PMID- 33383054
OWN - NLM
STAT- MEDLINE
DCOM- 20210302
LR  - 20210302
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 267
DP  - 2021 Feb 15
TI  - HUCMSCs transplantation combined with ultrashort wave therapy attenuates 
      neuroinflammation in spinal cord injury through NUR77/ NF-kappaB pathway.
PG  - 118958
LID - S0024-3205(20)31718-5 [pii]
LID - 10.1016/j.lfs.2020.118958 [doi]
AB  - AIMS: Spinal cord injury (SCI) is a major cause of long-term physical impairment. 
      Currently, treatment for SCI is limited to supportive measures, which can lead to 
      permanent disability, representing a serious social burden. The present study 
      aimed to evaluate the inflammatory microenvironment effects of human umbilical 
      cord mesenchymal stem cells (HUCMSCs)+ Ultrashort Wave (USW) therapy on SCI and 
      reveal possible mechanisms. MAIN METHODS: Low-dose USW was treated one day after 
      SCI, and HUCMSCs suspension was transferred to the lesion using a micro-syringe 
      7 days after SCI. The functional effects of HUCMSCs and USW, separately and 
      combinedly, were measured, together with the infiltration of CD3(+) cells, 
      formation of A1 astrocytes and activation of NUR77/ NF-kappaB pathway. KEY FINDINGS: 
      Our results showed that HUCMSCs+USW therapy improved motor function of SCI rat, 
      together with decreased infiltration of CD3(+) T cells, and decreased induction 
      of microglia and A1 astrocytes. And also USW treatment played a very important 
      role on decreasing the infiltration of CD3(+) T cells and IBA-1(+) cells. Reduced 
      production of pro-inflammatory cytokines IL-1beta and IL-6 was also observed in rats 
      receiving HUCMSCs+USW therapy, medicated by NUR77/NF-kappaB pathway. SIGNIFICANCE: 
      These findings indicated that HUCMSCs+USW therapy could attenuate inflammatory 
      microenvironment through NUR77/NF-kappaB signaling pathway, which might contribute to 
      its better outcome.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Wang, Shuai
AU  - Wang S
AD  - Department of Rehabilitation, Shengjing Hospital of China Medical University, 
      Shenyang 110004, People's Republic of China; Department of Rehabilitation, 
      Department of Rehabilitation Medicine, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou 510080, People's Republic of China.
FAU - Jia, Yiying
AU  - Jia Y
AD  - Department of Rehabilitation, Shengjing Hospital of China Medical University, 
      Shenyang 110004, People's Republic of China.
FAU - Cao, Xiangyan
AU  - Cao X
AD  - Kidney internal medicine, Tangdu Hospital of Air Force Military Medical 
      University, Xi'an, Shaanxi 710032, People's Republic of China.
FAU - Feng, Sining
AU  - Feng S
AD  - Department of Rehabilitation, Shengjing Hospital of China Medical University, 
      Shenyang 110004, People's Republic of China.
FAU - Na, Li
AU  - Na L
AD  - Department of Rehabilitation, Shengjing Hospital of China Medical University, 
      Shenyang 110004, People's Republic of China; Department of Neurology, People's 
      Hospital of Liaoning Province, Shenyang 116044, People's Republic of China.
FAU - Dong, Hongxuan
AU  - Dong H
AD  - Technology Department, Liaoning Qifu Stem Cell Biotechnology Co., Ltd., Shenyang 
      110136, People's Republic of China.
FAU - Gao, Jian
AU  - Gao J
AD  - Technology Department, Liaoning Qifu Stem Cell Biotechnology Co., Ltd., Shenyang 
      110136, People's Republic of China.
FAU - Zhang, Lixin
AU  - Zhang L
AD  - Department of Rehabilitation, Shengjing Hospital of China Medical University, 
      Shenyang 110004, People's Republic of China. Electronic address: 
      zhanglxs1974@163.com.
LA  - eng
PT  - Journal Article
DEP - 20201228
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cytokines)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Cord Blood Stem Cell Transplantation/*methods
MH  - Cytokines/metabolism
MH  - Female
MH  - Inflammation/pathology
MH  - Mesenchymal Stem Cells/metabolism/physiology/*radiation effects
MH  - Microglia/metabolism
MH  - NF-kappa B/metabolism
MH  - Neuroimmunomodulation/physiology
MH  - Oxidative Stress/drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Short-Wave Therapy/methods
MH  - Signal Transduction/drug effects
MH  - Spinal Cord/metabolism
MH  - Spinal Cord Injuries/metabolism/*therapy
MH  - Umbilical Cord
OTO - NOTNLM
OT  - HUCMSCs therapy
OT  - Inflammation
OT  - NUR77
OT  - Spinal cord injury
OT  - USW therapy
EDAT- 2021/01/01 06:00
MHDA- 2021/03/03 06:00
CRDT- 2020/12/31 20:09
PHST- 2020/06/22 00:00 [received]
PHST- 2020/12/06 00:00 [revised]
PHST- 2020/12/17 00:00 [accepted]
PHST- 2021/01/01 06:00 [pubmed]
PHST- 2021/03/03 06:00 [medline]
PHST- 2020/12/31 20:09 [entrez]
AID - S0024-3205(20)31718-5 [pii]
AID - 10.1016/j.lfs.2020.118958 [doi]
PST - ppublish
SO  - Life Sci. 2021 Feb 15;267:118958. doi: 10.1016/j.lfs.2020.118958. Epub 2020 Dec 
      28.