PMID- 33385611
OWN - NLM
STAT- MEDLINE
DCOM- 20211025
LR  - 20211025
IS  - 2210-3244 (Electronic)
IS  - 1672-0229 (Print)
IS  - 1672-0229 (Linking)
VI  - 18
IP  - 5
DP  - 2020 Oct
TI  - Landscape and Dynamics of the Transcriptional Regulatory Network During Natural 
      Killer Cell Differentiation.
PG  - 501-515
LID - S1672-0229(20)30166-2 [pii]
LID - 10.1016/j.gpb.2020.12.003 [doi]
AB  - Natural killer (NK) cells are essential in controlling cancer and infection. 
      However, little is known about the dynamics of the transcriptional regulatory 
      machinery during NK cell differentiation. In this study, we applied the assay of 
      transposase accessible chromatin with sequencing (ATAC-seq) technique in a 
      home-developed in vitro NK cell differentiation system. Analysis of ATAC-seq data 
      illustrated two distinct transcription factor (TF) clusters that dynamically 
      regulate NK cell differentiation. Moreover, two TFs from the second cluster, 
      FOS-like 2 (FOSL2) and early growth response 2 (EGR2), were identified as novel 
      essential TFs that control NK cell maturation and function. Knocking down either 
      of these two TFs significantly impacted NK cell differentiation. Finally, we 
      constructed a genome-wide transcriptional regulatory network that provides a 
      better understanding of the regulatory dynamics during NK cell differentiation.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Li, Kun
AU  - Li K
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China.
FAU - Wu, Yang
AU  - Wu Y
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China.
FAU - Li, Young
AU  - Li Y
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China.
FAU - Yu, Qiaoni
AU  - Yu Q
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China.
FAU - Tian, Zhigang
AU  - Tian Z
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China; CAS 
      Center for Excellence in Molecular Cell Sciences, The CAS Key Laboratory of 
      Innate Immunity and Chronic Disease, University of Science and Technology of 
      China, Hefei 230027, China.
FAU - Wei, Haiming
AU  - Wei H
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China; CAS 
      Center for Excellence in Molecular Cell Sciences, The CAS Key Laboratory of 
      Innate Immunity and Chronic Disease, University of Science and Technology of 
      China, Hefei 230027, China. Electronic address: ustcwhm@ustc.edu.cn.
FAU - Qu, Kun
AU  - Qu K
AD  - Department of Oncology, The First Affiliated Hospital of USTC, Division of 
      Molecular Medicine, Hefei National Laboratory for Physical Sciences at 
      Microscale, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230021, China; CAS 
      Center for Excellence in Molecular Cell Sciences, The CAS Key Laboratory of 
      Innate Immunity and Chronic Disease, University of Science and Technology of 
      China, Hefei 230027, China; School of Data Science, University of Science and 
      Technology of China, Hefei 230026, China. Electronic address: qukun@ustc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20201230
PL  - England
TA  - Genomics Proteomics Bioinformatics
JT  - Genomics, proteomics & bioinformatics
JID - 101197608
RN  - 0 (Chromatin)
SB  - IM
MH  - Cell Differentiation
MH  - *Chromatin
MH  - *High-Throughput Nucleotide Sequencing
MH  - Killer Cells, Natural
MH  - Sequence Analysis, DNA
PMC - PMC8377244
OTO - NOTNLM
OT  - ATAC-seq
OT  - Dynamic regulatory network
OT  - EGR2
OT  - FOSL2
OT  - NK cell
OT  - Programmed differentiation
EDAT- 2021/01/02 06:00
MHDA- 2021/10/26 06:00
PMCR- 2020/12/30
CRDT- 2021/01/01 20:08
PHST- 2018/08/01 00:00 [received]
PHST- 2018/12/10 00:00 [revised]
PHST- 2019/03/04 00:00 [accepted]
PHST- 2021/01/02 06:00 [pubmed]
PHST- 2021/10/26 06:00 [medline]
PHST- 2021/01/01 20:08 [entrez]
PHST- 2020/12/30 00:00 [pmc-release]
AID - S1672-0229(20)30166-2 [pii]
AID - 10.1016/j.gpb.2020.12.003 [doi]
PST - ppublish
SO  - Genomics Proteomics Bioinformatics. 2020 Oct;18(5):501-515. doi: 
      10.1016/j.gpb.2020.12.003. Epub 2020 Dec 30.