PMID- 33384135
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1143
DP  - 2021 Jan 25
TI  - Paper-based upconversion fluorescence aptasensor for the quantitative detection 
      of immunoglobulin E in human serum.
PG  - 93-100
LID - S0003-2670(20)31166-1 [pii]
LID - 10.1016/j.aca.2020.11.036 [doi]
AB  - Immunoglobulin E (IgE), a biomarker of allergic diseases, plays a critical role 
      in allergic mechanism. Because of its low abundance in serum, the demand of 
      developing sensitive, selective and simple methods for IgE detection is still 
      very urgent. Paper-based analytical devices using upconversion nanoparticles 
      (UCNPs) as the label can be promising point-of-care test (POCT) methods in rapid 
      diagnosis, owing to their NIR-excitation and visible light emission nature, which 
      can avoid the interference of autofluorescence and scattering light from 
      biological samples and paper substrates. In this work, we proposed a paper-based 
      analytical device for the sensitive, selective and accurate detection of total 
      immunoglobulin E (IgE) in human serum. The assay was based on resonance energy 
      transfer between UCNPs and organic dye tetramethylrhodamine (TAMRA), and IgE 
      aptamer with stem-loop structure was used as the recognizing probe. The existence 
      of IgE change the conformation of IgE aptamer, enlarge the distance between donor 
      and acceptor, and block the energy transfer process. Thus, the luminescence of 
      UCNPs recovered with an IgE concentration independent manner. A linear 
      calibration was obtained in the range of 0.5-50 IU/mL, with a detection limit of 
      0.13 IU/mL. The results of our method were well correlated with that of 
      commercial ELISA kit (20 human serum samples). This work suggests promising 
      prospect of the paper-based UC-LRET analytical devices in real samples and may 
      promote the application of paper-based analytical devices in clinical diagnosis.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - He, Mengyuan
AU  - He M
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, 464000, China. Electronic address: myhe@xynu.edu.cn.
FAU - Shang, Ning
AU  - Shang N
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, 464000, China.
FAU - Zhu, Qianru
AU  - Zhu Q
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, 464000, China.
FAU - Xu, Jing
AU  - Xu J
AD  - College of Chemistry and Chemical Engineering, Xinyang Normal University, 
      Xinyang, 464000, China.
LA  - eng
PT  - Journal Article
DEP - 20201128
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Energy Transfer
MH  - Humans
MH  - *Immunoglobulin E
MH  - Limit of Detection
MH  - Luminescence
MH  - *Nanoparticles
OTO - NOTNLM
OT  - Aptasensor
OT  - Immunoglobulin E
OT  - Luminescence resonance energy transfer
OT  - Paper-based analytical devices
OT  - Upconversion luminescence
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/01/02 06:00
MHDA- 2021/05/15 06:00
CRDT- 2021/01/01 05:13
PHST- 2020/07/09 00:00 [received]
PHST- 2020/11/09 00:00 [revised]
PHST- 2020/11/23 00:00 [accepted]
PHST- 2021/01/01 05:13 [entrez]
PHST- 2021/01/02 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0003-2670(20)31166-1 [pii]
AID - 10.1016/j.aca.2020.11.036 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2021 Jan 25;1143:93-100. doi: 10.1016/j.aca.2020.11.036. Epub 
      2020 Nov 28.