PMID- 33374317
OWN - NLM
STAT- MEDLINE
DCOM- 20210505
LR  - 20210505
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 11
IP  - 1
DP  - 2020 Dec 24
TI  - Development of Fluorescence In Situ Hybridization as a Rapid, Accurate Method for 
      Detecting Coliforms in Water Samples.
LID - 10.3390/bios11010008 [doi]
LID - 8
AB  - Coliform bacteria are indicators of water quality; however, most detection 
      methods for coliform bacteria are time-consuming and nonspecific. Here, we 
      developed a fluorescence in situ hybridization (FISH) approach to detect four 
      types of coliform bacteria, including Escherichia coli, Klebsiella pneumoniae, 
      Enterobacter aerogenes, and Citrobacter freundii, simultaneously in water samples 
      using specific probes for 16S rRNA. This FISH method was applied to detect 
      coliform bacteria in simulated water and domestic wastewater samples and compared 
      with traditional detection methods (e.g., plate counting, multiple-tube 
      fermentation (MTF) technique, and membrane filter (MF) technique). Optimal FISH 
      conditions for detecting the four types of coliforms were found to be fixation in 
      3% paraformaldehyde at 4  degrees C for 2 h and hybridization at 50  degrees C for 1.5 h. By 
      comparing FISH with plate counting, MTF, MF, and a commercial detection kit, we 
      found that FISH had the shortest detection time and highest accuracy for the 
      identification of coliform bacteria in simulated water and domestic wastewater 
      samples. Moreover, the developed method could simultaneously detect individual 
      species and concentrations of coliform bacteria. Overall, our findings indicated 
      that FISH could be used as a rapid, accurate biosensor system for simultaneously 
      detecting four types of coliform bacteria to ensure water safety.
FAU - Kuo, Jong-Tar
AU  - Kuo JT
AD  - Department of Biological Science and Technology, China University of Science and 
      Technology, Taipei 115, Taiwan.
FAU - Chang, Li-Li
AU  - Chang LL
AD  - Department of Horticulture and Landscape Architecture, National Taiwan 
      University, Taipei 106, Taiwan.
FAU - Yen, Chia-Yuan
AU  - Yen CY
AD  - Department of Biological Science and Technology, China University of Science and 
      Technology, Taipei 115, Taiwan.
FAU - Tsai, Teh-Hua
AU  - Tsai TH
AD  - Department of Chemical Engineering and Biotechnology, National Taipei University 
      of Technology, Taipei 10608, Taiwan.
FAU - Chang, Yu-Chi
AU  - Chang YC
AD  - Department of Biological Science and Technology, China University of Science and 
      Technology, Taipei 115, Taiwan.
FAU - Huang, Yu-Tang
AU  - Huang YT
AD  - Department of Biological Science and Technology, China University of Science and 
      Technology, Taipei 115, Taiwan.
FAU - Chung, Ying-Chien
AU  - Chung YC
AUID- ORCID: 0000-0002-0425-5444
AD  - Department of Biological Science and Technology, China University of Science and 
      Technology, Taipei 115, Taiwan.
LA  - eng
GR  - MOST 109-2313- B-157-001/Ministry of Science and Technology/
GR  - NSC 96-2815-C-157-003-E/National Science Council/
PT  - Journal Article
DEP - 20201224
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - *Bacteriological Techniques
MH  - Environmental Monitoring/*methods
MH  - Escherichia coli/isolation & purification
MH  - Fluorescence
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S
MH  - *Water Microbiology
PMC - PMC7824014
OTO - NOTNLM
OT  - biosensor
OT  - coliform bacteria
OT  - coliform detection method
OT  - fluorescence in situ hybridization
OT  - water safety
COIS- The authors declare no conflict of interest.
EDAT- 2020/12/31 06:00
MHDA- 2021/05/06 06:00
PMCR- 2020/12/24
CRDT- 2020/12/30 01:01
PHST- 2020/11/09 00:00 [received]
PHST- 2020/12/11 00:00 [revised]
PHST- 2020/12/24 00:00 [accepted]
PHST- 2020/12/30 01:01 [entrez]
PHST- 2020/12/31 06:00 [pubmed]
PHST- 2021/05/06 06:00 [medline]
PHST- 2020/12/24 00:00 [pmc-release]
AID - bios11010008 [pii]
AID - biosensors-11-00008 [pii]
AID - 10.3390/bios11010008 [doi]
PST - epublish
SO  - Biosensors (Basel). 2020 Dec 24;11(1):8. doi: 10.3390/bios11010008.