PMID- 33384450
OWN - NLM
STAT- MEDLINE
DCOM- 20210510
LR  - 20220204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Dec 31
TI  - Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 
      for growth improvement in chemically defined and complex media.
PG  - 22448
LID - 10.1038/s41598-020-79904-9 [doi]
LID - 22448
AB  - Microbial induced calcite precipitation (MICP) based on ureolysis has a high 
      potential for many applications, e.g. restoration of construction materials. The 
      gram-positive bacterium Sporosarcina pasteurii is the most commonly used 
      microorganism for MICP due to its high ureolytic activity. However, Sporosarcina 
      pasteurii is so far cultivated almost exclusively in complex media, which only 
      results in moderate biomass concentrations at the best. Cultivation of 
      Sporosarcina pasteurii must be strongly improved in order to make technological 
      application of MICP economically feasible. The growth of Sporosarcina pasteurii 
      DSM 33 was boosted by detecting auxotrophic deficiencies (L-methionine, 
      L-cysteine, thiamine, nicotinic acid), nutritional requirements (phosphate, trace 
      elements) and useful carbon sources (glucose, maltose, lactose, fructose, 
      sucrose, acetate, L-proline, L-alanine). These were determined by microplate 
      cultivations with online monitoring of biomass in a chemically defined medium and 
      systematically omitting or substituting medium components. Persisting growth 
      limitations were also detected, allowing further improvement of the chemically 
      defined medium by the addition of glutamate group amino acids. Common complex 
      media based on peptone and yeast extract were supplemented based on these 
      findings. Optical density at the end of each cultivation of the improved peptone 
      and yeast extract media roughly increased fivefold respectively. A maximum OD600 
      of 26.6 +/- 0.7 (CDW: 17.1 +/- 0.5 g/L) was reached with the improved yeast extract 
      medium. Finally, culture performance and media improvement was analysed by 
      measuring the oxygen transfer rate as well as the backscatter during shake flask 
      cultivation.
FAU - Lapierre, Frederic M
AU  - Lapierre FM
AD  - Munich University of Applied Sciences, 80335, Munich, Germany. 
      frederic.lapierre@hm.edu.
FAU - Schmid, Jakob
AU  - Schmid J
AD  - Munich University of Applied Sciences, 80335, Munich, Germany.
FAU - Ederer, Benjamin
AU  - Ederer B
AD  - Munich University of Applied Sciences, 80335, Munich, Germany.
FAU - Ihling, Nina
AU  - Ihling N
AD  - Chair of Biochemical Engineering (AVT.BioVT), RWTH Aachen University, 52074, 
      Aachen, Germany.
FAU - Buchs, Jochen
AU  - Buchs J
AD  - Chair of Biochemical Engineering (AVT.BioVT), RWTH Aachen University, 52074, 
      Aachen, Germany.
FAU - Huber, Robert
AU  - Huber R
AD  - Munich University of Applied Sciences, 80335, Munich, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201231
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Culture Media)
RN  - 7440-44-0 (Carbon)
RN  - Sporosarcina pasteurii
SB  - IM
EIN - Sci Rep. 2022 Feb 1;12(1):2017. PMID: 35105924
MH  - *Bacterial Physiological Phenomena
MH  - Carbon/metabolism
MH  - *Culture Media/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Microbiological Techniques
MH  - *Nutritional Requirements
MH  - Sporosarcina/*physiology
PMC - PMC7775470
COIS- The authors declare no competing interests.
EDAT- 2021/01/02 06:00
MHDA- 2021/05/11 06:00
PMCR- 2020/12/31
CRDT- 2021/01/01 05:18
PHST- 2020/08/14 00:00 [received]
PHST- 2020/12/10 00:00 [accepted]
PHST- 2021/01/01 05:18 [entrez]
PHST- 2021/01/02 06:00 [pubmed]
PHST- 2021/05/11 06:00 [medline]
PHST- 2020/12/31 00:00 [pmc-release]
AID - 10.1038/s41598-020-79904-9 [pii]
AID - 79904 [pii]
AID - 10.1038/s41598-020-79904-9 [doi]
PST - epublish
SO  - Sci Rep. 2020 Dec 31;10(1):22448. doi: 10.1038/s41598-020-79904-9.