PMID- 33369403
OWN - NLM
STAT- MEDLINE
DCOM- 20210218
LR  - 20210218
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 93
IP  - 3
DP  - 2021 Jan 26
TI  - Click Chemistry-Actuated Digital DNA Walker Confined on a Single Particle toward 
      Absolute MicroRNA Quantification.
PG  - 1620-1626
LID - 10.1021/acs.analchem.0c04073 [doi]
AB  - A versatile single magnetic nanoparticle (MNP)-confined, click chemistry-actuated 
      digital DNA walker (ddWalker) machine is devised for the absolute quantification 
      of microRNA (miRNA). This delicate ddWalker allows one target molecule to fix one 
      DNA walking leg on a single MNP, following the Poisson statistics through a 
      specific click chemical DNA ligation, which will initiate single molecule DNA 
      walking to stepwise cleave the molecular beacon tracks strictly constrained on 
      the leg-hold MNP without cross-particle reaction, fluorescently "lighting up" the 
      exact MNP. Accordingly, the initial miRNA input can be digitally and faithfully 
      reflected by the number of fluorescent-positive MNPs counted by a total internal 
      reflection fluorescent microscope, enabling the absolute and precise miRNA 
      quantification down to the femtomolar level without external calibration. This 
      flexible ddWalker design provides a new digital signaling concept and elegantly 
      expands the toolbox for digital biosensing.
FAU - Qi, Yan
AU  - Qi Y
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Key Laboratory of 
      Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & 
      Chemical Engineering, Ministry of Education, Shaanxi Normal University, Xi'an 
      710119, Shaanxi Province, P. R. China.
FAU - Zhai, Yuqing
AU  - Zhai Y
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Key Laboratory of 
      Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & 
      Chemical Engineering, Ministry of Education, Shaanxi Normal University, Xi'an 
      710119, Shaanxi Province, P. R. China.
FAU - Fan, Wenjiao
AU  - Fan W
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Key Laboratory of 
      Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & 
      Chemical Engineering, Ministry of Education, Shaanxi Normal University, Xi'an 
      710119, Shaanxi Province, P. R. China.
FAU - Ren, Wei
AU  - Ren W
AUID- ORCID: 0000-0002-6537-6039
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Key Laboratory of 
      Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & 
      Chemical Engineering, Ministry of Education, Shaanxi Normal University, Xi'an 
      710119, Shaanxi Province, P. R. China.
FAU - Li, Zhengping
AU  - Li Z
AUID- ORCID: 0000-0002-7573-8822
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Key Laboratory of 
      Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & 
      Chemical Engineering, Ministry of Education, Shaanxi Normal University, Xi'an 
      710119, Shaanxi Province, P. R. China.
FAU - Liu, Chenghui
AU  - Liu C
AUID- ORCID: 0000-0001-8375-0242
AD  - Key Laboratory of Applied Surface and Colloid Chemistry, Key Laboratory of 
      Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & 
      Chemical Engineering, Ministry of Education, Shaanxi Normal University, Xi'an 
      710119, Shaanxi Province, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201228
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (MicroRNAs)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Biosensing Techniques
MH  - Click Chemistry
MH  - DNA/*chemistry
MH  - HCT116 Cells
MH  - Humans
MH  - Magnetite Nanoparticles/*chemistry
MH  - MicroRNAs/*analysis
MH  - Particle Size
MH  - Surface Properties
EDAT- 2020/12/29 06:00
MHDA- 2021/02/20 06:00
CRDT- 2020/12/28 13:14
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/12/28 13:14 [entrez]
AID - 10.1021/acs.analchem.0c04073 [doi]
PST - ppublish
SO  - Anal Chem. 2021 Jan 26;93(3):1620-1626. doi: 10.1021/acs.analchem.0c04073. Epub 
      2020 Dec 28.