PMID- 3338096
OWN - NLM
STAT- MEDLINE
DCOM- 19880316
LR  - 20190720
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 111
IP  - 2
DP  - 1988 Feb
TI  - Macrophage cell cycling: influence of proliferative state on the 
      antibody-mediated activities of rat resident peritoneal macrophages.
PG  - 492-500
AB  - Countercurrent centrifugal elutriation (CCE) was used to isolate fractions of rat 
      resident peritoneal macrophages that were enriched in different phases of the 
      cell cycle. The purpose was to assess the influence of the proliferative status 
      of these cells on their antibody-mediated phagocytic activity. Autoradiographic 
      analysis of the resident peritoneal cell population isolated 1 hr after an 
      intravenous injection of [3H] thymidine showed that about 3% of the macrophages 
      were in S-phase of the cell cycle. CCE yielded fractions of macrophages in which 
      the proportions of S-phase cells ranged from 0% to about 10%. Results of flow 
      cytometric analysis of propidium iodine-stained cells were consistent with the 
      autoradiographic findings. Essentially all of the macrophages in the CCE 
      fractions ingested antibody-coated particles, but there were marked differences 
      in phagocytic capacity and in expression of Fc-receptors among discrete groups of 
      cells. CCE fractions with the smallest cells and no S-phase macrophages ingested 
      approximately six- to eightfold fewer particles than did macrophages from CCE 
      fractions with the largest cells and enriched in S-phase macrophages. Similarly, 
      smaller macrophages bound fewer antibody-coated particles than did larger 
      macrophages. These results, which are identical to those previously reported for 
      murine macrophage cell lines, show that the number of Fc-receptors and the 
      phagocytic capacity of cycling resident peritoneal macrophages increase as the 
      cells progress from G1 to G2. Thus, the proliferative state of macrophages does 
      not determine whether they are phagocytic but rather their phagocytic capacity.
FAU - Walker, W S
AU  - Walker WS
AD  - Department of Immunology, St. Jude Children's Research Hospital, Memphis, 
      Tennessee 38101.
FAU - Beelen, R H
AU  - Beelen RH
LA  - eng
GR  - AI 17979/AI/NIAID NIH HHS/United States
GR  - CA 21765/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Receptors, Fc)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Cycle
MH  - Cell Fractionation
MH  - DNA/analysis
MH  - Macrophages/*cytology/immunology
MH  - Male
MH  - Peritoneal Cavity/cytology
MH  - Phagocytosis
MH  - Rats
MH  - Receptors, Fc/metabolism
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 0008-8749(88)90112-8 [pii]
AID - 10.1016/0008-8749(88)90112-8 [doi]
PST - ppublish
SO  - Cell Immunol. 1988 Feb;111(2):492-500. doi: 10.1016/0008-8749(88)90112-8.