PMID- 33369466
OWN - NLM
STAT- MEDLINE
DCOM- 20210901
LR  - 20231111
IS  - 2476-762X (Electronic)
IS  - 1513-7368 (Print)
IS  - 1513-7368 (Linking)
VI  - 21
IP  - 12
DP  - 2020 Dec 1
TI  - Cytotoxic and Apoptotic Effects of Luffa Cylindrica Leaves Extract against Acute 
      Lymphoblastic Leukemic Stem Cells.
PG  - 3661-3668
LID - 89396 [pii]
LID - 10.31557/APJCP.2020.21.12.3661 [doi]
AB  - BACKGROUND: Acute lymphoblastic leukemia (ALL) is an aggressive malignancy 
      defined by accumulation of lymphoblasts in the bone marrow. Leukemic stem cells 
      (LSCs) are the major cause of the recurrence and metastasis of ALL. This study 
      aimed to develop an effective anti-cancer agent targeting these LSCs. Luffa 
      Cylindrica (L.C.) leaves extract was selected to evaluate its effect on ALL via 
      eradicating the LSCs as it contains many active anti-cancer flavonoids. METHODS: 
      Thirty-two bone marrow samples of ALL patients were used in this study. LSCs 
      population was identified in the selected samples. Cell viability was measured by 
      MTT assay and flow cytometry. Cell cycle, apoptosis, proliferation marker; ki-67 
      and colony forming assay were further analyzed. RESULTS: This study revealed the 
      expression of CD34+/CD38+ cells in addition to CD34+/CD38- population and the 
      extract was effective against the two LSCs populations. MTT assay showed that 
      treated leukemic cells exhibited significant reduction in the viable cells in a 
      dose dependent manner with IC50 of 3 microg/microl which was then confirmed by flow 
      cytometry. Cell cycle analysis results showed significant reduction in the 
      percentage of cells treated with L.C. extract in both the S and G0/G1 phases, 
      with concomitant increase in the G2/M phase. Also, L.C. extract could effectively 
      induce apoptosis, inhibit proliferation and suppress colonogenecity of leukemic 
      cells. CONCLUSION: This study validated the medicinal potential of L.C. leaves 
      extract as a promising anti-leukemic agent targeting both LSCs and blasts in ALL 
      patients, which may be explained by the synergy found between its potent 
      flavonoids especially apigenin, luteolin and kaempferol.
FAU - Yehia, Shimaa
AU  - Yehia S
AD  - Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
FAU - Abdel-Salam, Ibrahim M
AU  - Abdel-Salam IM
AD  - Cancer Biology Department, National Cancer Institute, Cairo University, Cairo, 
      Egypt.
FAU - Elgamal, Basma M
AU  - Elgamal BM
AD  - Clinical Pathology Department, National Cancer Institute, Cairo University, 
      Cairo, Egypt.
FAU - El-Agamy, Basma
AU  - El-Agamy B
AD  - Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
FAU - Hamdy, Germine M
AU  - Hamdy GM
AD  - Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
FAU - Aldouski, Hala M
AU  - Aldouski HM
AD  - Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20201201
PL  - Thailand
TA  - Asian Pac J Cancer Prev
JT  - Asian Pacific journal of cancer prevention : APJCP
JID - 101130625
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antineoplastic Agents/chemistry/*pharmacology
MH  - *Apoptosis
MH  - Cell Survival
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Infant
MH  - Leukemia, Myeloid, Acute/blood/*drug therapy/pathology
MH  - Luffa/*chemistry
MH  - Male
MH  - Middle Aged
MH  - Neoplastic Stem Cells/*drug effects/metabolism/pathology
MH  - Plant Extracts/*pharmacology
MH  - Plant Leaves/*chemistry
MH  - Young Adult
PMC - PMC8046306
OTO - NOTNLM
OT  - Acute Lymphoblastic Leukemia
OT  - CD34/CD38
OT  - Luffa cylindrica
OT  - leukemic stem cells
EDAT- 2020/12/29 06:00
MHDA- 2021/09/02 06:00
PMCR- 2021/02/01
CRDT- 2020/12/28 13:14
PHST- 2020/10/06 00:00 [received]
PHST- 2020/12/28 13:14 [entrez]
PHST- 2020/12/29 06:00 [pubmed]
PHST- 2021/09/02 06:00 [medline]
PHST- 2021/02/01 00:00 [pmc-release]
AID - 89396 [pii]
AID - 10.31557/APJCP.2020.21.12.3661 [doi]
PST - epublish
SO  - Asian Pac J Cancer Prev. 2020 Dec 1;21(12):3661-3668. doi: 
      10.31557/APJCP.2020.21.12.3661.