PMID- 33373507
OWN - NLM
STAT- MEDLINE
DCOM- 20201231
LR  - 20201231
IS  - 0869-2084 (Print)
IS  - 0869-2084 (Linking)
VI  - 65
IP  - 12
DP  - 2020 Dec 29
TI  - COVID-19 diagnostic laboratory strategies: modern technologies and development 
      trends (review of literature).
PG  - 757-766
LID - 10.18821/0869-2084-2020-65-12-757-766 [doi]
AB  - The COVID-19 pandemic, associated with the new coronavirus SARS-CoV-2, has caused 
      a surge in incidence worldwide, as well as a severe crisis in global health and 
      economy. Therefore, fast and accurate diagnosis of infection is key to timely 
      treatment and elimination of the spread of the virus. Currently, the standard 
      method for detecting coronavirus is reverse transcription polymerase chain 
      reaction (RT-PCR). However, this method requires expensive equipment and trained 
      personnel, which limits the conduct of mass testing and lengthens the time to 
      obtain a research result. Serological tests for antibodies against SARS-CoV-2 and 
      the determination of protective immunity in various populations are used to 
      retrospectively identify patients with asymptomatic and mild forms of infection, 
      monitor the course of infection in hospitalized patients, and also track contacts 
      and epidemiological surveillance. The use of standard methods for diagnosing 
      COVID-19 in conditions of mass morbidity, especially in conditions of 
      insufficient resources and lack of appropriate infrastructure, is associated with 
      a number of limitations. Therefore, the search and development of new, fast, 
      inexpensive, simple, device-free and no less sensitive and specific tests is an 
      urgent task. Therefore, the search and development of new, fast, inexpensive, 
      simple, device-free and no less sensitive and specific tests is an urgent task. 
      The review examines new laboratory technologies for diagnosing a new infection - 
      loop isothermal amplification (LAMP) and immunochromatographic analysis (ICA), 
      which can become a real alternative to the used molecular and enzyme immunoassay 
      methods. The dynamic development of these methods in recent years expands the 
      prospects for their use both for diagnosing COVID-19 and monitoring a pandemic.
FAU - Andryukov, Boris Georgievich
AU  - Andryukov BG
AUID- ORCID: 0000-0003-4456-808X
AD  - Somov Research Institute of Epidemiology and Microbiology, Russian Ministry of 
      Education and Science.
AD  - Far Eastern Federal University of the Ministry of Education and Science of 
      Russia.
FAU - Lyapun, I N
AU  - Lyapun IN
AUID- ORCID: 0000-0002-5290-3864
AD  - Somov Research Institute of Epidemiology and Microbiology, Russian Ministry of 
      Education and Science.
LA  - eng
PT  - Journal Article
PT  - Review
TT  - capital EL, Cyrillicsmall a, Cyrillicsmall be, Cyrillicsmall o, Cyrillicsmall er, Cyrillicsmall a, Cyrillicsmall te, Cyrillicsmall o, Cyrillicsmall er, Cyrillicsmall en, Cyrillicsmall yeru, Cyrillicsmall ie, Cyrillic small es, Cyrillicsmall te, Cyrillicsmall er, Cyrillicsmall a, Cyrillicsmall te, Cyrillicsmall ie, Cyrillicsmall ghe, Cyrillicsmall i, Cyrillicsmall i, Cyrillic small de, Cyrillicsmall i, Cyrillicsmall a, Cyrillicsmall ghe, Cyrillicsmall en, Cyrillicsmall o, Cyrillicsmall es, Cyrillicsmall te, Cyrillicsmall i, Cyrillicsmall ka, Cyrillicsmall i, Cyrillic COVID-19: small es, Cyrillicsmall o, Cyrillicsmall ve, Cyrillicsmall er, Cyrillicsmall ie, Cyrillicsmall em, Cyrillicsmall ie, Cyrillicsmall en, Cyrillicsmall en, Cyrillicsmall yeru, Cyrillicsmall ie, Cyrillic small te, Cyrillicsmall ie, Cyrillicsmall ha, Cyrillicsmall en, Cyrillicsmall o, Cyrillicsmall el, Cyrillicsmall o, Cyrillicsmall ghe, Cyrillicsmall i, Cyrillicsmall i, Cyrillic small i, Cyrillic small te, Cyrillicsmall ie, Cyrillicsmall en, Cyrillicsmall de, Cyrillicsmall ie, Cyrillicsmall en, Cyrillicsmall tse, Cyrillicsmall i, Cyrillicsmall i, Cyrillic 
      small er, Cyrillicsmall a, Cyrillicsmall ze, Cyrillicsmall ve, Cyrillicsmall i, Cyrillicsmall te, Cyrillicsmall i, Cyrillicsmall ya, Cyrillic (small o, Cyrillicsmall be, Cyrillicsmall ze, Cyrillicsmall o, Cyrillicsmall er, Cyrillic small el, Cyrillicsmall i, Cyrillicsmall te, Cyrillicsmall ie, Cyrillicsmall er, Cyrillicsmall a, Cyrillicsmall te, Cyrillicsmall u, Cyrillicsmall er, Cyrillicsmall yeru, Cyrillic).
PL  - Russia (Federation)
TA  - Klin Lab Diagn
JT  - Klinicheskaia laboratornaia diagnostika
JID - 9432021
RN  - LAMP assay
SB  - IM
MH  - COVID-19/*diagnosis
MH  - COVID-19 Testing/*methods
MH  - Chromatography, Affinity
MH  - Humans
MH  - Laboratories
MH  - Molecular Diagnostic Techniques
MH  - Nucleic Acid Amplification Techniques
MH  - *Pandemics
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - COVID-19
OT  - SARS-CoV-2
OT  - enzyme-linked immunosorbent assay
OT  - immunochromatographic analysis
OT  - loop isothermal amplification (LAMP)
OT  - reverse transcription polymerase chain reaction (RT-PCR)
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/30 06:00
MHDA- 2021/01/01 06:00
CRDT- 2020/12/29 17:10
PHST- 2020/12/29 17:10 [entrez]
PHST- 2020/12/30 06:00 [pubmed]
PHST- 2021/01/01 06:00 [medline]
AID - 10.18821/0869-2084-2020-65-12-757-766 [doi]
PST - ppublish
SO  - Klin Lab Diagn. 2020 Dec 29;65(12):757-766. doi: 
      10.18821/0869-2084-2020-65-12-757-766.