PMID- 33364623
OWN - NLM
STAT- MEDLINE
DCOM- 20220317
LR  - 20220317
IS  - 2666-1667 (Electronic)
IS  - 2666-1667 (Linking)
VI  - 2
IP  - 1
DP  - 2021 Mar 19
TI  - Profiling of mouse macrophage lipidome using direct infusion shotgun mass 
      spectrometry.
PG  - 100235
LID - 10.1016/j.xpro.2020.100235 [doi]
LID - 100235
AB  - Immune cells, such as macrophages, reprogram their lipid metabolism in response 
      to the activation of pattern recognition receptors (e.g., TLRs, NLRs) and 
      cytokine receptors (e.g., interferons, interleukins). Profiling these changes can 
      be achieved with shotgun mass spectrometry. This protocol provides step-by-step 
      instructions on the generation and stimulation of bone marrow-derived macrophages 
      (BMDMs), sample collection, and lipid extraction for profiling the macrophage 
      lipidome. For complete details on the use and execution of this protocol, please 
      refer to Hsieh et al. (2020).
CI  - (c) 2020 The Authors.
FAU - Hsieh, Wei-Yuan
AU  - Hsieh WY
AD  - Department of Microbiology, Immunology and Molecular Genetics, University of 
      California, Los Angeles, Los Angeles, CA 90095, USA.
FAU - Williams, Kevin J
AU  - Williams KJ
AD  - Department of Biological Chemistry, University of California, Los Angeles, Los 
      Angeles, CA 90095, USA.
AD  - UCLA Lipidomics Lab, University of California, Los Angeles, Los Angeles, CA 
      90095, USA.
FAU - Su, Baolong
AU  - Su B
AD  - Department of Biological Chemistry, University of California, Los Angeles, Los 
      Angeles, CA 90095, USA.
AD  - UCLA Lipidomics Lab, University of California, Los Angeles, Los Angeles, CA 
      90095, USA.
FAU - Bensinger, Steven J
AU  - Bensinger SJ
AD  - Department of Microbiology, Immunology and Molecular Genetics, University of 
      California, Los Angeles, Los Angeles, CA 90095, USA.
AD  - UCLA Lipidomics Lab, University of California, Los Angeles, Los Angeles, CA 
      90095, USA.
LA  - eng
GR  - P01 HL146358/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20201219
PL  - United States
TA  - STAR Protoc
JT  - STAR protocols
JID - 101769501
MH  - Animals
MH  - *Lipid Metabolism
MH  - *Lipidomics
MH  - Macrophages/*metabolism
MH  - *Mass Spectrometry
MH  - Mice
PMC - PMC7753944
OTO - NOTNLM
OT  - Cell culture
OT  - High Throughput Screening
OT  - Immunology
OT  - Mass Spectrometry
OT  - Metabolism
OT  - Model Organisms
COIS- The authors declare no competing interests.
EDAT- 2020/12/29 06:00
MHDA- 2020/12/29 06:01
PMCR- 2020/12/19
CRDT- 2020/12/28 12:03
PHST- 2020/12/28 12:03 [entrez]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2020/12/29 06:01 [medline]
PHST- 2020/12/19 00:00 [pmc-release]
AID - S2666-1667(20)30222-7 [pii]
AID - 100235 [pii]
AID - 10.1016/j.xpro.2020.100235 [doi]
PST - epublish
SO  - STAR Protoc. 2020 Dec 19;2(1):100235. doi: 10.1016/j.xpro.2020.100235. 
      eCollection 2021 Mar 19.