PMID- 33372837
OWN - NLM
STAT- MEDLINE
DCOM- 20210924
LR  - 20210924
IS  - 2150-5608 (Electronic)
IS  - 2150-5594 (Print)
IS  - 2150-5594 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Dec
TI  - Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of 
      Streptococcus suis in a porcine precision-cut lung slice model.
PG  - 84-95
LID - 10.1080/21505594.2020.1858604 [doi]
AB  - Bordetella (B.) bronchiseptica and Streptococcus (S.) suis are major pathogens in 
      pigs, which are frequently isolated from co-infections in the respiratory tract 
      and contribute to the porcine respiratory disease complex (PRDC). Despite the 
      high impact of co-infections on respiratory diseases of swine (and other hosts), 
      very little is known about pathogen-pathogen-host interactions and the mechanisms 
      of pathogenesis. In the present study, we established a porcine precision-cut 
      lung slice (PCLS) model to analyze the effects of B. bronchiseptica infection on 
      adherence, colonization, and cytotoxic effects of S. suis. We hypothesized that 
      induction of ciliostasis by a clinical isolate of B. bronchiseptica may promote 
      subsequent infection with a virulent S. suis serotype 2 strain. To investigate 
      this theory, we monitored the ciliary activity by light microscopy, measured the 
      release of lactate dehydrogenase, and calculated the number of PCLS-associated 
      bacteria. To study the role of the pore-forming toxin suilysin (SLY) in S. 
      suis-induced cytotoxicity, we included a SLY-negative isogenic mutant and the 
      complemented mutant strain. Furthermore, we analyzed infected PCLS by 
      histopathology, immunofluorescence microscopy, and field emission scanning 
      electron microscopy. Our results showed that pre-infection with B. bronchiseptica 
      promoted adherence, colonization, and, as a consequence of the increased 
      colonization, the cytotoxic effects of S. suis, probably by reduction of the 
      ciliary activity. Moreover, cytotoxicity induced by S. suis is strictly dependent 
      on the presence of SLY. Though the underlying molecular mechanisms remain to be 
      fully clarified, our results clearly support the hypothesis that B. 
      bronchiseptica paves the way for S. suis infection.
FAU - Votsch, Desiree
AU  - Votsch D
AD  - Institute for Microbiology, University of Veterinary Medicine Hannover , 
      Hannover, Germany.
FAU - Willenborg, Maren
AU  - Willenborg M
AD  - Institute for Microbiology, University of Veterinary Medicine Hannover , 
      Hannover, Germany.
FAU - Baumgartner, Wolfgang
AU  - Baumgartner W
AD  - Institute for Pathology, University of Veterinary Medicine Hannover , Hannover, 
      Germany.
FAU - Rohde, Manfred
AU  - Rohde M
AD  - Central Facility for Microscopy, Helmholtz Center for Infection Research , 
      Braunschweig, Germany.
FAU - Valentin-Weigand, Peter
AU  - Valentin-Weigand P
AD  - Institute for Microbiology, University of Veterinary Medicine Hannover , 
      Hannover, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virulence
JT  - Virulence
JID - 101531386
RN  - 0 (Hemolysin Proteins)
RN  - 0 (suilysin)
SB  - IM
MH  - Animals
MH  - *Bacterial Adhesion
MH  - Bordetella bronchiseptica/genetics/*metabolism/*pathogenicity
MH  - Cilia/metabolism
MH  - Coinfection
MH  - Hemolysin Proteins/genetics
MH  - *Host-Pathogen Interactions
MH  - In Vitro Techniques
MH  - Lung/*microbiology
MH  - Streptococcus suis/metabolism/*pathogenicity
MH  - Swine
MH  - Swine Diseases/microbiology
PMC - PMC7781633
OTO - NOTNLM
OT  - Bordetella bronchiseptica
OT  - Streptococcus suis
OT  - PRDC
OT  - porcine precision-cut lung slices
OT  - porcine respiratory tract infection
OT  - respiratory co-infection
OT  - suilysin
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study, in the collection, analyses, or interpretation of the data, 
      in the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/12/30 06:00
MHDA- 2021/09/25 06:00
PMCR- 2020/12/29
CRDT- 2020/12/29 12:08
PHST- 2020/12/29 12:08 [entrez]
PHST- 2020/12/30 06:00 [pubmed]
PHST- 2021/09/25 06:00 [medline]
PHST- 2020/12/29 00:00 [pmc-release]
AID - 1858604 [pii]
AID - 10.1080/21505594.2020.1858604 [doi]
PST - ppublish
SO  - Virulence. 2021 Dec;12(1):84-95. doi: 10.1080/21505594.2020.1858604.