PMID- 33365342
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 2322-1984 (Print)
IS  - 2322-2271 (Electronic)
IS  - 2322-1984 (Linking)
VI  - 14
IP  - 2
DP  - 2020 Jun
TI  - Purification and Partial Characterization of Agglutinin Lectin from Heamolymph of 
      German Cockroach, Blattella germanica.
PG  - 144-152
LID - 10.18502/jad.v14i2.3732 [doi]
AB  - BACKGROUND: Lectin molecules have crucial biological role in insects' immune 
      system. The aim of present study was to find the agglutinin activities in 
      haemolymph of German cockroach, Belatella germanica with appropriate screening 
      and purification. METHODS: The heamolymph of cockroach was collected and 
      agglutinin test performed against different animal and human red blood cells 
      (RBC). Then sugar inhibition assay was carried out to find carbohydrate specific 
      binding lectin. The proteins of haemolymph was purified using ion-exchange 
      chromatography (HPLC) and each fraction was tested for agglutinin activity. 
      Finally the molecular weight of the agglutinin protein was determined using 
      SDS-page. RESULTS: The most agglutinin activity of haemolymph was found against 
      RBC of mouse at titer 1/128ml/L dilution and sugar inhibition assay showed that 
      fucos, N-acetyglucoseamine and galactose reduced titer of agglutinin to (1/2)ml/L. 
      Only one fraction of heamolymph at rotation time of 36 minute showed agglutinin 
      activity. The molecular weight of this lectin was measured as 120Kds. CONCLUSION: 
      The range of agglutinin activities against different RBC indicates that the 
      isolated lectin is not specific for a particular carbohydrate. In addition, the 
      isolated lectin at low concentration present in heamolymph should be an innate 
      lactin not secreted, because we found it without any trigger immunity of the 
      insect.
CI  - Copyright(c) Iranian Society of Medical Entomology & Tehran University of Medical 
      Sciences.
FAU - Nabavi, Zohreh
AU  - Nabavi Z
AD  - Department of Medical Entomology and Vector Control, School of Public Health, 
      Tehran University of Medical Sciences, Tehran, Iran.
FAU - Baniardalani, Mozhgan
AU  - Baniardalani M
AD  - Department of Medical Entomology and Vector Control, School of Public Health, 
      Tehran University of Medical Sciences, Tehran, Iran.
FAU - Basseri, Hamidreza
AU  - Basseri H
AD  - Department of Medical Entomology and Vector Control, School of Public Health, 
      Tehran University of Medical Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200630
PL  - Iran
TA  - J Arthropod Borne Dis
JT  - Journal of arthropod-borne diseases
JID - 101593448
PMC - PMC7738926
OTO - NOTNLM
OT  - German cockroach
OT  - Heamolymph
OT  - Lectin
EDAT- 2020/12/29 06:00
MHDA- 2020/12/29 06:01
PMCR- 2020/06/30
CRDT- 2020/12/28 12:07
PHST- 2018/02/23 00:00 [received]
PHST- 2020/05/28 00:00 [accepted]
PHST- 2020/12/28 12:07 [entrez]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2020/12/29 06:01 [medline]
PHST- 2020/06/30 00:00 [pmc-release]
AID - jad-14-144 [pii]
AID - 10.18502/jad.v14i2.3732 [doi]
PST - epublish
SO  - J Arthropod Borne Dis. 2020 Jun 30;14(2):144-152. doi: 10.18502/jad.v14i2.3732. 
      eCollection 2020 Jun.