PMID- 33378036
OWN - NLM
STAT- MEDLINE
DCOM- 20210630
LR  - 20210630
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 24
DP  - 2020 Dec
TI  - LncRNA UCA1 stimulates the repair of hyperglycemic vascular smooth muscle cells 
      through targeting miR-582-5p.
PG  - 12859-12866
LID - 24188 [pii]
LID - 10.26355/eurrev_202012_24188 [doi]
AB  - OBJECTIVE: The purpose of this study was to elucidate the role of long non-coding 
      RNA (lncRNA) UCA1 in inducing the repair of hyperglycemic vascular smooth muscle 
      cells (VSMCs) by targeting microRNA-582-5p (miR-582-5p), thus alleviating 
      diabetic angiopathy. PATIENTS AND METHODS: Arterial vessels and serum exosomes 
      were collected from 40 type 2 diabetes mellitus (T2DM) patients and 40 non-T2DM 
      patients. Relative levels of UCA1 and miR-582-5p in collected samples were 
      detected. Then, the interaction between UCA1 and miR-582-5p was assessed by 
      Dual-Luciferase reporter assay. Moreover, the regulatory effects of UCA1 and 
      miR-582-5p on VSMCs phenotypes were determined. RESULTS: Results showed that 
      compared with non-T2DM patients, UCA1 was markedly downregulated, while 
      miR-582-5p was upregulated in VSMCs and serum exosomes of T2DM patients. They 
      exerted a negative expression correlation between each other. Besides, miR-582-5p 
      was the direct target of UCA1. Under the induction of increased doses of glucose, 
      UCA1 stimulated proliferative and invasive abilities in VSMCs. MiR-582-5p was 
      responsible for the repairability of UCA1 in VSMCs under the hyperglycemia state. 
      CONCLUSIONS: LncRNA UCA1 induces the repair of hyperglycemic VSMCs via negatively 
      regulating miR-582-5p. UCA1 may be a novel target for T2DM diagnosis and 
      treatment.
FAU - Yang, J-L
AU  - Yang JL
AD  - Department of Cardiovascular, Affiliated Hospital of Shandong University of 
      Traditional Chinese Medicine, Jinan, China. avie74r.mar@ksu.edu.bi.
FAU - Han, N-H
AU  - Han NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN582 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (UCA1 RNA, human)
SB  - IM
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Type 2/metabolism/pathology
MH  - Humans
MH  - Hyperglycemia/*metabolism/pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - Muscle, Smooth, Vascular/*metabolism/pathology
MH  - RNA, Long Noncoding/genetics/*metabolism
EDAT- 2020/12/31 06:00
MHDA- 2021/07/01 06:00
CRDT- 2020/12/30 12:42
PHST- 2020/12/30 12:42 [entrez]
PHST- 2020/12/31 06:00 [pubmed]
PHST- 2021/07/01 06:00 [medline]
AID - 24188 [pii]
AID - 10.26355/eurrev_202012_24188 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Dec;24(24):12859-12866. doi: 
      10.26355/eurrev_202012_24188.