PMID- 33375878
OWN - NLM
STAT- MEDLINE
DCOM- 20210324
LR  - 20210324
IS  - 1607-8888 (Electronic)
IS  - 1025-3890 (Linking)
VI  - 23
IP  - 6
DP  - 2020 Nov
TI  - Low Fos expression in newly generated neurons of the main and accessory olfactory 
      bulb following single maternal separation.
PG  - 678-687
LID - 10.1080/10253890.2020.1828337 [doi]
AB  - The main and accessory olfactory bulbs (MOB and AOB) are unique in that they 
      produce new neurons throughout adulthood. Despite the recent knowledge about the 
      involvement of postnatally generated cells in several aspects of olfaction, the 
      functional role of these neurons is still not sufficiently understood. The 
      function of newly generated olfactory bulb neurons is primarily investigated in 
      relation to activities related to smell. Stress-induced activation of new 
      olfactory neurons has not yet been studied. Thus, our work was aimed to 
      investigate whether a stressful event, such as maternal separation (MS) can 
      induce Fos expression in postnatally-born neurons in the MOB and AOB. Rat pups 
      were exposed to single maternal separation (SMS) for 2 h at the postnatal days: 
      P7, P14, and P21. Quantification of immunohistochemically labeled Fos + cells 
      revealed that exposure to SMS in different age stages during the first postnatal 
      month stimulates activity in cells of individual MOB/AOB layers in an 
      age-dependent manner. In order to find out whether newly generated cells in the 
      MOB/AOB could express Fos protein as a response to SMS, newborn rats were 
      administrated with the marker of proliferation, bromodeoxyuridine (BrdU) at P0, 
      and three weeks later (at P21) colocalization of Fos and BrdU in the neurons of 
      the MOB and AOB was assessed. Quantitative analysis of BrdU/Fos double-labeled 
      cells showed that Fos is expressed only in a small number of postnatally 
      generated cells within the MOB/AOB. Our results indicate that postnatally 
      generated MOB/AOB neurons are less sensitive to stress caused by MS than 
      preexisting ones. LAY SUMMARY Our results showed that single maternal separation 
      (SMS) is a stressful event that in age-dependent manner stimulates cellular 
      activity in the main and accessory olfactory bulb (AOB) - the structures 
      dedicated to odor information processing. The low level of Fos expression in 
      newborn neurons of the main and accessory bulb indicates that postnatally 
      generated cells are less sensitive to neonatal stress than preexisting neurons.
FAU - Zavodska, Monika
AU  - Zavodska M
AUID- ORCID: 0000-0003-4615-100X
AD  - Department of Regenerative Medicine and Cell Therapy, Institute of Neurobiology, 
      Biomedical Research Center, Slovak Academy of Sciences, Kosice, Slovak Republic.
FAU - Fabianova, Kamila
AU  - Fabianova K
AUID- ORCID: 0000-0003-2965-4625
AD  - Department of Regenerative Medicine and Cell Therapy, Institute of Neurobiology, 
      Biomedical Research Center, Slovak Academy of Sciences, Kosice, Slovak Republic.
FAU - Martoncikova, Marcela
AU  - Martoncikova M
AUID- ORCID: 0000-0001-5418-1693
AD  - Department of Regenerative Medicine and Cell Therapy, Institute of Neurobiology, 
      Biomedical Research Center, Slovak Academy of Sciences, Kosice, Slovak Republic.
FAU - Racek, Adam
AU  - Racek A
AUID- ORCID: 0000-0001-9729-1921
AD  - Department of Anatomy, Histology and Physiology, University of Veterinary 
      Medicine and Pharmacy in Kosice, Kosice, Slovak Republic.
FAU - Racekova, Eniko
AU  - Racekova E
AUID- ORCID: 0000-0002-3650-2251
AD  - Department of Regenerative Medicine and Cell Therapy, Institute of Neurobiology, 
      Biomedical Research Center, Slovak Academy of Sciences, Kosice, Slovak Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201014
PL  - England
TA  - Stress
JT  - Stress (Amsterdam, Netherlands)
JID - 9617529
SB  - IM
MH  - Animals
MH  - *Maternal Deprivation
MH  - Neurons
MH  - *Olfactory Bulb
MH  - Rats
MH  - Smell
MH  - Stress, Psychological
OTO - NOTNLM
OT  - Adult neurogenesis
OT  - Fos protein
OT  - neonatal stress
OT  - olfactory system
OT  - postnatally generated neurons
OT  - single maternal separation
EDAT- 2020/12/31 06:00
MHDA- 2021/03/25 06:00
CRDT- 2020/12/30 05:15
PHST- 2020/12/30 05:15 [entrez]
PHST- 2020/12/31 06:00 [pubmed]
PHST- 2021/03/25 06:00 [medline]
AID - 10.1080/10253890.2020.1828337 [doi]
PST - ppublish
SO  - Stress. 2020 Nov;23(6):678-687. doi: 10.1080/10253890.2020.1828337. Epub 2020 Oct 
      14.