PMID- 33375137
OWN - NLM
STAT- MEDLINE
DCOM- 20210721
LR  - 20240330
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Dec 22
TI  - Cellular, Molecular and Biochemical Impacts of Silver Nanoparticles on Rat 
      Cerebellar Cortex.
LID - 10.3390/cells10010007 [doi]
LID - 7
AB  - BACKGROUND: The excessive exposure to silver nanoparticles (Ag-NPs) has raised 
      concerns about their possible risks to the human health. The brain is a highly 
      vulnerable organ to nano-silver harmfulness. The aim of this work was to evaluate 
      the impacts of Ag-NPs exposure on the cerebellar cortex of rats. METHODS: Rats 
      were assigned to: Control, vehicle control and Ag-NP-exposed groups (at doses of 
      10 mg and 30 mg/kg/day). Samples were processed for light and electron microscopy 
      examinations. Immunohistochemical localization of c-Jun N-terminal kinase (JNK), 
      nuclear factor kappa beta (NF-kappaB) and calbindin D28k (CB) proteins was performed. 
      Analyses of expression of DNA damage inducible transcript 4 (Ddit4), flavin 
      containing monooxygenase 2 (FMO2) and thioredoxin-interacting protein (Txnip) 
      genes were done. Serum levels of inflammatory cytokines were also measured. 
      RESULTS: Ag-NPs enhanced apoptosis as evident by upregulation of Ddit4 gene 
      expressions and JNK protein immune expressions. Alterations of redox homeostasis 
      were verified by enhancement of Txnip and FMO2 gene expressions, favoring the 
      activation of inflammatory responses by increasing NF-kappaB protein immune 
      expressions and serum inflammatory mediator levels. Another cytotoxic effect was 
      the reduction of immune expressions of the calcium regulator CB. CONCLUSION: 
      Ag-NPs exposure provoked biochemical, cellular and molecular changes of rat 
      cerebellar cortex in a dose-dependent manner.
FAU - Mohamed, Eman M
AU  - Mohamed EM
AUID- ORCID: 0000-0002-8047-9343
AD  - Department of Medical Histology and Cell Biology, Faculty of Human Medicine, 
      Zagazig University, Zagazig 44519, Egypt.
FAU - Kattaia, Asmaa A A
AU  - Kattaia AAA
AUID- ORCID: 0000-0002-7188-0100
AD  - Department of Medical Histology and Cell Biology, Faculty of Human Medicine, 
      Zagazig University, Zagazig 44519, Egypt.
FAU - Abdul-Maksoud, Rehab S
AU  - Abdul-Maksoud RS
AD  - Department of Medical Biochemistry, Faculty of Human Medicine, Zagazig 
      University, Zagazig 44519, Egypt.
FAU - Abd El-Baset, Samia A
AU  - Abd El-Baset SA
AD  - Department of Medical Histology and Cell Biology, Faculty of Human Medicine, 
      Zagazig University, Zagazig 44519, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20201222
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Biomarkers)
RN  - 0 (NF-kappa B)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Biomarkers/metabolism
MH  - Cerebellar Cortex/*drug effects
MH  - Male
MH  - Metal Nanoparticles/*toxicity
MH  - NF-kappa B/metabolism
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Silver/*toxicity
PMC - PMC7822184
OTO - NOTNLM
OT  - cerebellar cortex
OT  - impacts
OT  - rat
OT  - silver nanoparticles
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/12/31 06:00
MHDA- 2021/07/22 06:00
PMCR- 2020/12/22
CRDT- 2020/12/30 01:03
PHST- 2020/11/03 00:00 [received]
PHST- 2020/11/28 00:00 [revised]
PHST- 2020/12/17 00:00 [accepted]
PHST- 2020/12/30 01:03 [entrez]
PHST- 2020/12/31 06:00 [pubmed]
PHST- 2021/07/22 06:00 [medline]
PHST- 2020/12/22 00:00 [pmc-release]
AID - cells10010007 [pii]
AID - cells-10-00007 [pii]
AID - 10.3390/cells10010007 [doi]
PST - epublish
SO  - Cells. 2020 Dec 22;10(1):7. doi: 10.3390/cells10010007.