a main role in the generation of T 
      helper cell type 2 (Th2) immune response, induction of immunoglobulin E (IgE) 
      synthesis and persistence of airway inflammation. The aim of the present study 
      was to investigate the influence of Dermatophagoides pteronyssinus allergen Der p 
      1, the major allergen of house dust mite, on the synthesis of IL-4 and IL-13 by 
      monocyte-derived dendritic cells (DCs) and naive CD4(+) T cells cocultured with 
      DCs, as well as their role in the production of serum IgE, in house dust mite 
      (HDM) allergic patients. Peripheral blood mononuclear cells (PBMCs) were isolated 
      from venous blood of patients allergic to HDM and healthy donors and incubated 
      with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 to 
      generate immature DCs. The obtained cells were stimulated for 24 h with Der p 1 
      to induce DC maturation, washed, and afterwards cocultured for 24 h with 
      autologous naive CD4(+) T cells. Culture supernatants were harvested for IL-4, 
      IL-13 and IFN-gamma level measurements. DCs stimulation with Der p 1 induced higher 
      synthesis of IL-4 and IL-13 in HDM allergic patients, compared to healthy donors. 
      The allergic group showed significant correlation between IL-13 production by Der 
      p 1-pulsed DCs, and total serum IgE and IL-4 production of the same cells and Der 
      p-specific IgE. To conclude, IL-4 and IL-13 are critically related to the 
      regulation of serum IgE production in patients with allergic asthma. The 
      relevance of these two cytokines in the pathophysiology of Th2 asthma endotype 
      makes them an appropriate target in its management.
CI  - Copyright: (c) Cernescu et al.
FAU - Cernescu, Luminita Daniela
AU  - Cernescu LD
AD  - Discipline of Dermatovenerology, Department XIV Microbiology, 'Victor Babes' 
      University of Medicine and Pharmacy Timisoara, Timisoara RO-300041, Romania.
FAU - Haidar, Laura
AU  - Haidar L
AD  - Discipline of Physiology, Department III Functional Sciences, 'Victor Babes' 
      University of Medicine and Pharmacy Timisoara, Timisoara RO-300041, Romania.
AD  - County Emergency Clinical Hospital 'Pius Brinzeu' Timisoara-Centre for Gene and 
      Cellular Therapies in The Treatment of Cancer OncoGen, Timisoara RO-300723, 
      Romania.
FAU - Panaitescu, Carmen
AU  - Panaitescu C
AD  - Discipline of Physiology, Department III Functional Sciences, 'Victor Babes' 
      University of Medicine and Pharmacy Timisoara, Timisoara RO-300041, Romania.
AD  - County Emergency Clinical Hospital 'Pius Brinzeu' Timisoara-Centre for Gene and 
      Cellular Therapies in The Treatment of Cancer OncoGen, Timisoara RO-300723, 
      Romania.
LA  - eng
PT  - Journal Article
DEP - 20201126
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7725010
OTO - NOTNLM
OT  - CD4+ T cells
OT  - Ig E
OT  - allergic asthma
OT  - dendritic cells
OT  - interleukin-13
OT  - interleukin-4
EDAT- 2020/12/29 06:00
MHDA- 2020/12/29 06:01
PMCR- 2020/11/26
CRDT- 2020/12/28 12:00
PHST- 2020/09/11 00:00 [received]
PHST- 2020/10/12 00:00 [accepted]
PHST- 2020/12/28 12:00 [entrez]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2020/12/29 06:01 [medline]
PHST- 2020/11/26 00:00 [pmc-release]
AID - ETM-0-0-09527 [pii]
AID - 10.3892/etm.2020.9527 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Jan;21(1):95. doi: 10.3892/etm.2020.9527. Epub 2020 Nov 26.