PMID- 33383564
OWN - NLM
STAT- MEDLINE
DCOM- 20210422
LR  - 20240226
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 537
DP  - 2021 Jan 22
TI  - Exogenous hydrogen sulfide inhibits neutrophils extracellular traps formation via 
      the HMGB1/TLR4/p-38 MAPK/ROS axis in hyperhomocysteinemia rats.
PG  - 7-14
LID - S0006-291X(20)32236-1 [pii]
LID - 10.1016/j.bbrc.2020.12.059 [doi]
AB  - Hydrogen sulfide (H(2)S) prevents platelet activation and neutrophils 
      extracellular traps (NETs) formation. However, the mechanism of sodium 
      hydrosulfide (NaHS, a donor that produces H(2)S) inhibits the formation of NETs 
      in hyperhomocysteinemia (HHcy) rats has not been previously investigated. In the 
      experiment, the expressions of HMGB1 of platelets, the expressions of TLR4, PAD4 
      and the phosphor-p38 of neutrophils were measured. The NETs formations, the 
      concentration of DNA in the serum and the culture solution of cultured 
      neutrophils which was stimulated by platelet-rich plasma (PRP) were tested. 
      Additionally, the cellular ROS level and SOD activity were detected. The 
      platelets were activated and the expression of HMGB1 of platelets and NETs 
      formation, the concentration of DNA, and the expressions of TLR4, phosphor-p38 
      and PAD4, the ROS level were all increased while the activity of SOD decreased in 
      the HHcy group compared to the control group. NaHS significantly inhibited the 
      activation of platelets, the production of ROS and the formation of NETs in 
      neutrophils, reversed the expressions of HMGB1, TLR4, phosphor-p38, PAD4 and 
      decreased concentration of DNA which was caused by high homocysteine. Our results 
      demonstrate that the donor of H(2)S inhibits NETs formation of neutrophils via 
      the HMGB1/TLR4/p38 MAPK/ROS pathway in hyperhomocysteinemia.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Zhao, Xueying
AU  - Zhao X
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital of Harbin 
      Medical University, Harbin, 150081, China.
FAU - Zhang, Liyuan
AU  - Zhang L
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital of Harbin 
      Medical University, Harbin, 150081, China.
FAU - Liu, Xin
AU  - Liu X
AD  - Department of Pathophysiology, Harbin Medical University, Harbin, 150080, China.
FAU - Zhao, Ziqing
AU  - Zhao Z
AD  - Department of Pathophysiology, Harbin Medical University, Harbin, 150080, China; 
      Department of Pathology, Zhongshan Hospital Affiliated to Xiamen University, 
      Xiamen, 361004, China.
FAU - Zhong, Xin
AU  - Zhong X
AD  - Department of Pathophysiology, Harbin Medical University, Harbin, 150080, China. 
      Electronic address: xzhong1111@hrbmu.edu.cn.
FAU - Wang, Yuwen
AU  - Wang Y
AD  - Department of Clinical Laboratory, The Second Affiliated Hospital of Harbin 
      Medical University, Harbin, 150081, China. Electronic address: yuww1111@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201228
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (HMGB1 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Toll-Like Receptor 4)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.5.3.15 (Protein-Arginine Deiminase Type 4)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Animals
MH  - Blood Platelets/drug effects/metabolism/ultrastructure
MH  - Disease Models, Animal
MH  - Extracellular Traps/drug effects/*metabolism
MH  - HMGB1 Protein/*metabolism
MH  - Hydrogen Sulfide/*pharmacology
MH  - Hyperhomocysteinemia/*metabolism
MH  - Male
MH  - Neutrophils/drug effects/metabolism
MH  - Phosphorylation/drug effects
MH  - Platelet-Rich Plasma/metabolism
MH  - Protein-Arginine Deiminase Type 4/metabolism
MH  - Rats, Wistar
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/drug effects
MH  - Toll-Like Receptor 4/*metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
MH  - Rats
OTO - NOTNLM
OT  - Exogenous hydrogen sulfide
OT  - High-mobility group box 1
OT  - Neutrophils extracellular traps
OT  - ROS
OT  - Toll-like receptor 4
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/01/01 06:00
MHDA- 2021/04/23 06:00
CRDT- 2020/12/31 20:18
PHST- 2020/12/16 00:00 [received]
PHST- 2020/12/17 00:00 [accepted]
PHST- 2021/01/01 06:00 [pubmed]
PHST- 2021/04/23 06:00 [medline]
PHST- 2020/12/31 20:18 [entrez]
AID - S0006-291X(20)32236-1 [pii]
AID - 10.1016/j.bbrc.2020.12.059 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2021 Jan 22;537:7-14. doi: 
      10.1016/j.bbrc.2020.12.059. Epub 2020 Dec 28.