PMID- 33385245
OWN - NLM
STAT- MEDLINE
DCOM- 20210204
LR  - 20210204
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 166
IP  - 2
DP  - 2021 Feb
TI  - Genomic characteristics and pathogenicity of a new recombinant strain of porcine 
      reproductive and respiratory syndrome virus.
PG  - 389-402
LID - 10.1007/s00705-020-04917-8 [doi]
AB  - Recombination is an important phenomenon that accelerates evolution and enriches 
      the genetic diversity of porcine reproductive and respiratory syndrome virus 
      (PRRSV). Recombinant PRRSV isolates sometimes have different genetic backgrounds. 
      In this study, we report a recombinant PRRSV (SD-YL1712) isolated from a pig 
      farm. The genome of SD-YL1712 is 15,014 nucleotides in length, and its nucleotide 
      and amino acid sequence conservation is higher than that of PRRSV strain JXA1 
      except within the NSP2 region. The NSP2 region of SDYL1712 shares the highest 
      nucleotide (85.9%) and amino acid (84.1%) sequence identity with PRRSV strain 
      NADC30. SD-YL1712 was found to contain a characteristic 131-amino-acid deletion 
      in the NSP2 region. Two recombination breakpoints were detected at nt 2134 and nt 
      3958 within the NSP2 region, which revealed that SD-YL1712 originated from a 
      recombination event between NADC30-like and HP-PRRSV-derived MLV-like strains. 
      Interestingly, SD-YL1712 had an additional deletion at position 586, similar to 
      that found in strain TJnh1501. Moreover, the pathogenicity of strain SD-YL1712 
      was found to be similar to that of HP-PRRSV JXA1, which was higher than that of 
      the CH1a strain. Further analysis indicated that SD-YL1712 might be a 
      transitional intermediate in the evolution of TJbd1401 to TJnh1501.
FAU - Li, Yang
AU  - Li Y
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Xu, Gaoxiao
AU  - Xu G
AD  - Teaching and Research Section of Biotechnology, Nanning University, Nanning, 
      530200, Guangxi, China.
FAU - Du, Xingqian
AU  - Du X
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Xu, Lele
AU  - Xu L
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Ma, Zhiqian
AU  - Ma Z
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Li, Zhiwei
AU  - Li Z
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Feng, Yingtong
AU  - Feng Y
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Jiao, Dian
AU  - Jiao D
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Guo, Wenping
AU  - Guo W
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China.
FAU - Xiao, Shuqi
AU  - Xiao S
AUID- ORCID: 0000-0002-6468-2608
AD  - College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, 
      Yangling, 712100, Shaanxi, China. xiaoshuqi@nwsuaf.edu.cn.
LA  - eng
GR  - 2017YFD0500605/National Key Research and Development Program of China/
GR  - 31772764/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20210101
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China
MH  - Evolution, Molecular
MH  - Farms
MH  - Genetic Variation/genetics
MH  - Genome, Viral/*genetics
MH  - Genomics
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Recombination, Genetic/*genetics
MH  - Sequence Analysis, DNA/methods
MH  - Swine
MH  - Viral Nonstructural Proteins/genetics
MH  - Virulence/genetics
EDAT- 2021/01/02 06:00
MHDA- 2021/02/05 06:00
CRDT- 2021/01/01 12:02
PHST- 2020/07/07 00:00 [received]
PHST- 2020/10/30 00:00 [accepted]
PHST- 2021/01/02 06:00 [pubmed]
PHST- 2021/02/05 06:00 [medline]
PHST- 2021/01/01 12:02 [entrez]
AID - 10.1007/s00705-020-04917-8 [pii]
AID - 10.1007/s00705-020-04917-8 [doi]
PST - ppublish
SO  - Arch Virol. 2021 Feb;166(2):389-402. doi: 10.1007/s00705-020-04917-8. Epub 2021 
      Jan 1.