PMID- 33371795
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20231110
IS  - 1533-0338 (Electronic)
IS  - 1533-0346 (Print)
IS  - 1533-0338 (Linking)
VI  - 19
DP  - 2020 Jan-Dec
TI  - LncRNA-Associated ceRNA Network Reveals Novel Potential Biomarkers of Laryngeal 
      Squamous Cell Carcinoma.
PG  - 1533033820985787
LID - 10.1177/1533033820985787 [doi]
LID - 1533033820985787
AB  - OBJECTIVE: This study aims to construct a systematic mRNA-miRNA-lncRNA network to 
      identify novel lncRNAs and miRNAs biomarkers for laryngeal squamous cell 
      carcinoma (LSCC). METHODS: The mRNA, miRNA and lncRNA expression profiles of LSCC 
      were obtained from Gene Expression Omnibus (GEO) database. The differentially 
      expressed mRNAs, miRNAs and lncRNAs (DEmRNAs, DEmiRNAs and DElncRNAs) were 
      screened between LSCC tissues and controls. Functional analysis of DEmRNAs, 
      DEmRNAs targeted by DEmiRNAs and DEmRNAs targeted by DElncRNAs were respectively 
      performed. The miRWalk, starbase and DIANA-LncBase were respectively used to 
      predict DEmiRNAs-DEmRNAs, DElncRNAs-DEmRNAs and DElncRNAs-DEmiRNAs pairs. ceRNA 
      network was built by DEmiRNAs-DEmRNAs and DElncRNAs-DEmiRNAs pairs. LncRNA 
      subcellular localization was predicted using lncLocator. Using published The 
      Cancer Genome Atlas (TCGA) and external datasets (GSE127165 and GSE133632), we 
      also validated the expression of key DElncRNAs and DEmiRNAs in ceRNA network. The 
      diagnostic and prognostic value of candidate genes was evaluated by ROC curve 
      analysis and survival analysis, respectively. RESULTS: There were 5 mRNA 
      datasets, 3 miRNA datasets and 2 lncRNA datasets in this study. Totally, 2957 
      DEmRNAs, 61 DElncRNAs and 23 DEmiRNAs were identified. Functional analysis of 
      DEmRNAs shows that they were significantly enriched in cancer-related pathways, 
      such as DNA replication and extracellular matrix organization. There were 11 
      DEmiRNAs, 17 DElncRNAs and 967 DEmRNAs in the ceRNA network. Notably, 
      up-regulated lncRNA DGCR5-down-regulated has-miR-338-3p/has-miR-139-5p pairs in 
      this network were experimentally validated. Moreover, down-regulated AL121839.2, 
      down-regulated LINC02147, up-regulated AC079328.2, up-regulated AC004943.2 and 
      up-regulated HMGA2-AS1 were located in the cytoplasm. AL121839.2 and LINC02147 
      interacted with has-miR-1246. AC004943.2, AC079328.2 and HMGA2-AS1 targeted 
      has-miR-3185, has-miR-3137 and has-miR-582-5p, respectively. Based on the TCGA 
      and external datasets (GSE127165 and GSE133632), DGCR5 and AC004943.2 were 
      significantly up-regulated while AL121839.2 and LINC02147, has-miR-338-3p, 
      has-miR-139-5p and has-miR-582-5p were significantly down-regulated, which were 
      consistent with our integration analysis. DGCR5, AL121839.2, LINC02147, 
      AC004943.2, has-miR-338-3p, has-miR-139-5p and has-miR-582-5p could predict the 
      occurrence of LSCC. Survival analysis suggested that only, AL121839.2 has 
      potential prognostic value for LSCC. CONCLUSION: This study provided novel 
      insights into the ceRNA network and uncovered novel lncRNAs and miRNAs with 
      diagnostic value in LSCC.
FAU - Jing, Zhibin
AU  - Jing Z
AD  - Department of Otorhinolaryngology, Tianjin Medical University General Hospital, 
      Tianjin, China.
FAU - Guo, Sitong
AU  - Guo S
AD  - Department of Otorhinolaryngology, Tianjin Medical University General Hospital, 
      Tianjin, China.
FAU - Zhang, Peng
AU  - Zhang P
AD  - Department of Otorhinolaryngology, Tianjin Medical University General Hospital, 
      Tianjin, China.
FAU - Liang, Zheng
AU  - Liang Z
AUID- ORCID: 0000-0001-7033-5533
AD  - Department of Otorhinolaryngology, Tianjin Medical University General Hospital, 
      Tianjin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Technol Cancer Res Treat
JT  - Technology in cancer research & treatment
JID - 101140941
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - *Biomarkers, Tumor
MH  - Computational Biology
MH  - Databases, Genetic
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Ontology
MH  - *Gene Regulatory Networks
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics
MH  - ROC Curve
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics
PMC - PMC7780331
OTO - NOTNLM
OT  - competing endogenous RNA network
OT  - cytoplasm
OT  - laryngeal squamous cell carcinoma
OT  - long non-coding lncRNAs
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2020/12/30 06:00
MHDA- 2021/11/03 06:00
PMCR- 2020/12/28
CRDT- 2020/12/29 05:30
PHST- 2020/12/29 05:30 [entrez]
PHST- 2020/12/30 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2020/12/28 00:00 [pmc-release]
AID - 10.1177_1533033820985787 [pii]
AID - 10.1177/1533033820985787 [doi]
PST - ppublish
SO  - Technol Cancer Res Treat. 2020 Jan-Dec;19:1533033820985787. doi: 
      10.1177/1533033820985787.