PMID- 33385954
OWN - NLM
STAT- MEDLINE
DCOM- 20210608
LR  - 20210608
IS  - 1872-6283 (Electronic)
IS  - 0379-0738 (Linking)
VI  - 319
DP  - 2021 Feb
TI  - Optimization of QuEChERS extraction for detection and quantification of 20 
      antidepressants in postmortem blood samples by LC-MS/MS.
PG  - 110660
LID - S0379-0738(20)30522-3 [pii]
LID - 10.1016/j.forsciint.2020.110660 [doi]
AB  - In this study, a comprehensively optimization of QuEChERS (quick, easy, cheap, 
      effective, rugged and safe) method using design of experiments (DOE) was 
      conducted to evaluate the best conditions to obtain the most effective 
      extraction. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) 
      analysis was performed to identify and quantify the antidepressants, with 
      electrospray ionization acquired in positive mode. The method was validated for 
      all analytes; the calibration curves were linear from 10-1000ng/mL, with 
      R(2)>0.98, and with LOD and LOQ defined as 10ng/mL. Method imprecision and bias 
      were less than 14.3% and 18.9%, respectively. Neither carryover nor interferences 
      were observed. Overall, the optimized method was applied in postmortem real 
      sample analysis to quantify the antidepressants. This study showed a viable 
      method that can be applied for routine forensic analysis, with a quick and easy 
      sample preparation and a rapid total run time of 8min for each analysis.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Campelo, Jacqueline de M
AU  - Campelo JM
AD  - Chemistry Department, Federal Rural University of Pernambuco, UFRPE, Recife, 
      Pernambuco, Brazil.
FAU - Rodrigues, Tais B
AU  - Rodrigues TB
AD  - Campinas Poison Control Center, Faculty of Medical Sciences, University of 
      Campinas, UNICAMP, Campinas, Sao Paulo, Brazil.
FAU - Costa, Jose L
AU  - Costa JL
AD  - Campinas Poison Control Center, Faculty of Medical Sciences, University of 
      Campinas, UNICAMP, Campinas, Sao Paulo, Brazil; Faculty of Pharmaceutical 
      Sciences, University of Campinas, UNICAMP, Campinas, Sao Paulo, Brazil.
FAU - Santos, Jandyson M
AU  - Santos JM
AD  - Chemistry Department, Federal Rural University of Pernambuco, UFRPE, Recife, 
      Pernambuco, Brazil. Electronic address: jandyson.machado@ufrpe.br.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20201229
PL  - Ireland
TA  - Forensic Sci Int
JT  - Forensic science international
JID - 7902034
RN  - 0 (Antidepressive Agents)
SB  - IM
MH  - Antidepressive Agents/*blood
MH  - Chromatography, Liquid
MH  - Forensic Toxicology/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Solid Phase Extraction
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Antidepressants
OT  - Design of experiments
OT  - Forensic toxicology
OT  - LC-MS/MS
OT  - Postmortem blood
OT  - QuEChERS
COIS- Declaration of Competing Interest The authors report no declarations of interest.
EDAT- 2021/01/02 06:00
MHDA- 2021/06/09 06:00
CRDT- 2021/01/01 20:16
PHST- 2020/07/22 00:00 [received]
PHST- 2020/12/09 00:00 [revised]
PHST- 2020/12/10 00:00 [accepted]
PHST- 2021/01/02 06:00 [pubmed]
PHST- 2021/06/09 06:00 [medline]
PHST- 2021/01/01 20:16 [entrez]
AID - S0379-0738(20)30522-3 [pii]
AID - 10.1016/j.forsciint.2020.110660 [doi]
PST - ppublish
SO  - Forensic Sci Int. 2021 Feb;319:110660. doi: 10.1016/j.forsciint.2020.110660. Epub 
      2020 Dec 29.