PMID- 3338994
OWN - NLM
STAT- MEDLINE
DCOM- 19880307
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 263
IP  - 4
DP  - 1988 Feb 5
TI  - Receptor-mediated activation of electropermeabilized neutrophils. Evidence for a 
      Ca2+- and protein kinase C-independent signaling pathway.
PG  - 1779-83
AB  - Electrically permeabilized neutrophils were used to study the mechanism of 
      activation of the respiratory burst by the chemotactic agent 
      formyl-methionyl-leucyl-phenylalanine (fMLP). Permeabilization was assessed by 
      flow cytometry, radioisotope trapping, and by the requirement for exogenous NADPH 
      for oxygen consumption. A respiratory burst could be elicited by fMLP, phorbol 
      ester, or diacylglycerol in permeabilized cells suspended in EGTA-buffered medium 
      with 100 nM free Ca2+. The fMLP response persisted even in cells depleted of 
      intracellular Ca2+ stores by pretreatment with ionomycin. Therefore, a change in 
      cytosolic free Ca2+ ([Ca2+]i) is not required for receptor-mediated stimulation 
      of the respiratory burst. The responses induced by phorbol ester and 
      diacylglycerol were largely inhibited by H7, a protein kinase C antagonist. In 
      contrast, the stimulation of oxygen consumption by fMLP was unaffected by H7. 
      These results suggest that a third signaling pathway, distinct from changes in 
      [Ca2+]i and activation of protein kinase C, is involved in the response of 
      neutrophils to chemoattractants.
FAU - Grinstein, S
AU  - Grinstein S
AD  - Division of Cell Biology, Hospital for Sick Children, Toronto, Canada.
FAU - Furuya, W
AU  - Furuya W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Diglycerides)
RN  - 0 (Ethers)
RN  - 0 (Isoquinolines)
RN  - 0 (Phorbol Esters)
RN  - 0 (Piperazines)
RN  - 53-59-8 (NADP)
RN  - 56092-81-0 (Ionomycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
MH  - Calcium/*metabolism
MH  - Cell Membrane Permeability
MH  - Diglycerides/pharmacology
MH  - Ethers/pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Ionomycin
MH  - Isoquinolines/pharmacology
MH  - Molecular Weight
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - NADP/metabolism
MH  - Neutrophils/*enzymology
MH  - Oxygen Consumption
MH  - Phorbol Esters/pharmacology
MH  - Piperazines/pharmacology
MH  - Protein Kinase C/*metabolism
EDAT- 1988/02/05 00:00
MHDA- 1988/02/05 00:01
CRDT- 1988/02/05 00:00
PHST- 1988/02/05 00:00 [pubmed]
PHST- 1988/02/05 00:01 [medline]
PHST- 1988/02/05 00:00 [entrez]
AID - S0021-9258(19)77944-7 [pii]
PST - ppublish
SO  - J Biol Chem. 1988 Feb 5;263(4):1779-83.