PMID- 33376492
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220419
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2020
DP  - 2020
TI  - Similar Repair Effects of Human Placenta, Bone Marrow Mesenchymal Stem Cells, and 
      Their Exosomes for Damaged SVOG Ovarian Granulosa Cells.
PG  - 8861557
LID - 10.1155/2020/8861557 [doi]
LID - 8861557
AB  - BACKGROUND: This study is aimed at investigating the repairing effect of 
      mesenchymal stem cells and their exosomes from different sources on ovarian 
      granulosa cells damaged by chemotherapy drugs-phosphoramide mustard (PM). 
      METHODS: In this study, we choose bone marrow mesenchymal stem cells (BMSCs) and 
      human placental mesenchymal stem cells (HPMSCs) for research. Then, they were 
      cocultured with human ovarian granulosa cells (SVOG) injured by phosphoramide 
      mustard (PM), respectively. beta-Galactosidase staining, flow cytometry, and Western 
      blot were used to detect the changes in the senescence and apoptosis of SVOG 
      cells before and after their coculture with the above two types of MSCs. 
      Subsequently, exosomes from these two types of MSCs were extracted and added to 
      the culture medium of SVOG cells after PM injury to test whether these two types 
      of exosomes played a role similar to that of MSCs in repairing damaged SVOG 
      cells. RESULTS: PM treatment-induced apoptotic SVOG cells were significantly 
      decreased after HPMSCs and BMSCs as compared with control group. After 
      coculturing with these two types of MSCs, PM-treated SVOG cells showed 
      significantly reduced senescence and apoptosis proportions as well as 
      cleaved-Caspase 3 expression, and HPMSCs played a slightly stronger role than 
      BMSCs in repairing SVOG cells in terms of the above three indicators. In 
      addition, the ratios of senescent and apoptotic SVOG cells were also 
      significantly reduced by the two types of exosomes, which played a role similar 
      to that of MSCs in repairing cell damages. CONCLUSIONS: The results indicated 
      that BMSCs, HPMSCs, and their exosomes all exerted a certain repair effect on 
      SVOG cells damaged by PM, and consistent repair effect was observed between 
      exosomes and MSCs. The repair effect of exosomes secreted from BMSCs and HPMSCs 
      on the SVOG cells was studied for the first time, and the results fully 
      demonstrated that exosomes are the key carriers for MSCs to play their role.
CI  - Copyright (c) 2020 Shuwen Chen et al.
FAU - Chen, Shuwen
AU  - Chen S
AUID- ORCID: 0000-0002-3846-9432
AD  - Reproductive Medical Center, Peking University People's Hospital, Peking 
      University, Beijing 100044, China.
FAU - Wang, Yanbin
AU  - Wang Y
AUID- ORCID: 0000-0001-7525-5057
AD  - Reproductive Medical Center, Peking University People's Hospital, Peking 
      University, Beijing 100044, China.
FAU - Liao, Liming
AU  - Liao L
AUID- ORCID: 0000-0002-9149-7432
AD  - School of Life Sciences, Peking University, Beijing 100191, China.
FAU - Meng, Li
AU  - Meng L
AUID- ORCID: 0000-0001-7233-6177
AD  - Incinta Fertility Center, Torrance, CA, USA.
FAU - Li, Juanjuan
AU  - Li J
AUID- ORCID: 0000-0003-3254-5008
AD  - Reproductive Medical Center, Peking University People's Hospital, Peking 
      University, Beijing 100044, China.
FAU - Shi, Cheng
AU  - Shi C
AUID- ORCID: 0000-0003-1412-9529
AD  - Reproductive Medical Center, Peking University People's Hospital, Peking 
      University, Beijing 100044, China.
FAU - Han, Hongjing
AU  - Han H
AUID- ORCID: 0000-0001-8883-8550
AD  - Reproductive Medical Center, Peking University People's Hospital, Peking 
      University, Beijing 100044, China.
FAU - Zheng, Xiaofeng
AU  - Zheng X
AUID- ORCID: 0000-0001-6790-0669
AD  - School of Life Sciences, Peking University, Beijing 100191, China.
FAU - Shen, Huan
AU  - Shen H
AUID- ORCID: 0000-0001-7242-7706
AD  - Reproductive Medical Center, Peking University People's Hospital, Peking 
      University, Beijing 100044, China.
LA  - eng
PT  - Journal Article
DEP - 20201203
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC7738794
COIS- The authors declare that they have no competing interests.
EDAT- 2020/12/31 06:00
MHDA- 2020/12/31 06:01
PMCR- 2020/12/03
CRDT- 2020/12/30 05:21
PHST- 2020/07/23 00:00 [received]
PHST- 2020/11/07 00:00 [revised]
PHST- 2020/11/18 00:00 [accepted]
PHST- 2020/12/30 05:21 [entrez]
PHST- 2020/12/31 06:00 [pubmed]
PHST- 2020/12/31 06:01 [medline]
PHST- 2020/12/03 00:00 [pmc-release]
AID - 10.1155/2020/8861557 [doi]
PST - epublish
SO  - Stem Cells Int. 2020 Dec 3;2020:8861557. doi: 10.1155/2020/8861557. eCollection 
      2020.